Strongyloides genotypes (Barratt and Sapp, 2020)

Richins, Travis, Sapp, Sarah G. H., Ketzis, Jennifer K., Willingham, Arve Lee, Mukaratirwa, Samson, Qvarnstrom, Yvonne & Barratt, Joel L. N., 2023, Genetic characterization of Strongyloides fuelleborni infecting free-roaming African vervets (Chlorocebus aethiops sabaeus) on the Caribbean island of St. Kitts, International Journal for Parasitology: Parasites and Wildlife 20, pp. 153-161 : 155

2.6. Reference dataset of published Strongyloides genotypes

We used 128 S. fuelleborni genotypes compiled as part of previous work ( Barratt et al., 2019a; Barratt and Sapp, 2020), plus 18 from an undefined Strongyloides species identified in Bornean slow lorises ( Frias et al., 2018). Our dataset included 24 cox1 sequences and HVR-IV sequences from Thai isolates of S. fuelleborni : one from a human and 23 from pig-tailed macaques ( Macaca nemestrina ) ( Janwan et al., 2020). We also included 39 S. fuelleborni cox1 sequences generated by Ko et al. (2023) from a range of Asian non-human primates including rhesus macaques from Myanmar ( Macaca mulatta ), Japanese macaques ( Macaca fuscata ), and sequences from four primate species housed in zoological parks in Japan; siamang ( Symphalangus syndactylus ), red-shanked douc ( Pygathrix nemaeus ), Francois’ langur ( Trachypithecus francoisi ), and Bornean Orangutan ( Pongo pygmaeus ) ( Ko et al., 2023). To serve as an outgroup for our phylogenetic analysis, we sequenced our S. stercoralis PCR positive control amplicons in the same Illumina library as the vervet samples, and included 28 previously published S. stercoralis genotypes ( Barratt and Sapp, 2020) , plus a cox1 sequence extracted from the mitochondrial genome of S. stercoralis reference strain PV001 (GenBank [GB] - accession: NC_028624.1). A detailed overview of all specimens within this dataset, including information on hosts, genotype, and GenBank accession numbers, is provided in File S1, Tab A.