Bogga bogotensis ( Von Graff, 1899 ) Grau & Sluys & Froehlich & Carbayo, 2012

Grau, José Horacio, Sluys, Ronald, Froehlich, Eudóxia Maria & Carbayo, Fernando, 2012, Reflections on the genus Amaga Ogren and Kawakatsu 1990, and description of a new genus of land planarian (Platyhelminthes: Tricladida: Geoplanidae), Journal of Natural History 46 (25 - 26), pp. 1529-1546 : 1535-1542

publication ID	https://doi.org/ 10.1080/00222933.2012.691996
persistent identifier	https://treatment.plazi.org/id/03B787AA-FFAE-3A1E-FE2B-6212FE09C70D
treatment provided by	Felipe
scientific name	Bogga bogotensis ( Von Graff, 1899 )
status	comb. nov.

Treatment

Bogga bogotensis ( Von Graff, 1899) View in CoL , comb. nov.

Diagnosis

Broad leaf-like body; grey–black dorsal surface with a dark yellow marginal stripe that encircles the body and a dark yellow mid-dorsal stripe; both stripes about the same width; eyes located marginally; SMI: 9–10%; pharynx of the collar type; oesophagus small; globular prostatic vesicle located immediately posterior to pharyngeal pouch; small eversible penis; male atrium twice as long as female atrium; shell glands opening into the distal region of the oviducts. Oviducts ascending laterally to the gonopore. Vagina emerging from the posterodorsal wall of the genital atrium, with dorsal or anterodorsal orientation.

Material examined

Holotype: ZMB 743 View Materials . Preserved in 70% ethanol. Sections were prepared as follows: anterior region 1: transverse sections on seven slides; anterior region 2: sagittal sections on 11 slides; pre-pharyngeal region: transverse sections on four slides; pharynx: sagittal sections on 14 slides; copulatory apparatus: sagittal sections on 24 slides.

NHW 2741. Original material of G. bogotensis var. buergeri studied by Busson. Sagittal sections of the copulatory apparatus on 167 slides.

ZMA V.Pl. 6904.1. National Park , Bogotá, Colombia, 15 August 1975, coll. J. Tamsitt; front end: horizontal sections on 37 slides; pre-pharyngeal region: transverse sections on 13 slides; pharynx and copulatory apparatus: sagittal sections on 78 slides .

Description

Both ZMA V.Pl. 6904.1 and the holotype specimen have a broad, leaf-like body, with gradually tapering front end and the hind end rounded or obtuse. The holotype measured 33 mm in length, excluding the anterior body tip, which was absent ( Figure 3C View Figure 3 ). Judging from the shape of the body, the missing region may have measured 0.5–1 mm in length. The ZMA V.Pl. 6904.1 specimen measured 42 mm in length and 8 mm in width ( Figure 3B View Figure 3 ). The body reached its maximum width, 6–8 mm, at its median region. The mouth is at a distance from the anterior tip equivalent to 76% of body length, while the gonopore is located at 88% in the holotype ( Table 1). In both specimens the creeping sole comprises over 90% of the body width. The ground colour of the dorsal surface is grey–black in the ZMA V.Pl. 6904.1 animal; ventral surface whitish. A prominent dark yellow mid-dorsal stripe runs from the anterior tip to the posterior body margin. Dorsal surface also provided with a dark yellow marginal stripe that encircles the body ( Figure 3B View Figure 3 ) .

Eye cups multicellular, with the pigment cups measuring 38–40 µm in diameter. In the V.Pl. 6904.1 animal eyes are present in a single row at the anterior end ( Figure 3A View Figure 3 ), while from 1 or 2 mm behind the anterior margin the eye cups extend further backwards along the body margin in rows of three or four eyes on either side of the body. Sensory pits were only observed in the holotype, in which they are located ventrolaterally in a single row that encircles the anterior region of the body. The central nervous system consists of a ventral nerve plate ( Figure 4A View Figure 4 ) .

In both specimens the epidermis of the body margins is penetrated by granular xanthophil and fine erythrophil secretions. Rhabdite-forming cells are abundant in the dorsal parenchyma. Rhabditogen cells are also present ventrally, but only very scarcely. Cells with granular erythrophil and xanthophil secretions discharge their secretions through the ventral epidermis. Erythrophil cells discharge their granular secretion through the entire epidermis of the pre-pharyngeal region, i.e. through both ventral and dorsal regions. In the holotype, the creeping sole is clearly ciliated; this was not evident in the V.Pl. 6904.1 specimen, which may result from a fixation artefact .

Both V.Pl. 6904.1 and the holotype specimen show the three subepidermal muscle layers characteristic of the Geoplaninae ( Table 2): a subepidermal circular layer, followed by a double diagonal layer with decussate fibres, and then a longitudinal layer arranged in bundles. Thickness of subepidermal musculature relative to body height: SMI: 10.2% (holotype). In the anterior end of the body there are no glandular nor muscular specializations. In the pre-pharyngeal region of the holotype the ventral longitudinal muscle layer is thicker than the dorsal one (68 µm and 45 µm, respectively). In the V.Pl. 6904.1 specimen the dorsal subepidermal musculature (90 µm in thickness) is only slightly thicker than the ventral one (86 µm in thickness) .

In both specimens the parenchymal musculature consists of fibres arranged in various directions, but mainly orientated vertically (especially between the intestinal diverticles). Other parenchymal muscle fibres are arranged in three layers, namely a subintestinal transversal layer, a supra-intestinal transversal layer, and a dorsal double diagonal layer with decussate fibres ( Figure 4A,B View Figure 4 ). The parenchymal musculature does not form any specializations in the cephalic region.

The mouth is located in the middle of the pharyngeal pouch, the latter being surrounded by a single, subepithelial circular muscle layer. The collar-shaped pharynx ( Figure 4C View Figure 4 ) is highly folded on its free margin, filling the pouch almost completely. An oesophagus is present, but it was not completely observable in either of the specimens because it was not completely sectioned. The outer pharyngeal lining consists of a flat, cuboidal epithelium. The outer pharynx musculature is composed of a layer of intermingled longitudinal and circular fibres (about 5 µm in thickness). The outer epithelium of the distal region of the pharynx is penetrated by two types of secretory cells, producing erythrophil and xanthophil granules, respectively. The inner pharyngeal and oesophageal epithelia are underlain by a thin layer of longitudinal muscles (6 µm in thickness), followed by a much thicker layer of circular fibres (60 µm in thickness).

The globular testes measure about 200–250 µm in diameter. The follicles are located dorsally, arranged in five to seven rows on either side of the body. The testes are located between the intestinal branches, just beneath the supra-intestinal transversal muscle layer and extend from the level of the ovaries in the anterior region of the body to the root of the pharynx. In the holotype the most anterior and posterior testes are located, in relation to the anterior end, at 17% and 50% of body length, respectively.

The vasa deferentia run above the subintestinal transverse muscle layer and are located dorsally to the ovovitelline ducts ( Figure 4B View Figure 4 ). At the level of the prostatic vesicle the vasa deferentia bend dorsomedially and, subsequently, open separately into the anterolateral part of the prostatic vesicle. Posterior to the pharynx, the vasa deferentia are dilated to spermiducal vesicles that are full of spermatozoa. The vasa deferentia are lined with a squamous to cuboidal epithelium and are surrounded by a layer of circular muscle fibres (4 µm in diameter).

The ellipsoid prostatic vesicle is located directly behind the posterior wall of the pharyngeal pouch, at 0.2 mm and 0.6 mm in holotype and specimen V.Pl. 6904.1, respectively. The vesicle is lined with a flat, non-ciliated epithelium and is surrounded by a subepithelial layer of intermingled muscle fibres (45 µm in thickness). Numerous erythrophil finely granular secretions are discharged into the prostatic vesicle .

In the holotype, the prostatic vesicle does not have any open connection with the rest of the copulatory apparatus. In the region between the prostatic vesicle and the gonopore there are scattered fragments of muscle fibres roughly delimiting an elongated region that should be occupied by the penial bulb and the male atrium. This region contains tissue without cells or nuclei, without any signs of wall or cavity. Furthermore, there is no communication between this region and the female atrium, i.e. it is an isolated cavity that is continuous with the ample gonopore channel ( Figure 5D View Figure 5 ). Gonopore completely open.

In specimen V.Pl. 6904.1 the prostatic vesicle penetrates the common muscle coat of the copulatory apparatus and continues inside the penial bulb as a sinuous to slightly helicoidal ejaculatory duct. The latter opens into the male atrium through a small papilla. The ejaculatory duct is lined with tall, ciliated and nucleated cells, and is surrounded by a circular muscle layer; it does not receive any conspicuous secretions.

The male atrium of V.Pl. 6904.1 is a long and relatively narrow cavity, with its wall projecting into this cavity as numerous large, muscular and secretory papillae ( Figure 5B,C View Figure 5 ). These papillae are 300–400 µm tall and 150–250 µm wide at their base. A thick layer of circular muscle fibres, underlain by a longitudinal one, surrounds the male atrium in which the papillae are embedded. Each papilla also has its own circular musculature, as well as radial fibres running from one side of the papilla to the other. Two-thirds of each papilla is filled with a fine erythrophil secretion, originating from secretory cells located in the parenchyme anterior to the male atrium, albeit still located within the common muscular coat ( Figure 6A View Figure 6 ). The papillae have no lumen or cavity and the erythrophil secretion is discharged through the epithelium of each papillae ( Figure 6D View Figure 6 ).

The male atrium is almost completely filled with erythrophil secretions originating from these secretory papillae. It is lined with a low, ciliated epithelium, and is surrounded by intermingled longitudinal and circular muscle fibres. Male and female atria are clearly separated by a dorsal fold located on the male side, just above the gonopore ( Figure 5C View Figure 5 ). The opening of the male atrium is narrowed by a dorsal and ventral fold, slightly anterior to the gonopore. A muscular coat separately covers each atrium, as suggested by a clear muscular division at the level of the dorsal atrial fold that separates male and female atria.

In both animals the vitellarian follicles are relatively small, located dorsally and ventrally to the intestine in the parenchymal space delimited by the supra-intestinal and subintestinal parenchymal muscle layers, without invading the space between the intestinal branches.

The ovaries are large and elongate, up to 500 µm long by 200 µm wide. They are ventrally located above the subintestinal parenchymal muscle layer ( Figure 6C View Figure 6 ) at 5.6 mm from the anterior tip in the holotype. The ovovitelline ducts arise from the posterodorsal surface of the ovaries.

The oviducts run between the subintestinal muscle layer and the ventral nerve plate. At the level of the female atrium the ducts run dorsomedially and, subsequently, open into the vagina; common oviduct very small or absent. Ovovitelline ducts lined by a ciliated epithelium and surrounded by a layer of interwoven muscle fibres. Shell glands, producing a granular xanthophil secretion, open into the distal half of the ascending sections of the oviducts. After it has received the openings of the oviducts, the vagina dilates to form a cavity that is three to four times as wide as the oviducts. The female atrium is lined with a secretory epithelium. Granular xanthophil secretions fill the lumen of the female atrium, which is principally surrounded by longitudinal and circular muscle fibres.