Melanaphis donacis, KF

Skvarla, Michael J., Miller, Gary L., Bauchan, Gary, Lewis, Matthew, Foottit, Robert & Maw, Eric, 2018, Taxonomy and Natural History of Cattail Aphids, Rhopalosiphum enigmae (Hemiptera: Aphidomorpha: Aphididae), Including a New Synonymy and Notes on Ant and Parasitoid Associates of Rhopalosiphum, Insect Systematics and Diversity 2 (2018), No. 2, pp. 1-14: 6-8

publication ID

http://doi.org/ 10.1093/isd/ixy001

publication LSID

lsid:zoobank.org:pub:DE305539-03BD-473E-AA5B-87B079E61E0E

persistent identifier

http://treatment.plazi.org/id/03D29D27-E349-FF82-6DA2-FD9B2016DF5D

treatment provided by

Felipe

scientific name

Melanaphis donacis
status

 

M. donacis KF   639526.1

R. enigmae AB Vermilion River Vermilion   53°21.690′N, 110°52.200′W Typha latifolia   12-June-2009 E. Maw, R.G. CNC#HEM063600 GU668768 View Materials Provincial Park Foottit

R. enigmae AB Bonnyville Truman, Hwy   55 54°28.074′N, 111°11.160′W Typha latifolia   14-July-2009 E. Maw, R.G. CNC#HEM063643 GU668766 View Materials at Sand River Foottit

R. enigmae ON Ottawa Mer Bleu Cons.   45°24.252′N, 75°33.882′W Typha latifolia   1-August-2008 E. Maw et al. CNC#HEM061388 KR038471.1 Area

R. enigmae ON Ottawa Ottawa   45°23.652′N, 75°42.198′W Typha latifolia   25-September-2009 G. Miller, CNC#HEM064177 GU668786 View Materials E. Maw

R. enigmae DE   Kent Felton 39°00.730′N, 75°35.746′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-006 MF123452 View Materials & G. Miller

R. enigmae DE Sussex Georgetown   38°44.570′N, 75°25.602′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-001 MF123417 View Materials & G. Miller

R. enigmae DE Sussex Selbyville   38°28.778′N, 75°14.093′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-004 & G. Miller

R. enigmae DE Sussex Selbyville   38°28.778′N, 75°14.093′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-005 MF123422 View Materials & G. Miller

R. enigmae FL Collier Immokalee Suction   trap 10–17-February-2016 S. Halbert E2016-536-2 MF123420 View Materials

R. enigmae FL Collier Immokalee Suction   trap 24-February-2016 – S. Halbert E2016-731-1 MF123449 View Materials 2-March-2016

R. enigmae FL Polk Lake Alfred   28°08.869′N, 81°44.301′W Typha   25-September-2016 M. J. Skvarla MS 16-0925-001 MF123434 View Materials

R. enigmae FL Polk Winter Haven   28°03.350′N, 81°44.050′W suction trap 11–18-February-2016 S. Halbert E2016-535-2 MF123437 View Materials

R. enigmae FL St. Johns St. Augustine   29°54.959′N, 81°24.846′W Typha   22-September-2016 M. J. Skvarla MS 16-0922-001 MF123448 View Materials

R. enigmae FL Walton Point   Washington 30°22.185′N, 86°06.391′W Typha   25-April-2016 K. E. Schnepp KS 16-0425-001 MF123433 View Materials

R. enigmae GA McIntosh Darien   31°22.536′N, 81°25.862′W Typha   22-September-2016 M. J. Skvarla MS 16-0922-003 MF123435 View Materials

R. enigmae MD Anne Arundel Russett   39° 06.267′N, 76°48.004′W Typha   10-May-2016 M. J. Skvarla MS 16-0510-001 MF123421 View Materials

R. enigmae MD Anne Arundel Russett   39° 06.267′N, 76°48.004′W Typha   21-April-2016 M. J. Skvarla MS 16-0421-001 MF123425 View Materials

R. enigmae MD Anne Arundel Russett   39° 06.267′N, 76°48.004′W Typha   19-April-2017 M. J. Skvarla MS 16-0419-001

R. enigmae MD Dorchester Linkwood   38°33.105′N, 75°57.521′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-009 MF123453 View Materials , MF123430 View Materials & G. Miller

R. enigmae MD Frederick Frederick   39°22.860′N, 77°24.410′W Typha   15-June-2016 M. J. Skvarla MS 16-0615-001 MF123450 View Materials , MF123447 View Materials

R. enigmae MD   Queen Anne’s Chester 38°57.294′N, 76°17.981′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-002 MF123432 View Materials & G. Miller

R. enigmae MD   Queen Anne’s Grasonville 38°57.850′N, 76°13.253′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-008 MF123424 View Materials , MF123439 View Materials & G. Miller

R. enigmae MD   Queen Anne’s Grasonville 38°57.850′N, 76°13.253′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-010 & G. Miller

R. enigmae MD Talbot Easton   38°49.014′N, 76°03.686′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-003 MF123418 View Materials , MF123445 View Materials & G. Miller

R. enigmae MD   Washington Hancock 39°42.142′N, 78°11.429′W Typha   15-July-2016 M. J. Skvarla MS 16-0715-002

R. enigmae MD   Washington Hancock 39°42.142′N, 78°11.429′W Typha   15-July-2016 M. J. Skvarla MS 16-0715-003

R. enigmae MD Wicomico Salisbury   38°22.178′N, 75°32.160′W Typha   28-July-2016 M. J. Skvarla MS 16-0728-007 MF123431 View Materials & G. Miller

member of the subtribe Rhopalosiphina   , is thought to be closely related to Rhopalosiphum   and was used to root the phylogenetic tree. The COI sequence for M. donacis   was obtained from GenBank ( Table 3). R. musae   and R. nymphaeae   were included in order to help root the phylogenetic tree, and to determine the percent difference in COI between species and thus provide a baseline comparison for the percent difference in COI between R. enigmae   and R. laconae   . In addition to COI sequences produced de novo and available through GenBank, seven R. enigmae   , one R. nymphaeae   , and one R. musae   were available through the Barcode of Life Database and included in analyses ( Table 3).

Bayesian analyses were performed with MrBayes (3.2.6) using the Extreme Science and Engineering Discovery Environment (XSEDE) infrastructure on the Cipres Portal ( Miller et al. 2010). Each analysis consisted of four simultaneous runs, each with four chains sampling every 1,000 generations for 1.11 million generations, under a GTR+I+Γ model of molecular evolution. The analysis was automatically ended when the split frequencies fell below 0.01; 25% of the trees were discarded as burn-in. The resulting majority-rule consensus trees were viewed with Dendroscope 3 (v. 3.5.7) ( Huson and Scornavacca 2012); tree image files were then exported in PDF format and edited for final figures in Adobe Illustrator CS6 (Adobe Systems, San Jose, CA).

Percent difference in COI was determined by comparing sequence data for pairs of individuals in BioEdit ( Hall 1999) using the ‘calculate identity/similarity for two sequences’ function. At least one such pair comparison was made within and between each clade and additional within-clade comparisons were made when multiple morphologies existed within a single clade, such that 19 pair comparisons were made within the larger R. enigmae   + R. laconae   clade, 1 pair comparison was made within the R. musae   clade, 2 pair comparisons were made within the R. nymphaeae   clade, and 1 pair comparison was made between R. enigmae   + R. laconae   and each of the outgroup clades.