Neodolichodorus sinensis, Zhuo & Wang & Liao, 2010

Zhuo, Kan, Wang, Honghong & Liao, Jinling, 2010, Neodolichodorus sinensis sp. nov. (Nematoda: Dolichodoridae) from China, Zootaxa 2362 (1), pp. 63-68 : 63-67

Neodolichodorus sinensis sp. nov.

Figs. 1–31

Measurements. See Table 1.

Material examined. Type material: Holotype, adult female, slide NSF04 . Paratypes, adult females (slides NSF, NSF01 - NSF03 ); adult males ( NSM & NSM01 - NSM05 ); juveniles (slides NSJ13, NSJ15 & NSJ16, all putative stage 2 juveniles; NSJ01, NSJ08, NSJ14 & NSJ18-NSJ22, all putative stage 3 juveniles; NSJ03, NSJ04, NSJ06, NSJ07, NSJ09, NSJ10, NSJ23, all putative stage 4 juveniles) .

Type locality: Found in sandy soil samples collected from the rhizosphere of Kandelia candel in Futian Mangrove Reserve of Shenzhen, Guangdong Province, China (22º31’35.04’’ N, 113º59’53.77’’ E) . All specimens are deposited in Laboratory of Plant Nematology , South China Agricultural University, Guangzhou, China.

Description. Female. Body slightly ventrally curved to C-shaped when heat relaxed, tapering at both ends. Cuticle with striae about 1.6 µm apart at mid-body. Lateral fields with four incisures, originating behind lip region and ending on tail, not areolated at mid-body, irregularly areolated anteriorly and posteriorly, inner band obviously wider than outer bands posteriorly. Lip region continuous or slightly offset from body contour, bearing five to six transverse striae. Labial disc not prominent. Cephalic framework sclerotized. Stylet elongate, conus longer than shaft, basal knobs sloping posteriorly. Precorpus cylindrical, median bulb well developed with conspicuous valve, ovate in profile. Basal bulb elongate-pyriform. Excretory pore at level of median bulb, between base and valves. Hemizonid conspicuous, posterior to excretory pore, 192–235 µm from anterior end. Vulva a transverse slit, vagina almost one-half body width long, with asymmetrical vaginal sclerotization in lateral view. Two ovaries opposite and outstretched with oocytes in two or three rows. Tail elongate-conoid, tapering, slightly narrowing 13–15 annuli posterior to anus. Phasmids pore-like, level with or slightly anterior to anus, in middle of lateral field.

Male. Body similar to female in lip region, annulation, location of excretory pore, hemizonid, and lateral field, but slightly smaller. Testis outstretched, about 60% of body length. Bursa trilobed, lateral lobes rounded, crenate, prominently striated externally, extending from posterior to the head of the retracted spicules to just anterior to the tail tip and the maximum extent of the median lobe. Median lobe elongate-conoid, without spinules, terminus bifurcate or not. Spicules paired, slightly ventrally curved, bending at distal tip. Gubernaculum large, slender, straight, slightly protruding from cloaca. Gubernaculum accessory piece absent. Phasmids pore-like, located about half distance from cloacal aperture to tail tip. Tail short, pointed in lateral view.

Juvenile (all stages). Body similar to female except for reproductive system and location of excretory pore. Excretory pore about at level with anterior edge of basal bulb. Hemizonid invisible.

Diagnosis. Neodolichodorus sinensis sp. nov. is characterized by the continuous or slightly offset head with five to six transverse striae; female stylet 84–92 µm long (82.5–87.5 µm in male); female tail elongateconoid, slightly narrowing at 13–15 annuli posterior to anus; lateral fields with four lines; female phasmids pore-like at level with or slightly anterior to anus and relatively long spicules, between 60–70 µm; and a straight gubernaculum, between 32–33.8 µm.

Relationships. The new species differs from all other species of Neodolichodorus except N. leiocephalus Doucet, 1981 , N. paralongicaudatus, Rashid, 1990 and N. rageshi Siddiqi, 2000 in having a elongate-conoid female tail (all other species have a obtuse or mammillate tail). N. sinensis sp. nov. can be distinguished from the above three elongate-tailed species by the continuous or slightly offset head (vs. strongly set off from body).

In addition, the new species differs from N. leiocephalus in having five or six transverse striae on the head (vs. none), a more anterior vulva (v=49–50 vs. 53.3–57.7), longer female tail (c=25.6–30.9 vs. 39–52 and c’=3.3–3.6 vs. 1–2.1), and longer spicules (60–70 µm vs. 55–57 µm) and gubernaculum (31–33.8 µm vs. 21.5– 23.5 µm) in the male. It differs from N. paralongicaudatus in having the excretory pore located between the base and middle of median bulb in the female (vs. at basal part of isthmus), longer female stylet (84–92 µm vs. 73–75.5 µm), shorter female tail (c =25.6–30.9 vs. 21.2–21.5) and having phasmids level with or slightly anterior to anus in the female (vs. posterior to anus). From N. rageshi , the new species differs by the number of transverse striae on the head (five to six vs. none), longer female stylet (84–92 µm vs. 66 µm), anteriorly positioned vulva (V=49–50 vs. 56), and longer female tail (c=25.6–30.9 vs. 45.3 and c’=3.3–3.6 vs. 1.7).

Etymology. The specific name refers to the geographic location (adj. sinensis = Chinese, from China).

Figures

FIGURES 1–14. Neodolichodorus sinensis sp. nov. 1: Anterior part of female. 2: Anterior part of juvenile stage3. 3: Female head. 4: Lateral field in anterior body. 5: Lateral field in mid-body. 6: Lateral field in posterior body. 7: Vulval region and posterior gonad. 8: Female tail. 9: Juvenile stage2 tail. 10: Juvenile stage3 tail. 11. Juvenile stage4 tail. 12. Male tail. 13. Spicule and gubernaculum. 14. Male tail, ventral view.

FIGURES 15–31. Neodolichodorus sinensis sp. nov. 15: Female. 16: Male. 17–18: Anterior part of female.19: Male head. 20: Female head. 21: Excretory pore of juvenile stage3. 22: Excretory pore of female. 23: Vulval region. 24: Lateral field in anterior body. 25: Lateral field in mid-body. 26: Lateral field in posterior body. 27: Female tail. 28: Male tail, lateral view. 29: Spicules and gubernaculum. 30–31: Male tail, ventral view.