Hirschmanniella shamimi Ahmad, 1972

Zhuo, Kan, Liao, Jinling, Cui, Ruqiang & Li, Yuzhong, 2009, First record of female intersex in Hirschmanniella shamimi Ahmad, 1972 (Nematoda: Pratylenchidae), with a checklist of intersexes in plant nematodes, Zootaxa 1973, pp. 61-68 : 62

Hirschmanniella shamimi Ahmad, 1972 , female intersexes

( Figs. 1 , 2 )

Type material and locality: Slide HSI 1 and HSI 2 deposited in Laboratory of Plant Nematology, South China Agricultural University, Guangzhou, China. Sample was collected in May 2006 from soil samples around rice roots, in Guangzhou, Guangdong Province, China.

Measurements. See Table 1 .

Gubernaculum μm 7.6 7.1 – 8.2±0.2 – –

(7.6–9.0) (7–9) Description. Body slightly curved ventrally when heat relaxed. Lip region rounded, with 5 annuli. Stylet knobs rounded. Pharyngeal gland lobes long, ventrally overlapping intestine, 249 μm and 252 μm from anterior end for HSI1 and HSI2 respectively. Excretory pore posterior to level of esophago-intestinal valve. Lateral fields with four lines, not areolated near mid-body. Tail elongate conoid, terminus with a fine mucro and a ventral notch. The female reproductive system developed. Vulva well developed, in shape similar with the normal female vulva. Gonads didelphic, with outstretched ovaries, anterior genital branch 353 μm and 267μm long for HSI1 and HSI2 respectively, posterior genital branch 383 μm and 305 μm long for HS1 and HSI2 respectively. Spermatheca oval and filled with sperm cells. The male reproductive system reduced. Testis not detected. Spicules arcuate, well developed, similar to normal male. Gubernaculum simple, thin, slightly arcuate. Bursa extending 42.8 μm along ventral body in HSI2, but absent in HSI1.

Figures

FIGURE 1. Hirschmanniella shamimi intersex. a, entire body (HSI 1); b, posterior half (HSI 2)

FIGURE 2. Hirschmanniella shamimi intersex. a, anterior region (HSI 1); b, posterior half with vulva and spicule (HSI 1), vulva and spicule showed by an arrow respectively; c, anterior genital branch of gonads (HSI 1); d, tail (HSI 1), showing spicule without bursa; e, anterior genital branch of gonads (HSI 2); f, tail (HSI 2), showing spicule and bursa.

Tables

TABLE 1. Biometrics of female intersexes in Hirschmanniella shamimi compared with normal females and males.

n	1	1	20	10	17	10
L (μm)	1429	1275	1450±16.3 (1320–1540)	1350±24.9 (1260–1430)	1270 (1190–1360)	1240 (1200–1360)
a	56.7	56.2	53.6±1.0 (47.3–60.0)	54.9±0.9 (50.0–58.2)	51 (40–59)	52 (43–59)
b	11.1	11.2	11.7±0.1 (11.0–12.8)	12.1±0.2 (10.9–13.2)	11.0 (10.1–13.8)	12.0 (10.5–14.0)
b’	5.7	5.1	5.1±0.2 (3.9–6.6)	5.1±0.1 (4.7–5.6)	4.7 (3.8–5.6)	4.8 (4.4–5.1)
c	18.3	16.9	15.9±0.2 (15.0–18.0)	18.1±0.4 (16.1–19.7)	20 (17–23)	19 (17–21)
c’	4.1	5.1	4.9±0.1 (4.5–5.6)	5.2±0.1 (4.8–5.7)	– (3.5–5.0)	–
V%	53.1	52.4	52.4±0.3 (51.0–55.2)	–	54 (51–56)	–
Stylet μm	20.2	17.6	18.0±0.1 (17.5–18.5)	17.8±0.2 (17.0–18.5)	17 (16–19)	–
Spicule μm	22.7	21.4	–	23.6±0.3 (22.1–24.8)	–	– (22–25)