Ablabesmyia (Karelia) makarchenkoi, Niitsuma, Hiromi, 2013

Niitsuma, Hiromi, 2013, Revision of the Japanese Ablabesmyia (Diptera: Chironomidae: Tanypodinae), with descriptions of three new species, Zootaxa 3664 (4), pp. 479-504 : 496-500

Ablabesmyia (Karelia) makarchenkoi sp. n.

( Figs 52–63 )

Type material. Holotype male with pupal exuviae (SUM-IC-T0476), JAPAN: Fukushima, Iwaki, Yaguki, 15.viii.2009 (adult emerged 29.viii.2009). Paratypes. Same data as holotype except 3 females with 3 pupal exuviae (SUM-IC-T0478–T0480, adults emerged 19–30.viii.2009); same data as holotype except 1 female with pupal and larval exuviae (SUM-IC-T0481), 15.vii.2012 (adult emerged 3.viii.2012); Gifu, Mino, Nagara River, 1 female with pupal exuviae (SUM-IC-T0490), 12.ix.2009 (adult emerged 18.ix.2009).

Etymology. Named in honour of Dr. Eugenyi A. Makarchenko in recognition of his contribution to the study of Russian chironomids.

Description. Male (n = 1). Body length 3.2 mm.

Colouration. Thorax brown with dark scutal vittae, anepisternum II, preepisternum and postnotum. Abdominal tergites I–V pale brown; tergites VI–VIII mostly dark brown; hypopygium yellow. Wing ( Fig. 52 ) darkened on humeral cross-vein, RM, FCu, apex of R4+5, and areas along C between apices of R1 and R3, and above RM; membrane with dark markings; dark spot in middle of cell r4+5 distad to spot in middle of cell m1+2; distal marking in anal cell hourglass-shaped. Leg segments white with dark bands; subbasal and median bands on foretibia located 0.77 and 0.41, respectively, from apex of tibia.

Head. Temporals 29, multiserial except uniserial postorbitals. AR 1.7. Clypeus trapezoid with 34 setae. Lengths of palpomeres 1–5 (μm): 60, 100, 193, 215, 335.

Thorax. Aps 13; Ac 41, biserial between median scutal vittae, diverging posteriorly; Dc 17, uniserial, becoming biserial anteriorly; H 15; Pa 28; Su 1; Scts 48.

Wing. Length 2.3 mm. Squama with 38 setae. VR 0.83.

Legs. Spur on foretibia 60 μm long, with 12 lateral teeth. Spurs on mid tibia 70 and 58 μm long, with 10 and 7 lateral teeth, respectively. Spurs on hind tibia 75 and 58 μm long, with 6 and 5 lateral teeth, respectively; hind tibial comb consisting of 5 bristles. All legs with subapical pseudospurs on ta1–3; claws pointed apically, each with 2 long and 3–4 short basoventral spines. Lengths and proportions of leg segments as in Table 5 .

Hypopygium ( Fig. 53 ). Tergite IX with 4 lateral setae. Gonocoxite 138 μm long; dorsal lobe 30 μm long, with apical brush of setae, directed laterally; blade 45 μm long, with ridged surface and several separate points apically; lateral lobe 55 μm long, broad and apically pointed; lateral filaments not evident ( Fig. 54 ). Gonostylus 173 μm long, with apically acute megaseta.

Female (n = 5). Body length 2.3–2.6, 2.5 mm.

Colouration. Similar to male. Occasionally abdominal tergites II–VII each with T-shaped dark marking and tergite VIII extensively dark. Subbasal and median bands on foretibia located 0.78–0.79 and 0.39–0.43, respectively, from apex of tibia. Seminal capsule brown except pale neck.

Head. Temporals 26–40, 33. Antenna with terminal flagellomere almost as long as or somewhat longer than preceding 3 flagellomeres together; AR 0.29–0.32. Clypeus with 35–60, 45 setae. Lengths of palpomeres 1–5 (μm): 60–75, 68 (4); 88–100, 93 (4); 160–190, 180 (4); 195–213, 206 (4); 310–330, 323 (4). Pm4 1.1–1.3 times as long as Pm3; Pm5 1.5–1.7 times as long as Pm4.

Thorax. Aps 13–22, 18; Ac 55–62, 59 (4); Dc 23–25, 24; H 18–24, 21; Pa 25–33, 29; Su 1; Scts 45–52, 49.

Wing. Length 2.0–2.3, 2.1 mm. Squama with 42–48, 45 (4) setae. VR 0.84–0.89.

Legs. Spur on foretibia 53–55, 54 μm long, with 8–10, 9 lateral teeth. Spurs on mid tibia 65–75, 68 and 50–55, 53 μm long, with 8–10, 9 (4) and 6–8, 7 lateral teeth, respectively; inner spur 1.2–1.4 times as long as outer spur. Spurs on hind tibia 68–75, 72 and 50–55, 53 μm long, with 6–7 (2) and 4–6, 5 (4) lateral teeth, respectively; inner spur 1.3–1.5 times as long as outer spur; hind tibial comb consisting of 5 bristles. All legs with subapical pseudospurs on ta1–3; hind leg with 4–6, 5 (3) sensilla chaetica on basal 0.48–0.59 of ta1. Lengths and proportions of leg segments as in Table 5 .

Genitalia ( Fig. 55 ). Gonocoxapodeme VIII well developed. Gonapophysis VIII somewhat tongue-shaped. Gonapophysis IX well developed; notum 150–160, 153 (4) μm long, and almost twice as long as ramus. Labium without microtrichia. Seminal capsule oval, 58–63, 60 (4) μm long, and 50–58, 53 (4) μm wide. Segment X reduced with 4–7, 5 (4) setae. Postgenital plate rounded apically.

Pupa (n = 6). Body length 4.5–5.0, 4.7 mm.

Colouration. Exuviae brown. Wing sheath entirely brown, slightly darkened along margin and veins. Abdominal tergites II–VIII entirely brown, with no distinct pattern ( Fig. 56 ).

Cephalothorax. Thoracic horn 355–420, 388 μm long and 1.9–2.5 times as long as broadest width; apex of aeropyle tube club-shaped ( Fig. 57 ); reticulation weak as in A. (A.) monilis . Thoracic comb composed of 9–11, 10 teeth rounded apically.

Abdomen ( Fig. 58 ). Tergal shagreen ( Fig. 59 ) consisting of slightly arched, transverse rows of blunt spinules. LS1-seta on A.VII located 0.46–0.50 from anterior margin, and on A.VIII located 0.31–0.36. Anal lobe 425–445, 434 μm long and 2.0–2.3 times as long as wide; anterior and posterior anal macrosetae located 0.44–0.53 and 0.56– 0.63, respectively, from anterior margin; male genital sac 0.71 (1) as long as anal lobe.

Fourth instar larva (n = 1).

Colouration. Head yellow with dark postoccipital margin; body entirely yellow when live.

Head. Length 1111 μm; cephalic index 0.50. Lines S9-VP and S10-VP making acute angle ( Fig. 60 ). Lengths of antennal segments 1–4 (μm): 540, 120, 6, 5. AR 4.1. First antennal segment 18.0 times as long as basal width, with ring organ located 0.57 from base; blade 128 μm long, reaching middle of 3rd segment; accessory blade 125 μm long. Second antennal segment 19.2 times as long as basal width; style 10 μm long, reaching middle of 4th segment; peg sensillum 5 μm long. Mandible 200 μm long, and 0.30 times as long as antenna. Basal segment of maxillary palp ( Fig. 61 ) subdivided into 2 parts (p1 and p2), 110 μm in total length, and 7.3 times as long as basal width; p2 0.83 times as long as basal part p1. Ligula ( Fig. 62 ) 125 μm long, 1.9 times as long as toothed width, with basal granulose area occupying 0.24 of total length and as wide as base; middle tooth 1.8 times as long as wide. Paraligula bifid and 65 μm long; fork located 0.50 from base; outer spine 2.2 times as long as inner spine. Pecten hypopharyngis with 21 teeth.

Body. Procercus 3.1 times as long as basal width, with 7 apical setae; both lateral setae located 0.33 and 0.61, respectively, from base. Posterior parapod with 14 yellow and 2 dark brown claws, one slightly longer and thinner than the other; smallest claw sharply bent with large basal expansion, and large claws finely pectinate along inner and outer margins ( Fig. 63 ).

Remarks. Ablabesmyia (Karelia) pictipes (Kieffer) and A. (K.) nilotica (Kieffer) have been known from the Palaearctic region. The female antenna of the former has 11 segments including the pedicel (Freeman 1955, Fittkau 1962), and the tibia of the latter has four black rings (Freeman 1955). The present species can be separated in the female antenna with 12 segments, and the tibia with three rings. Furthermore the species obviously differs from all the known species of Karelia in the prominent lateral lobe in the male aedeagal complex, and the apically clubshaped aeropyle in the pupal thoracic horn.

Figures

FIGURES 52 – 55. Ablabesmyia (Karelia) makarchenkoi sp. n., male (52 – 54) and female (55). 52, Wing; 53, hypopygium, dorsal view; 54, aedeagal complex, dorsal view; 55, genitalia, ventral view.

FIGURES 56 – 63. Ablabesmyia (Karelia) makarchenkoi sp. n., pupa (56 – 59) and larva (60 – 63). 56, Abdominal segment IV, dorsal view, showing pigmentation pattern; 57, apex of thoracic horn with apical nipple; 58, abdominal segments VII – IX, dorsal view, all dorsal and left lateral setae omitted; 59, shagreen on posteromedial part of abdominal tergite IV; 60, arrangement of ventral cephalic setae and sensory pore; 61, maxillary palp with apical stylets; 62, ligula and paraligula, right paraligula omitted; 63, medium- and small-sized claws of posterior parapod.

Tables

TABLE 5. Lengths (μm) and proportions of legs of Ablabesmyia (Karelia) makarchenkoi sp. n., male (n = 1) and female (n = 5).

Male	fe	ti	ta1	ta2	ta3	ta4	ta5	LR	BR
Female	P1 889 P2 970 P3 859	1010 980 1151	707 747 939	444 394 525	333 303 384	253 222 263	152 141 152	0.70 0.76 0.82	4.0 5.7 5.5
	P1 788–929 844	949–1111 988	707–818 733	414–465 426	313–354 327	242–263 246	141–162 147	0.74–0.76
	P2 919–1081 976	939–1131 998	687–798 271	354–404 382	283–313 293	202–232 214	131 131	0.71–0.76
	P3 747–929 832	1091–1333 1178	859–1050 923	505–586 527	364–424 388	232–273 253	141–152 143	0.75–0.85