Phyllodistomum cribbi, León, Gerardo Pérez-Ponce De, Martínez-Aquino, Andrés & Mendoza-Garfias, Berenit, 2015

León, Gerardo Pérez-Ponce De, Martínez-Aquino, Andrés & Mendoza-Garfias, Berenit, 2015, Two new species of Phyllodistomum Braun, 1899 (Digenea: Gorgoderidae), from freshwater fishes (Cyprinodontiformes: Goodeidae: Goodeinae) in central Mexico: An integrative taxonomy approach using morphology, ultrastructure and molecular phylogenetics, Zootaxa 4013 (1), pp. 87-99 : 89-90

Phyllodistomum cribbi n. sp.

( Figs. 2–3 , 6–9 )

Synonym: Phyllodistomum sp. of Martínez-Aquino et al. (2012).

Type - Host. Zoogoneticus quitzeoensis (Bean) ; Goodeidae : Goodeinae ; picotee goodeid.

Type -Locality. Zacapu Lake, Michoacan, Mexico (19°49’35’’N, 101°47’10’’ W).

Other locality. La Luz Spring, Michoacan, Mexico (19º56’10.4”N, 102º17’57.84” W).

Other hosts. Goodeidae ; Goodeinae ; Allotoca zacapuensis Meyer, Radda & Domínguez-Domínguez ; and highland splitfin Hubbsina turneri de Buen (Zacapu); Goodeidae ; Goodeinae ; La Luz splitfin Zoogoneticus purhepechus Domínguez-Domínguez, Pérez-Rodríguez & Doadrio (La Luz Spring) .

Site of infection. Urinary bladder.

Specimens deposition. CNHE, Holotype 7815, paratypes: 9798, 7813, 7814, 7791.

Genbank accession numbers. COI ( KT376727 - KT376731 , ZACA Zqu1-5), 28S ( KT376718 - KT376722 , ZACA Zqu1-5

Etymology. The new species is named after Dr. Tom Cribb, University of

Queensland, Australia, for his great contribution to our understanding of fish trematode diversity, and particularly his contribution to the species descriptions of Phyllodistomum species.

Description. Measurements are of 10 gravid specimens. Body spatuled, with lateral margins smooth, 926– 1,841 (1,465) long, 477–1,224 (900) wide; maximum width at level of hindbody; body length/width 1.2–1.6 (1.5). Forebody relatively short, 423–665 (552) long, 323–590 (469) wide, representing 33–46% (39%) of total body length. Hindbody discoid, 237–1,201 (825) long, 477–1,224 (954) wide. Tegument with numerous tiny papillae. Oral sucker opening subterminally, rounded, 174–292 (239) long, 147–289 (239) wide, bearing eight welldeveloped papillae. Ventral sucker slightly wider than long, 145–252 (201) long, 149–277 (221) wide, bearing six well-developed papillae, one anterior, 4 lateral and 1 posterior. Oral sucker/ventral sucker length and width ratio 1:0.83–0.86 (1:0.84), 1:0.96–1.01 (1:0.92), respectively. Prepharynx and pharynx absent. Oesophagus very short (not visible in holotype), straight, 34–67 (55) long. Intestinal bifurcation 199–373 (296) from anterior end. Caeca wide, running laterally into the hindbody, ending blindly half the distance between testes and posterior end of body; right caecum 52–341 (220), left caecum 77–350 (221) from posterior end. Testes 2, entire to slightly lobed, oblique. Anterior testis 121–271(192) long, 96–267 (186) wide; posterior testis 80–300 (177) long, 104–283 (186) wide. Seminal vesicle saccular, relatively short. Pars prostatica not observed. Genital pore median, at level of caecal bifurcation, 262–439 (321) from anterior end. Ovary lobed (3–5 lobes), amphitypic, dextral in 6 specimens, sinistral in 2, lateral to anterior testis, 63–179 (110) long, 121–159 (139) wide. Two compact vitelline glands, ovoid to almost round, entire, immediately posterior to ventral sucker; right vitelline gland 48–93 (69) long, 62–146 (114) wide, left vitelline gland 54–100 (79) long, 60–143 (103) wide. Laurer´s canal not observed. Uterus extensively coiled, occupying all hindbody, inter- and extracaecal. Eggs oval to slightly rounded, 18–37 (28) long, 13–26 (20) wide. Excretory vesicle not observed. Excretory pore subterminal.

Figures

FIGURES 2 – 5. Line drawings of the holotype of P. cribbi n. sp. and P. wallacei n. sp., and detail of the male reproductive system of both species 2. Phyllodistomum cribbi n. sp. from Zoogoneticus quitzeoensis, ventral view. 3. Detail of the cirrus sac of P. cribbi n. sp. 4. Phyllodistomum wallacei n. sp. from Ilyodon furcidens, ventral view. 5. Detail of the cirrus sac of P. wallacei n. sp. Symbols: os = oral sucker, gp = genital pore, c = cecum, vs = ventral sucker, vg = vitelline gland, o = ovary, t = testis, e = eggs.

FIGURES 6 – 9. Scanning electron microscopy of a specimen of Phyllodistomum cribbi n. sp. 6. Adult, ventral view. 7. Oral sucker, showing 4 pairs of papillae. 8. Ventral sucker, showing 3 pairs of papillae. 9. Ventral surface of hindbody exhibiting papillae on the tegument.