Amanita manicata (Berk. & Broome) Pegler, Kew Bull., Addit. Ser.

Liu, Yuan S., Kumla, Jaturong, Suwannarach, Nakarin, Sysouphanthong, Phongeun & Lumyong, Saisamorn, 2022, Three species of Amanita section Lepidella (Amanitaceae, Agaricales) from northern Thailand, Phytotaxa 570 (1), pp. 16-28 : 24-25

Amanita manicata (Berk. & Broome) Pegler, Kew Bull., Addit. Ser. 12: 216 (1986). Figure 1d

Habitat: Solitary to scattered in black sand at berm of beach, on soil in grass or forest, in composted woodchips, April to October.

Distribution: Known from Dominican Republic, New Zealand, Sri Lanka, Thailand, and the USA (Hawaiian Islands) ( Petch 1910; Pegler 1986; Hemmes & Desjardin 2008).

Specimens examined: THAILAND. Chiang Rai Province: Mae Salong District, N 20°12’21”, E 99°41’12”, alt. 570 m, 1Aug 2019, Yuan S. Liu, STO-2019-347 (SDBR-STO-2019-347, GenBank accession no.: nrLSU = OM 980706 , ITS = ON000813 , RPB2 = ON007283 , and TEF1 -α = ON007301 ); Chiang Mai Province: Sansai District , N 19°05’17”, E 98°58’43”, alt. 410 m, 6 Aug 2019, Yuan S. Liu, STO-2019-390 (SDBR-STO-2019-390, GenBank accession no.: nrLSU = ON692698 , ITS = ON692927 ); Chiang Mai Province: Mueang District , N 18°52’05”, E 98°56’42”, alt. 354 m, 25 Jun 2020, Yuan S. Liu, STO-2020-34 (SDBR-STO-2020-34, GenBank accession no.: nrLSU = OM 980707 , ITS = ON000814 , RPB2 = ON007284 , and TEF1 -α = ON007302 ); Yuan S. Liu, STO-2020-36 (SDBR-STO-2020-36, GenBank accession no.: nrLSU = OM 980708 , ITS = ON000815 , RPB2 = ON007285 , and TEF1 -α = ON007285 ) .

Remarks: Amanita manicata is characterized by its yellowish brown to pale tawny brown pileus covering with floccoso-verrucose to felty squamules; margin appendiculates with large floccose fragments which hang down up to 2 cm; the cylindrical stipe covering with tawny brown floccoso-squamose which becomes more intense and thicker as upwards; the cream to whitish or pinkish tint lamellae; the subglobose and amyloid basidiospores (7.0–8.5 × 5.5–8.0 μm), as well as the present clamps. In our phylogenetic analysis, A. manicata closed to A. flavofloccosa Nagas. & Hongo , A. foetidissima Reid & Eicker and A. nauseosa (Wakef.) Reid and they formed a sister group ( Figure 2 ). Meanwhile, these four species share the similar appearance and color tone of basidioma, as well as the intense and unpleasant odor, which make people easily confuse for these species in the field. However, A. flavofloccosa can be distinguished from A. manicata by its yellow-brown to orange-yellow pileus and white, pinkish to yellowish lamellae ( Cui et al. 2018). Amanita foetidissima , originally reported from South Africa ( Reid & Eicker 1991), has a pale creamy buff to buff pileus, oval to subglobose (7.0–9.0 × 6.2–8.0 μm), to broadly ellipsoid (9.0–10.0 × 6.0–6.2 μm) basidiospores, as well as the larger basidia (50.0–52.0 × 9.0–9.5 μm). Although A. nauseosa is very similar to A. manicata , it has a buff, or reddish-tinged to pale ochraceous buff surface and the greater basidiospores (7.0–9.0 × 6.5–8.0 μm) ( Bas 1969).

Figures

FIGURE 1. Fresh basidiomata of the studied Amanita species and location of the sampling sites. a–b: A. albicarnosa (CMUB-39971, holotype). c: A. aureofloccosa (SDBR-STO-2020-PS39). d: A. manicata (SDBR-STO-2020-34). e: Location of the sampling sites. Scale bars: a–b=3 cm, c=8 cm, d=5 cm.

FIGURE 2. RAxML tree based on nrLSU-ITS dataset. Bootstrap values (BS) for ML ≥ 50% and posterior probabilities (PP) for BI ≥ 0.95 are placed above or below the branches respectively. Newly generated sequences are indicated in red. The tree is rooted with Limacella bangladeshana (CMU-JK0146, CMU-NK0356 & HKAS 75316).