Phyllodistomum wallacei, León, Gerardo Pérez-Ponce De, Martínez-Aquino, Andrés & Mendoza-Garfias, Berenit, 2015

León, Gerardo Pérez-Ponce De, Martínez-Aquino, Andrés & Mendoza-Garfias, Berenit, 2015, Two new species of Phyllodistomum Braun, 1899 (Digenea: Gorgoderidae), from freshwater fishes (Cyprinodontiformes: Goodeidae: Goodeinae) in central Mexico: An integrative taxonomy approach using morphology, ultrastructure and molecular phylogenetics, Zootaxa 4013 (1), pp. 87-99 : 91-92

Phyllodistomum wallacei n. sp.

( Figs. 4–5 , 10–13 )

Synonym: Dendrorchis sp. of Martínez-Aquino et al. (2009, 2014b).

Type-Host. Xenotaenia resolanae Turner ; Goodeidae : Goodeinae ; leopard splitfin. Type-Locality. Arroyo Durazno, Cuzalapa River, Jalisco, Mexico (19°30’32.1”N, 104°17’45.6”W). Other localities. Tamazula River, Jalisco, Mexico (19°43’22.7’’N,

103°12’08.5’’W), Ayuquila River, Jalisco, Mexico (19°37’15’’N, 104°12’10’’W)

Other hosts. Goodeidae : Goodeinae ; Tuxpan splitfin Allodontichthys tamazulae Turner (Tamazula) ;

Goodeidae : Goodeinae ; Goldbreast goodeid Ilyodon furcidens ( Jordan & Gilbert) (Ayuquila). Site of infection. Urinary bladder.

Specimens deposition. CNHE, Holotype 9799, paratypes 6881, 4786, 9800, 9801.

Genbank accession numbers. COI ( KT376723 - KT376726 , CUZA Xre1-2, TAMA Ata1-2), 28S ( KT376714 - KT376717 , CUZA Xre1-2, TAMA Ata1-2)

Etymology. The new species is named after Alfred Russell Wallace, co-discoverer of the Theory of Natural Selection and founding father of biogeography.

Description. Measurements are of 7 specimens. Body spatuled to elongate, with lateral margins smooth, 712– 1,619 (1,245) long, 445–1,222 (799) wide; maximum width at level of hindbody; body length/width 1.1–1.6 (1.5). Forebody relatively short, 355–650 (515) long, 187–506 (356) wide, representing 29–49% (39%) of total body length, bearing an irregular number of dome-like papillae on the ventral surface. Hindbody discoid to irregular, 554–1,223 (995) long, 488–1,045 (830) wide, bearing large number of dome-like papillae on the ventral surface. Oral sucker opening subterminally, rounded, 142–280 (194) long, 115–322 (198) wide, bearing 14 well-developed papillae, arranged in four pairs along the anterior edge, 1 pair in the middle, and 2 pairs along the posterior edge. Ventral sucker rounded, protuberant, 119–188 (160) long, 96–218 (165) wide, bearing six well-developed papillae, one anterior, 4 lateral and 1 posterior. Oral sucker/ventral sucker length and width ratio 1:0.67–0.84 (1:0.82), 1:0.67–0.84 (1:0.83), respectively. Prepharynx and pharynx absent. Oesophagus narrow, straight, 88–150 (119) long (not visible in the holotype). Intestinal bifurcation 271–349 (320) from anterior end. Caeca wide, running laterally into hindbody, ending blindly half distance between testes and posterior end of body; right caecum 131– 306 (232), left caecum 125–287 (218) from posterior end. Testes 2, lobed, oblique, smaller than ovary. Anterior testis 63–251 (124) long, 71–135 (105) wide; posterior testis 97–196 (134) long, 80–169 (112) wide. Seminal vesicle saccular, short. Pars prostatica not observed. Genital pore median, immediately post-bifurcal, 262–419 (334) from anterior end. Ovary deeply lobed (5 lobes), sinistral, lateral to anterior testis, usually larger than testes, 58–207 (135) long, 121–159 (139) wide. Two compact vitelline glands, bilobed, dorsal or immediately posterior to ventral sucker; right vitelline gland 36–115 (71) long, 38–133 (88) wide, left vitelline gland 35–116 (69) long, 39– 130 (88) wide. Laurer´s canal not observed. Uterus extensively coiled, occupying all hindbody, inter- and extracaecal. Eggs oval to slightly rounded, 19–32 (25) long, 13–23 (18) wide. Excretory vesicle not observed. Excretory pore subterminal.

Figures

FIGURES 2 – 5. Line drawings of the holotype of P. cribbi n. sp. and P. wallacei n. sp., and detail of the male reproductive system of both species 2. Phyllodistomum cribbi n. sp. from Zoogoneticus quitzeoensis, ventral view. 3. Detail of the cirrus sac of P. cribbi n. sp. 4. Phyllodistomum wallacei n. sp. from Ilyodon furcidens, ventral view. 5. Detail of the cirrus sac of P. wallacei n. sp. Symbols: os = oral sucker, gp = genital pore, c = cecum, vs = ventral sucker, vg = vitelline gland, o = ovary, t = testis, e = eggs.

FIGURES 10 – 13. Scanning electron microscopy of a specimen of Phyllodistomum wallacei n. sp. 10. Adult, ventral view, with scattered dome-like papillae on hind- and forebody. 11. Oral sucker, showing 7 pairs of papillae. 12. Ventral sucker, showing 3 pairs of papillae. 13. Dome-like papillae with small projections.