Beauveria bassiana, (Bals.-Criv.) Vuill.

Eliwa, Duaa, Albadry, Mohamed A., Ibrahim, Abdel-Rahim S., Kabbash, Amal, Meepagala, Kumudini, Khan, Ikhlas A., El-Aasr, Mona & Ross, Samir A., 2021, Biotransformation of papaverine and in silico docking studies of the metabolites on human phosphodiesterase 10 a, Phytochemistry (112598) 183, pp. 1-12 : 9

3.7. Microbial transformation using Beauveria bassiana 22864

Biotransformation of papaverine using Beauveria bassiana 22864 afforded to two metabolites: 5 and 6. The residue (1 g) was dissolved in 30 ml of methanol/dichloromethane mixture (1:1) and adsorbed onto 1 g Celite and dried. The adsorbed sample was placed onto a glass column (110 × 2.5 cm) packed with silica after making a slurry in dichloromethane. The column was gradiently eluted with dichloromethane: methanol (99:1, 95:5,94:6) and 10 ml fractions were collected. Fractions 50–119 were pooled together to give mixture of the two metabolites (80 mg), as determined by TLC. The two metabolites were separated by preparative silica gel 60 PR-18 plates (10 × 20 cm, 250 μm) (methanol: water, 70:30). 5 mg of the sample added in each plate. The bands were located using UV lamp then scratched and extracted using 100% methanol then evaporated, yielding to pure 5 in the form of white powder (5 mg, R f 0.69 S 3) and pure 6 white powder (6 mg, R f 0.61 S 3).