Amanita cingulata J. W. Liu & Zhu L. Yang, 2017

Liu, Jian-Wei, Cai, Qing, Cui, Yang-Yang & Yang, Zhu L., 2017, Amanita cingulata, a new annulate species of Amanita sect. Vaginatae from subtropical China, Phytotaxa 326 (1), pp. 41-53 : 47-49

Amanita cingulata J. W. Liu & Zhu L. Yang , sp. nov. ( Figs. 3–4 )

MycoBank MB 820987 Etymology:— cingulatus, cingulate, referring to the annulus of the species. Holoype:— CHINA. Hunan Province: Zhuzhou County, Yanling Scenic Spot, on the ground in a mixed forest dominated by members of

Fagaceae and Theaceae , 26°19′12″N 114°00′28″E, elev. 1516 m, 22 July 2012, Q. Cai 866 (HKAS75600, GenBank Acc. No.: nrLSU

= KY949583, ITS = KY949582).

Description:— Pileus 4–5.5 cm in diam, plano-convex to applanate, white to cream-colored (3A1–2); pileal margin shortly striate (ca. 0.2–0.3R); volval remnants on the pileus cracking into irregular, cushion-like or nubbly patches with maturity, white to snow white (1A1). Lamellae free, crowded, white to cream (1A1); lamellulae truncate, in 2–3 tiers; lamellar edges white. Stipe 6–8 × 0.9–1.7 cm, nearly cylindric, usually taping upwards, with apex slightly expanded, white (5A1) to cream (5A2), covered with snake-skin shaped, white squamules above annulus, and decorated with minute, sometimes recurved floccules to fibrils under annulus; context white, hollow in center; basal bulb absent; annulus present, thin, membranous, white (1A1), finely and radially radiate above, nearly smooth below, superior, easily detached from stipe; volva on the stipe base saccate, but breaking into lumpy volval remnants as on the pileus, white to snow white (1A1). Odor indistinct.

Lamellar trama bilateral.Mediostratum 30–70 μm wide, composed of fairly abundant, long ellipsoid to subfusiform inflated cells (20–40 × 11–30 μm) and abundant interwoven, often anastomosing, filamentous hyphae 3–8 μm wide; vascular hyphae rare, 3–7 μm wide. Lateral stratum composed of fairly abundant, long ellipsoid to subfusiform inflated cells (20–50 × 10–20 μm), diverging at an angle of ca. 30–50° to mediostratum; filamentous hyphae abundant, 2–7 μm wide, frequently branching, interwoven, sometimes anastomosing; vascular hyphae rare, 2–7 μm wide. Subhymenium 20–40 (–50) μm thick, with 2–3 layers of subglobose to ovoid or irregularly shaped cells 3–12 × 8–16 μm, sometimes mixed with a few slightly inflated cells 3–7 μm wide. Basidia 43–50 × 7–11 μm, clavate, 4–spored; sterigmata 4–5 μm long; basal septa without clamp connections. Basidiospores [200/2/2] (7.5–) 9–11.5 (–12) × (5–) 5.5–7 (8.5) μm (Q = (1.25) 1.4–1.83 (–2.0), Qm = 1.58 ± 0.13), ellipsoid to elongate, rarely broadly ellipsoid, inamyloid, colorless, hyaline, thin-walled, smooth; apiculus small. Lamellar edge appearing as a sterile strip up to 150 μm wide in side view, predominantly composed of inflated cells, ovoid to subglobose or short ellipsoid (10–35 × 8–11 μm), single and terminal, or often in chains of 2–3, nearly colorless and hyaline, or occasionally with brownish vacuolar pigments; filamentous hyphae fairly abundant to scattered, 3–7 μm wide, colorless or with brownish vacuolar pigments. Pileipellis 95–140 μm thick; upper layer (25–50 μm thick) strongly to moderately gelatinized, composed of ± radially and moderately compactly arranged, thin-walled, colorless to nearly colorless, strongly to moderately gelatinized filamentous hyphae 2–5 μm wide; vascular hyphae rare, 2–4 μm wide; lower layer (70–90 μm thick) composed of radially and compactly arranged, filamentous hyphae 3–7 μm wide, sometimes with intercalary segments inflated to 12 μm wide, usually with dark yellowish brown, vacuolar pigments; vascular hyphae fairly rare, 2–6 μm wide. Volval remnants on pileus composed of ± irregularly arranged elements: inflated cells very abundant (to locally dominant), subglobose (20–50 × 10–20 μm) or ovoid to broadly clavate (20–35 × 18–30 μm), single and terminal, or in chains of 2–3 and then terminal, thin to slightly thick-walled (up to 1 μm thick), usually colorless and hyaline, occasionally with brownish vacuolar pigments; filamentous hyphae fairly abundant, 4–8 μm wide, frequently branching, often anastomosing, thin-walled, colorless, hyaline or occasionally with brownish vacuolar pigments; vascular hyphae rare, 4–5 μm wide. Volval remnants on stipe base similar to those on the pileus, but with more abundant filamentous hyphae. Stipe trama composed primarily of longitudinally arranged, clavate to long clavate, terminal cells, 78–300 × 15–30 μm; filamentous hyphae 3–8 μm wide, scattered (in interior) or fairly abundant to abundant (on stipe surface), colorless and hyaline (in interior), occasionally with brownish to brown vacuolar pigments (on stipe surface); vascular hyphae 5–12 μm wide, rare to locally conspicuous. Annulus predominantly composed of thin-walled, colorless and hyaline, frequently septate filamentous hyphae 2–6 μm wide; inflated cells and vascular hyphae rare.

Habit and habitat:—on soil under trees of Fagaceae and/or Pinaceae .

Distribution:—Currently known from Hunan and Jiangxi provinces, Central China.

Additional specimen examined:— CHINA. Jiangxi Province: Jiujiang County, Lushan Scenic Spot, on the ground in a mixed forest dominated by members of Fagaceae and Pinaceae , 29°32′50.59″ N 115°57′46.41″E, elev. 950–994m, 21 July 2017, G. Wu 2185 (HKAS100640, GenBank Acc. No.: nrLSU = MF952721, ITS = MF928093).

Figures

FIGURE 3. Basidiomata of Amanita cingulata. a. HKAS 75600 (holotype!) photos by Qin Cai. b. c. d. HKAS 100640, Photos by Gang Wu. Bars: a–d = 2 cm.

FIGURE 4. Microscopic features of Amanita cingulata (HKAS 75600, holotype). a. Basidiospores. b. Radial section of annulus c. Hymenium and subhymenium. d. Crushed volval remnants on the pileus (vascular hyphae are shaded). Drawings by Jian-Wei Liu. Bars = 10 μm.