Meristotheca lysonensis X.-V. Nguyen, X.-T. Nguyen, Kittle & McDermid, 2022

Nguyen, Xuan-Vy, Nguyen, Xuan-Thuy & Kittle Iii, Ronald P., 2022, Meristotheca lysonensis sp. nov. (Solieriaceae, Rhodophyta), a new flattened species from Vietnamese waters, Phytotaxa 574 (2), pp. 137-148 : 141-144

Meristotheca lysonensis X.-V. Nguyen, X.-T. Nguyen, Kittle & McDermid sp. nov. ( Figs. 3–14 )

Holotype: VIETNAM. Quang Ngai: Ly Son Island ; 15.3743 ° N; 109.1329 ° E, 15 February 2021, coll. X.- V. Nguyen & X.- T. Nguyen (ION - CS 210215b, tetrasporophyte, Fig. 3 ). GenBank accession number for rbc L and COI-5 P: MZ 770759 and MZ 770756

Isotypes: VIETNAM. Ninh Thuan: Thai An ; 11.5648 ° N; 109.1269 ° E, 20 February 2021, coll. X.- V . Nguyen & X. - T . Nguyen (ION - CS 210301b, tetrasporophyte, Fig. 5 ). GenBank accession number for rbc L and COI-5 P: MZ770761 and MZ770758 . VIETNAM. Quang Ngai: Ly Son Island ; 15.3743 ° N; 109.1329 ° E, 02 April 2003, coll. H .- D. Nguyen & H.- T . Pham (ION -01011b, male plant, Fig. 6 ). GenBank accession number for rbc L and COI-5 P: MZ770760 and MZ770757

Habitat: Growing on dead corals in the mid-to lower intertidal zone where subjected to strong wave action.

Etymology: The specific epithet refers to Ly Son Island, the type locality of the new species in Viet Nam.

Description: Thalli prostrate or forming decumbent prostrate clumps; thallus attached to the substratum by many secondary holdfasts; thalli flattened, gelatinous in texture, deep rose-red to dark red, 10–20 cm tall with small papillae on the surface; divided into many broad segments in an irregularly dichotomous manner with rounded apices, becoming gradually narrower above and tapering below to a cuneate or sometimes cordate or heart-shaped base with a short slender stipe. Mature blades are 1–2 cm broad, margins entire at the beginning, later giving rise to irregularly branched obvious proliferations, broader or narrower intermixed; surface smooth, sometimes roughened by the presence of warty or short spinose processes ( Figs 3, 4 ), pinkish or yellowish when dried ( Figs 5, 6 ). Blades are 1.3–1.8 mm thick in the basal portions ( Fig. 7 ), becoming progressively thinner upward, 320–450 µm thick below the apices ( Fig. 8 ). Thalli are multiaxial and internally consist of a filamentous medulla. In traverse section, the outer cortex consists of 3– 4 layers of small, elongate cells, 12–20 µm in diameter, the inner cortex consists of 4–5 layers of bigger, ellipsoidal, or rounded cells, 40–140 µm in diameter. The medulla occupies about 20–30% of the blade thickness. Axial filaments run parallel to the longitudinal plane of the blade; adjacent axial filaments frequently linked by secondary pit-connections ( Fig. 9 ). Crosswise filaments originate from inner cortical cells and traverse the blade to connect with inner cortical cells of the opposite side ( Fig. 10 ). Tetrasporangia in scattered patches throughout the outer cortex on both dorsal and ventral sides of the entire blade except at tips and lowermost portions. Tetrasporangia are cut off from cortical cells located in the second layer of the outer cortex. In a few cases, tetrasporangial initials appear laterally, pit-connected to their parental cells ( Fig. 11 ). Tetrasporangial initials are basally attached to their supporting parental cortical cells ( Fig. 12 , solid triangle), and basal pit-connections remain in mature tetrasporangia. Tetrasporangia zonately divided, 10–15 µm diameter and 40–45 µm long ( Fig. 13 ). Spermatangia produced from the outermost cortical cells ( Fig. 14 ). Female gametophytes were not observed.

R emarks:A morphological comparison of M. lysonensis and other related species is given in Table 1 . Meristotheca lysonensis sp. nov. differs from M. coacta based on M. lysonensis’ larger size and greater blade thickness, blades that do not bear proliferations along the margins, and usually smooth blade margins. M. lysonensis clade habit is compressed to flattened. Pit-connections between tetrasporangial initials and the supporting parental cells change from lateral (young tetrasporangium) to basal (divided tetrasporangium). Meristotheca lysonensis also differs from M. procumbens in terms of thallus size and thickness, and the smaller medulla thickness in cross-sections. Position of pit-connections between tetrasporangial initials and the parental cells differ: basal position in M. lysonensis sp. nov., unlike lateral position in M. procumbens . This new species also differs from M. papulosa because M. lysonensis has many secondary holdfasts compared to single or a few erect blades arising from a discoid holdfast in M. papulosa . There are no branchlets in M. lysonensis whereas M. papulosa shows branchlets.

Figures

FIGURES 3–6. Meristotheca lysonensis X.-V. Nguyen, X.-T. Nguyen, Kittle et McDermid, sp. nov. 3: Wet habit of a vegetative thallus, holotype specimen (ION-CS210215b), tetrasporangial plant with secondary holdfasts (black arrows). 4. A mature segment of the blade, with the rough surface by the presence of warty or short spinose projections (ION-CS210215b). 5. Dried specimen, isotype, a male plant (ION-CS210301b). 6. Dried specimen, isotype (ION-01011b), tetrasporangial plant.

FIGURES 7–10. Vegetative structures of Meristotheca lysonensis X.-V. Nguyen, X.-T. Nguyen, Kittle et McDermid, sp. nov. (ION- CS210215b). 7. Cross section through the basal part showing the layers of outer layers (white arrow), inner layer cellular cortex (black arrow) and filamentous medulla (arrowhead). 8. Cross section near the apex of a blade. 9. Longitudinal section showing secondary pitconnections (arrowheads) between adjacent medullary cells. 10. Crosswise filaments (arrowhead) transversely arranged in the medullary region.

FIGURES 11–14. Reproductive structures of Meristotheca lysonensis X.-V. Nguyen, X.-T. Nguyen, Kittle et McDermid, sp. nov. (ION- CS210215b) 11. Tetrasporangial initial laterally pit-connected (arrowhead) to its parental cell. 12. Tetrasporangium basally pit-connected (arrowhead) to the parental cells. 13. Zonately divided tetrasporangium (arrowhead) with a basal pit-connection with the parental cell. 14. Spermatangia (arrowhead) produced from the outermost cortical cells (ION-CS210301b).