Melanaphis donacis, KF

Skvarla, Michael J., Miller, Gary L., Bauchan, Gary, Lewis, Matthew, Foottit, Robert & Maw, Eric, 2018, Taxonomy and Natural History of Cattail Aphids, Rhopalosiphum enigmae (Hemiptera: Aphidomorpha: Aphididae), Including a New Synonymy and Notes on Ant and Parasitoid Associates of Rhopalosiphum, Insect Systematics and Diversity 2 (2018), No. 2, pp. 1-14 : 6-8

M. donacis KF 639526.1

R. enigmae AB Vermilion River Vermilion 53°21.690′N, 110°52.200′W Typha latifolia 12-June-2009 E. Maw, R.G. CNC#HEM063600 GU668768 Provincial Park Foottit

R. enigmae AB Bonnyville Truman, Hwy 55 54°28.074′N, 111°11.160′W Typha latifolia 14-July-2009 E. Maw, R.G. CNC#HEM063643 GU668766 at Sand River Foottit

R. enigmae ON Ottawa Mer Bleu Cons. 45°24.252′N, 75°33.882′W Typha latifolia 1-August-2008 E. Maw et al. CNC#HEM061388 KR038471.1 Area

R. enigmae ON Ottawa Ottawa 45°23.652′N, 75°42.198′W Typha latifolia 25-September-2009 G. Miller, CNC#HEM064177 GU668786 E. Maw

R. enigmae DE Kent Felton 39°00.730′N, 75°35.746′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-006 MF123452 & G. Miller

R. enigmae DE Sussex Georgetown 38°44.570′N, 75°25.602′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-001 MF123417 & G. Miller

R. enigmae DE Sussex Selbyville 38°28.778′N, 75°14.093′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-004 & G. Miller

R. enigmae DE Sussex Selbyville 38°28.778′N, 75°14.093′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-005 MF123422 & G. Miller

R. enigmae FL Collier Immokalee Suction trap 10–17-February-2016 S. Halbert E2016-536-2 MF123420

R. enigmae FL Collier Immokalee Suction trap 24-February-2016 – S. Halbert E2016-731-1 MF123449 2-March-2016

R. enigmae FL Polk Lake Alfred 28°08.869′N, 81°44.301′W Typha 25-September-2016 M. J. Skvarla MS 16-0925-001 MF123434

R. enigmae FL Polk Winter Haven 28°03.350′N, 81°44.050′W suction trap 11–18-February-2016 S. Halbert E2016-535-2 MF123437

R. enigmae FL St. Johns St. Augustine 29°54.959′N, 81°24.846′W Typha 22-September-2016 M. J. Skvarla MS 16-0922-001 MF123448

R. enigmae FL Walton Point Washington 30°22.185′N, 86°06.391′W Typha 25-April-2016 K. E. Schnepp KS 16-0425-001 MF123433

R. enigmae GA McIntosh Darien 31°22.536′N, 81°25.862′W Typha 22-September-2016 M. J. Skvarla MS 16-0922-003 MF123435

R. enigmae MD Anne Arundel Russett 39° 06.267′N, 76°48.004′W Typha 10-May-2016 M. J. Skvarla MS 16-0510-001 MF123421

R. enigmae MD Anne Arundel Russett 39° 06.267′N, 76°48.004′W Typha 21-April-2016 M. J. Skvarla MS 16-0421-001 MF123425

R. enigmae MD Anne Arundel Russett 39° 06.267′N, 76°48.004′W Typha 19-April-2017 M. J. Skvarla MS 16-0419-001

R. enigmae MD Dorchester Linkwood 38°33.105′N, 75°57.521′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-009 MF123453 , MF123430 & G. Miller

R. enigmae MD Frederick Frederick 39°22.860′N, 77°24.410′W Typha 15-June-2016 M. J. Skvarla MS 16-0615-001 MF123450 , MF123447

R. enigmae MD Queen Anne’s Chester 38°57.294′N, 76°17.981′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-002 MF123432 & G. Miller

R. enigmae MD Queen Anne’s Grasonville 38°57.850′N, 76°13.253′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-008 MF123424 , MF123439 & G. Miller

R. enigmae MD Queen Anne’s Grasonville 38°57.850′N, 76°13.253′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-010 & G. Miller

R. enigmae MD Talbot Easton 38°49.014′N, 76°03.686′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-003 MF123418 , MF123445 & G. Miller

R. enigmae MD Washington Hancock 39°42.142′N, 78°11.429′W Typha 15-July-2016 M. J. Skvarla MS 16-0715-002

R. enigmae MD Washington Hancock 39°42.142′N, 78°11.429′W Typha 15-July-2016 M. J. Skvarla MS 16-0715-003

R. enigmae MD Wicomico Salisbury 38°22.178′N, 75°32.160′W Typha 28-July-2016 M. J. Skvarla MS 16-0728-007 MF123431 & G. Miller

member of the subtribe Rhopalosiphina , is thought to be closely related to Rhopalosiphum and was used to root the phylogenetic tree. The COI sequence for M. donacis was obtained from GenBank ( Table 3). R. musae and R. nymphaeae were included in order to help root the phylogenetic tree, and to determine the percent difference in COI between species and thus provide a baseline comparison for the percent difference in COI between R. enigmae and R. laconae . In addition to COI sequences produced de novo and available through GenBank, seven R. enigmae , one R. nymphaeae , and one R. musae were available through the Barcode of Life Database and included in analyses ( Table 3).

Bayesian analyses were performed with MrBayes (3.2.6) using the Extreme Science and Engineering Discovery Environment (XSEDE) infrastructure on the Cipres Portal ( Miller et al. 2010). Each analysis consisted of four simultaneous runs, each with four chains sampling every 1,000 generations for 1.11 million generations, under a GTR+I+Γ model of molecular evolution. The analysis was automatically ended when the split frequencies fell below 0.01; 25% of the trees were discarded as burn-in. The resulting majority-rule consensus trees were viewed with Dendroscope 3 (v. 3.5.7) ( Huson and Scornavacca 2012); tree image files were then exported in PDF format and edited for final figures in Adobe Illustrator CS6 (Adobe Systems, San Jose, CA).

Percent difference in COI was determined by comparing sequence data for pairs of individuals in BioEdit ( Hall 1999) using the ‘calculate identity/similarity for two sequences’ function. At least one such pair comparison was made within and between each clade and additional within-clade comparisons were made when multiple morphologies existed within a single clade, such that 19 pair comparisons were made within the larger R. enigmae + R. laconae clade, 1 pair comparison was made within the R. musae clade, 2 pair comparisons were made within the R. nymphaeae clade, and 1 pair comparison was made between R. enigmae + R. laconae and each of the outgroup clades.