Fitzroyomyces yunnanensis L. Lu, K.D. Hyde & Tibpromma, 2021

Lu, Li, Tibpromma, Saowaluck, Karunarathna, Samantha C., Thiyagaraja, Vinodhini, Xu, Jianchu, Jayawardena, Ruvishika S., Lumyong, Saisamorn & Hyde, Kevin D., 2021, Taxonomic and phylogenic appraisal of a novel species and a new record of Stictidaceae from coffee in Yunnan Province, China, Phytotaxa 528 (2), pp. 111-124 : 118

Fitzroyomyces yunnanensis L. Lu, K.D. Hyde & Tibpromma , sp. nov. ( Fig. 2 )

Index Fungorum number: IF 558662; Facesoffungi number: FoF 09896

Holotype:— CHINA. Yunnan Province: Xishuangbanna , on dead wood of coffee, 24 November 2020, Saowaluck Tibpromma, XSBN-C5 ( HKAS 113186 , holotype); ex-type living culture KUMCC 21-0002 .

Saprobic on dead wood of coffee. Sexual morph: Apothecia 550–1000 × 230–370 μm (x̅ = 800 × 270 μm, n = 10), immersed at first, opening by entire pore at maturity, subglobose. Disc deeply cupulate, pale creamy, with whitepruinose margin. Exciple 25–50 μm wide (x̅ = 38 μm, n = 10), thick-walled, comprising hyaline cells of textura intricata, inner layer composed of hyaline cells of textura angularis. Hamathecium comprising asci and paraphyses. Paraphyses hyaline, 1–2 µm wide (x̅ = 1.8 μm), numerous, long filiform, unbranched, aseptate. Asci 100–140 × 5–10 μm (x̅ = 112 × 8 μm, n = 10), unitunicate, 6–8-spored, cylindrical to ovoid, broadest at middle, rounded apex and short stalked. Ascospores 80–120 × 2–3 μm (x̅ = 88 × 2.7 µm, n = 10), overlapping uni- to bi-seriate, fusiform, 12–18- septate, hyaline, not easily breaking into different sizes, smooth-walled, guttulate, without a mucilaginous sheath. Asexual morph: Undetermined.

Culture characteristics:— Colonies on PDA reaching 35 mm diam, after 4 weeks at room temperature (20–25 ℃). Culture from above, convex with surface, dry, rough, white to pale brown, circular, towards the edge with irregular white aerial mycelia, spreading, slightly covered; reverse brown to yellowish, with radiating white mycelia.

Etymology:— The specific epithet “ yunnanensis ” refers to Yunnan Province where the holotype was collected.

Notes:— ITS blast result showed that Fitzroyomyces yunnanensis is 89% similar to F. cyperacearum ( MK499349 ). The results of LSU blast showed that our strain is 96% similar to Stictis sp. ( MH260319 ), while mtSSU results showed it to be 90% similar to Carestiella socia ( AY661678 ). Based on phylogenetic analyses, the new strain clustered with Fitzroyomyces and formed a well-separated clade with 100% ML, 1.00 BYPP boostrap support with the extant species of Fitzroyomyces . Fitzroyomyces yunnanensis differs from extant species of Fitzroyomyces in its larger apothecia, with a clear border between outer and inner layers in the exciple, and shorter asci and ascospores. This is the first Fitzroyomyces species reported from coffee. A synopsis of characters for sexual taxa in Fitzroyomyces is provided ( Table 2 ).

Figures

FIGURE 2. Fitzroyomyces yunnanensis (HKAS 113186, holotype). a, b. Ascomata on coffee wood. c, e. Exciple. d. Vertical section of apothecium. f. Paraphyses. g. Ascus. h–j. Ascospores. k. Germinating ascospore. l, m. Colonies on PDA media. Scale bars: c = 10 µm, d = 500 µm, e–k = 30 µm.