Corononostrongylus hasegawai, Purwaningsih & Dewi & Smales, 2019

Purwaningsih, Endang, Dewi, Kartika & Smales, Lesley R., 2019, Description of New Species of Coronostrongylus and Dorcopsistrongylus (Strongylida: Chabertiidae) from Dorcopsis muelleri (Macropodidae) from Kumawa Mountains, West Papua, Indonesia, Zootaxa 4565 (3), pp. 433-441 : 434-437

Corononostrongylus hasegawai n. sp.

( Figs 1–15 )

Description. General:Small nematodes, cuticle with fine transverse striations ( Fig. 14 ). Cephalic end with cephalic collar extended laterally, demarcated posteriorly by transverse annulations, width 86 (female, n=1). Collar pierced laterally by 2 large conspicuous amphids 41 apart (female, n=1) ( Figs. 3 , 11, 12 ), 4 smaller submedian papillae surrounding mouth opening knob-shaped, without seta ( Fig. 13 ). Internal aspect of cephalic collar continuous with lining of buccal cavity. Mouth opening circular, without lips or teeth, diameter 20 (female, n=1) ( Fig. 2 ). Buccal cavity length at least twice width; thin-walled, supported externally by heavily sclerotised annulus surrounding posterior third of cavity ( Fig. 2 ); lining of buccal cavity folded into 24 longitudinal pleats ( Fig. 4 ). Cervical alae absent. Oesophagus with cylindrical corpus, prominent isthmus and elongated bulb. Deirids tiny, setate, variable in position from level of posterior corpus to level of isthmus; nerve ring surrounding isthmus near excretory pore ( Fig. 1 ).

Male (n=10): Body length 6.98 (6.13–7.44) mm, maximum width 342 (300–390). Buccal capsule 42.1 (40–44) long, 20.8 (20–22) wide; annulus 14.3 (14–15) deep, 42.4 (40–45) internal diameter. Nerve ring, excretory pore, and deirid from cephalic end 515 (490–540), 540 (520–580), 464 (403–505), respectively. Oesophagus 755 (732– 778) long, 10.86 % (10.0–11.9) % body length; corpus 457 (430–470) long, 97 (92–107) wide; isthmus 70 (59–79) long, 57 (50–66) wide; bulb 185 (178–191) long, 154 (149–166) wide. Bursa short; internal surface covered with numerous refractile bosses, bosses lacking on posterior surface dorsal lobe. Ventro-ventral and latero-ventral rays apposed, long, slender, reach bursal margin; externo-lateral ray stout, divergent, shorter than other

lateral rays, not reaching bursal margin; medio-lateral and postero-lateral rays apposed, slender, reach bursal margin; externo-dorsal ray robust, arising adjacent to the lateral trunk, not reaching bursal margin; dorsal ray broad at origin, then dividing at mid-length,after principal bifurcation, internal branches slender, posteriorly directed, reaching bursal margin, external branches short, directed laterally, terminate in cuticular projections, not reaching bursal margin ( Fig. 5 ). Spicules slender, elongate, shaft tubular, 1061 (990–1160) long, 15.29% (13.3–18.6) % BL (Figs. 6,7), spicule ala begins from posterior 2/3 of spicule shaft, terminates anterior to spicule tip. Gubernaculum poorly sclerotized. Genital cone with small anterior conical lip; large posterior lip with a pair of simple conical appendages ( Fig. 8 ).

Female (n=10): Body length 8.21 (7.81–8.94) mm; maximum width 419 (390–420). Buccal capsule 43.3 (41– 46) long, 21.7 (20–24) wide; annulus 20.2 (19–22) deep, 55.3 (54–58) internal diameter. Nerve ring, excretory pore, and deirid from cephalic end 560 (530–570), 585 (560–620), 436 (330–528), respectively. Oesophagus 827 (810–855) long, 10.22 % (9.71–10.75) % BL; corpus 512 (490–550) long, 102 (99–106) wide; isthmus 75 (66–82) long, 67 (59–76) wide; bulb 197 (185–205) long, 175 (162–195) wide. Vulva close to anus, 836 (810–880) from tip of tail. Vagina 439 (400–498) long, vestibule 248 (227–274) long ( Fig. 10 ). Tail simple, conical, 526 (500–570) long (Figs.9,15). Eggs thin shelled, ellipsoidal, 85 (78.1–94.3) long, 47.0 (44.6–49.1) wide.

Type host: Dorcopsis muelleri (Lesson) (Mammalia: Macropodidae ).

Type locality: Kumawa Mountains , West Papua, Indonesia (1°6 S, 130°51 E).

Site of infection: Stomach.

Type specimens: Holotype male, allotype female ( MZB Na762); paratypes, 9 males and 9 females (MZBNa 763).

Date of collection: November 2014.

Other material examined: Voucher specimens 1 male, 1 female from D. muelleri from Salawati Island, Papua, 2009, (SAM AHC46516).

Etymology: The species epithet is dedicated to Dr. Hideo Hasegawa, an outstanding parasitologist who has contributed to the zoogeography of helminths in Indonesia.

Remarks: Following the keys of Beveridge (2002) Coronostrongylus hasegawai n.sp., with 24 longitudinal pleats in the buccal cavity, spicules less than 1,400 long and a poorly sclerotised gubernaculum, is most similar to C. spearei , the only other species described from New Guinea. Corononostrangylus hasegawai can be distinguished from C. spearei , however, as follows: the cephalic collar is extended laterally not being oval in shape; the buccal capsule is narrower, about half as wide as it is deep compared with about as wide as deep; the externodorsal ray of the bursa arises between the lateral and dorsal trunks, not from the lateral trunk; the external branches of the dorsal ray arise posteriorly to the principal bifurcation, not at about the same level; the females are larger (7.8–8.9 mm long compared with 5.55–7.92); the spicules are shorter (990–1,160 compared with 1,110– 1,230; 15.3% compared with 19.0% body length), females have a shorter vagina (400–498 compared with 510–660) a longer distance from vulva to tail tip (810–880 compared with 560– 800) and a longer tail (500– 570 compared with 350–490) ( Beveridge, 2002). Corononostrangylus hasegawai has been found only in D. muelleri from Papuan localities, while C. spearei has been found in Do. vanheurni , D. hageni , and D. luctuosa from Papua New Guinea ( Beveridge, 2002).

Figures

FIGURES 1–10. Coronostrongylus hasegawai n. sp. 1. Anterior end, lateral view. 2. Cephalic end, lateral view. 3. Cephalic end, apical view. 4. Optical transverse section through buccal capsule. 5. Bursa, dorsal view. 6. Proximal end of spicule. 7. Spicule tip, lateral view. 8. Genital cone. 9. Female tail, lateral view. 10. Vagina and ovejector, lateral view. Scale bars: 1, 100 µm; 2, 5, 6, 7, 50 µm; 3, 4, 8, 25 µm; 9, 10, 200 µm

FIGURES 11–15. Scanning electron micrographs of Coronostrongylus hasegawai n. sp. 11 & 12. Cephalic end, apical view. 13. Apical view showing longitudinal pleats, sub median papillae and amphid. 14. Fine transverse striations of cuticle on the mid region of the body. 15. Female tail, left lateral view. Scale bars: 11-14,10 µm; 15,150 µm