Epeorus (Caucasiron) bicolliculatus Hrivniak, 2017

Hrivniak, Ľuboš, Sroka, Pavel, Godunko, Roman J. & Žurovcová, Martina, 2017, Mayflies of the genus Epeorus Eaton, 1881 s. l. (Ephemeroptera: Heptageniidae) from the Caucasus Mountains: a new species of Caucasiron Kluge, 1997 from Georgia and Turkey, Zootaxa 4341 (3), pp. 353-374 : 355-357

Epeorus (Caucasiron) bicolliculatus Hrivniak , sp. nov.

Figures 1–48

Epeorus alpicola ( Eaton, 1871) : Türkmen & Kazancı (2015: 44) [partim] Epeorus sylvicola ( Pictet, 1865) : Türkmen & Kazancı (2015: 44, 45) [partim] Epeorus (Caucasiron) sp.: Martynov et al. (2016: 170)

Type material. Locality numbers (in square brackets) correspond to numbers on Fig. 51 . Collectors of type material are listed in section Material and methods.

Holotype: female mature larva, [1.] GEORGIA: Autonomous Republic of Adjara (hereafter Adjara), vicinity of Chakhati village, Kintrishi Riv. (41°45'43"N 41°58'34"E), 325 m a.s.l., Martynov A.V. leg. 18– 20.4.2016 (preserved in 96% EtOH).

Paratypes: 1 female, 1 male imago, 1 male subimago (all reared from larvae and mounted on slides), 1 male, 6 female larvae (2 mounted on slide), same place and date as holotype [1.]; same place and date as holotype [1.]; 2 female larvae, GEORGIA, Adjara, vicinity of Chakhati village, Kintrishi Riv. (41°45'42"N 41°58'42"E; close to holotype locality), 325 m a.s.l., 6.6.2016 ; 12 female larvae (1 mounted on slide, 3 with only mouthparts mounted on slide), 1 male larva (mounted on slide), [2.] GEORGIA : Adjara, vicinity of Chakhati village , right tributary of Kintrishi Riv. (41°44'14"N 41°59'00"E), 380 m a.s.l., 19.4.2016 ; 1 male larva, [4.] GEORGIA : Imereti Region (hereafter Imereti), vicinity of Tskhomareti village , Jruchula Riv. (42°23'12.8"N 43°21'33.3"E), 505 m a.s.l., 23.6.2013; 4 male , 5 female larvae (1 mounted on slide), [5.] GEORGIA : Imereti, right tributary of Sairmegele Riv. (41°52'57.7"N 42°45'22.6"E), 1276 m a.s.l., 25.6.2013 ; 2 female larvae (1 mounted on slide), [8.] GEORGIA : Imereti, Sairmegele Riv. and its right tributary (41°52'45.2"N 42°45'32.2"E), 1285 m a.s.l., 25.6.2013 ; 1 female larva, [9.] GEORGIA : Imereti, app. 2 km S. Health Resort Sairme, Sairmegele Riv. (41°52'56"N 42°45'09"E), 1262 m a.s.l., 25.6.2013; 1 male, 2 female larvae (1 mounted on slide), GEORGIA : Adjara, vicinity of Chakhati village , right tributary of Kintrishi Riv. (41°45'23"N 41°58'41"E; close to holotype locality), 385 m a.s.l., 7.6.2013 ; 1 female larva, [12.] GEORGIA : Guria Region, Ozurgeti (41°54'29.64"N 42°03'52.44"E), 169 m a.s.l., 22.8.2009 ; 1 female larva, [13.] GEORGIA : Adjara, Zeda Erge village, near bridge on road Batumi –Akhaltsikhe, Jochostskali Riv. (right tributary of Tchorokhi Riv. ) (41°34'02.04"N 41°41'16.20"E), 40 m a.s.l., 21.8.2009 ; 2 female larvae, [14.] GEORGIA : Adjara, Adzarisagmarti village , left tributary of Machakhlistskali Riv. (41°30'37.20"N 41°49'18.06"E), 207 m a.s.l., 20.8.2009 ; 1 female larva, [15.] TURKEY: Trabzon Province, vicinity of Hamşiköy village, right tributary of Maçka Riv. (40°40'45.36"N 39°26'55.74"E), 1460 m a.s.l., 29.6.2011 ; 1 female larva, [16.] GEORGIA : Adjara, Khala village , Kemerkobis Riv. (right tributary of Chekvistskali Riv.) (41°42'08.70"N 41°48'51.30"E), 100 m a.s.l., 18.8.2009; 5 female larvae (2 mounted on slides) , 1 male larva (mounted on slide) [19.] TURKEY: Giresun Province, Kümbet Plateau, small tributary of Aksu Stream (40°32'19''N 38°21'35'' E), 1570 m a.s.l., 02.6.2009 ; 2 female, 2 male larvae, [20.] TURKEY: Artvin Province, Artvin town, Gorgit Plateau Road, Didrelle Creek (41°25'13.65"N 41°55'29.57"E), 922 m a.s.l., 29.7.2016 .

Other material examined (not type): 1 male larva, [3.] GEORGIA : Adjara, vicinity of Khino village , Kintrishi Riv. (41°43'01"N 42°02'41"E), 794 m a.s.l., 19.4.2016; 4 female, 3 male larvae, [5.] ; 1 juv. larva, [6.] GEORGIA : Adjara, Kokoleti village , Machakhlistskali Riv. (41°29'54.7"N 41°51'26.1"E), 273 m a.s.l., 17.6.2013 ; 1 female larva, [7.] GEORGIA : Imereti, small stream, road Sairme-Abastumani (41°51'49.0"N 42°47'21.1"E), 1804 m a.s.l., 30.6.2013 ; 1 male larva, [10.] GEORGIA : Racha-Lechkhumi and Kvemo Svaneti Region, Legvanta village , Sakora Riv. (42°36'32.28"N 43°27'45.18"E), 837 m a.s.l., 25.8.2009 ; 4 juv. larvae, [11.] GEORGIA : Adjara, app. 5 km E. Goderdzi Pass, left tributary of Dzindzinstskali Riv. (41°38'00.18"N 42°33'24.6"E), 1801 m a.s.l., 28.8.2009; 1 female larva, [14.]; 1 female larva, [15.]; 1 female larva, ARMENIA: Kotayk Province, Geghard, Azat Riv. , July 1975 ; 4 female larvae, [17.] TURKEY: Giresun Province, Dereli-Kümbet Road, Aksu Stream (40°41'01.08"N 38°26'29.71"E), 557 m a.s.l., 1.6.2009 ; 1 female larva [18.] TURKEY: Giresun Province, Kümbet Plateau, Aksu Stream , (40°32'22''N 38°24'06''E), 1542 m a.s.l., 2.6.2009 .

Figures

FIGURE 51. Map showing sampling sites with records of Epeorus (Caucasiron) bicolliculatus sp. nov. (locality numbers correspond to numbers given in square brackets in the list of material studied).

FIGURES 1 – 12. Epeorus (Caucasiron) bicolliculatus sp. nov., larva: 1, female (dorsal); 2, female (ventral); 3, female (lateral); 4, male head (dorsal); 5, female head (dorsal); 6, male head (frontal view; labrum in natural position); 7 – 9, colouration of sterna II – V; 10, colouration of terga I – X; 11, tergal protuberances on terga VII – IX; 12, gill plates VII.

FIGURES 13 – 15. Epeorus (Caucasiron) bicolliculatus sp. nov., mouthparts (flattened on slide): 13, right mandible incisors (ventral); 14, left mandible incisors (ventral); 15, labrum (left part ventral; right part dorsal).

FIGURES 16 – 28. Epeorus (Caucasiron) spp.: 16 – 22 a, E. (C.) bicolliculatus sp. nov., gills I – VII (flattened on slide); 22 b gill plate VII in natural position from ventral view; 23 – 25, tergum VII (flattened on slide; vertical line represents midline of tergum): 23, E. (C.) bicolliculatus sp. nov.; 24; E. (C.) longimaculatus (paratype); 25, E. (C.) soldani; 26, E. (C.) bicolliculatus sp. nov., femoral spatulate seta (medial area of middle femur) (flattened on slide); 27, E. (C.) bicolliculatus sp. nov., IX sternum of female; 28, E. (C.) bicolliculatus sp. nov., IX sternum of male.

FIGURES 29 – 34. Epeorus (Caucasiron) bicolliculatus sp. nov., larva, egg, subimago: 29, blade-like setae and bluntly pointed spine-like setae along posterior margin of femora (larva); 30, basally widened setae on tergum VII (larva); 31, tergal protuberances on terga VI – VIII (larva); 32, tergal protuberances in detail (larva); 33 a, egg; 33 b, detail of chorionic surface and micropyle; 34, detail of microlepides and microtrichia of 4 th segment of foretarsus (male subimago).

FIGURES 35 – 46. Epeorus (Caucasiron) bicolliculatus sp. nov., imago: 35, male head; 36, female head; 37, middle leg; 38, male abdomen (lateral); 39; male abdomen (dorsal); 40, male abdomen (ventral); 41, female abdomen (lateral); 42, female abdomen (dorsal); 43, female abdomen (ventral); 44 – 45, detail of colouration pattern (sterna V – VI); 46 a, b, subgenital plate and sternum IX (female).

FIGURES 47 – 48. Epeorus (Caucasiron) bicolliculatus sp. nov., male genitalia: 47, imago; 48 a, subimago; 48 b, subimaginal titillator in detail (apex).