Paenibacillus shunpengii, Yang & Zhang & Chen & Cheng & Qiu & He & He, 2018

Yang, You-Jian, Zhang, Yan-Ting, Chen, Guo-Qiang, Cheng, Dan, Qiu, Ji-Guo, He, Qin & He, Jian, 2018, Paenibacillus shunpengii sp. nov., isolated from farmland soil, International Journal of Systematic and Evolutionary Microbiology 68 (1), pp. 211-216 : 215

DESCRIPTION OF PAENIBACILLUS SHUNPENGII SP. NOV.

Paenibacillus shunpengii (shun.peng′ i.i. N.L. gen. n. shunpengii of Shun-peng, to honour Shun-peng Li, a respected Chinese microbiologist, for his great contributions to China’ s environmental microbiology).

Cells are aerobic or facultatively anaerobic, Gram-stainpositive, motile, endospore-forming and rod-shaped (0.4– 0.6× 0.8–1.2 mm). Ellipsoidal spores are formed at terminal positions in swollen sporangia. After 24 h of growth on LB agar, colonies are circular, greyish, smooth, shiny and up to 5 mm in diameter. Grows at 18–42 Ǫ C (optimum 35 Ǫ C), pH 6.5–8.0 (optimum pH 7.5) and with 0.0–4.0 % (w/v) NaCl (optimum 0.5 %, w/v). In API 50 CH tests, acid is produced from L- arabinose, ribose, D- xylose, fructose, mannose, mannitol, N -acetylgulcosamine, amygdalin, arbutin, aesculin, salicin, celliobiose, maltose, lactose, melibiose, starch and potassium gluconate, but not from glycerol, erythritol, D-arabinose, L- xylose, adonite, xyloside, galactose, glucose, sorbose/sorbitose, rhamnose, dulcitol, inositol, D- sorbitol, methyl a- D- mannopyranoside, methyl a- D- glucopyranoside, sucrose, trehalose, inulin, melezitose, raffinose, glycogen, xylitol, gentiobiose, turanose, D- lyxose, D- tagatose, D-fucose, L- fucose, D- arabitol, L- arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. In API 20NE tests, utilizes aesculin, ferric citrate, gelatin, 4-nitrophenyl-b- D-galactopyranoside, L- arabinose, D- mannose, D- mannitol, N - acetylglucosamine, maltose and potassium gluconate, but not potassium nitrate, L- trypophan, D- glucose, L- arginine, urea, capric acid, adipic acid, malic acid, trisodium citrate or phenylacetic acid. In the API ZYM system, positive for cystine arylamidase and negative for esterase C4, esterase lipase C8, lipase C14, leucine arylamidase, valine arylamidase, trypsin, a- chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, a- galactosidase, b- galactosidase, b-glucuronidase, a- glucosidase, b- glucosidase, N -acetyl-b-glucosaminidase, a- mannosidase and a- fucosidase/fucosidase. Voges-Proskauer test is negative. The major cellular fatty acids are anteiso-C 15: 0, iso-C 16: 0 and anteiso-C 17: 0. The predominant respiratory quinone is MK-7. The polar lipid profile contains diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phospholipids. Cells are resistant to lincomycin, rifampin, novobiocin, nalidixic acid, bacitracin and polymyxin B.

The type strain, YYJ7-1 T (= ACCC 19965 T = KCTC 33849 T), was isolated from farmland soil in Wenzhou , Zhejiang Province, China (27 Ǫ 53′ N 120 Ǫ 36′ E). The DNA G+C content of the type strain is 39.4 mol% .