Izecksohniella puri, Sperber, Carlos Frankl, Rocha, Aline, Lopes-Andrade, Cristiano & Mesa, Alejo, 2003

Sperber, Carlos Frankl, Rocha, Aline, Lopes-Andrade, Cristiano & Mesa, Alejo, 2003, Izecksohniella puri sp. n., a new Brazilian cricket species (Ortho­ ptera: Grylloidea: Phalangopsidae) from Atlantic Forest remnants, Zootaxa 244, pp. 1-12 : 3-10

Izecksohniella puri sp. n.

Etymology. From Puri , an old indigenous tribe which inhabited Viçosa , Minas Gerais State, Brazil ( Paniago 1990), the type locality of this species.

Diagnosis. The following combination of characters is sufficient to distinguish I. puri sp. n. from I. aimore : males with both forewings coriaceous, conspicuous metanotal glands below the forewings, apices of genital projections enlarged, with a row of teeth; color pattern of female femur III and dorsum pronounced, in comparison to the paleness of I. aimore .

Description. Measurements in Table 1 . Dorsal coloration dark, contrasting to pale ventral coloration. Head homogenously reddish, distinct from the darker body color ( Fig. 1 A, B, Fig. 2 F). Three circular occelli forming a triangle, the inferior one between antennae and the other shortly above. Clypeus and labrum whitish, with short bristles. Scape longer than wide, reddish. Antennal flagellum with dark alternated with white groups of antennomeres, white groups never longer than dark ones ( Fig. 3 A). Scape and first five antennomeres dark, followed by a white, six dark, two white, four dark, two white, eight dark and four white. In the sequence, there is a color pattern variation between individuals, but always alternating longer dark and shorter white portions. First three segments of maxillary palps pale, and the two apical ones darker; first two basal segments smaller than the other, and last segment truncated at apex. Anterior lateral corners of pronotum folded in oblique angle. External surface of femur III with longitudinal color pattern as in Figure 3 B. Foretibiae without tympanum, with one pair of movable apical ventral spurs. Tibiae I and II without serrulation nor dorsal spurs. Tibia III with dorsal serrulation, four pairs of dorsal, movable, spurs, and three pairs of apical, movable spurs, median spurs longer than ventral and dorsal on each face, and external spurs smaller than internal. All tarsi with first tarsomere largest, and second smallest, with curved tarsal claws. First two tarsomeres of tarsi I and II with two raws of ventral serrulations and a pair of apical spurs, while there are no ventral serrulations on tarsus III, and its first tarsomere present two rows of dorsal and a pair of apical spurs. No glandular spurs on legs. Supra­anal plate as in Figure 3 E.

Adult males. Head coloration distinctly brighter than pronotum and body color, in dorsal view ( Fig. 1 A). Both male forewings coriaceous, covering two thirds of abdomen, without visible hairs nor stridulatory vein. Dorsal venation in high relief ( Fig. 2 A). Right wing larger and generally folded upon left wing, the latter with less defined and slightly different venation. The lateral border of the wings bent downwards, in their costal and radial area. There is a bright yellowish strip at wing’s edges, which contrast with wing’s dark brownish coloration. Males with conspicuous and distinctive meso­ and metanotal glands, covered by the forewings ( Fig. 2 B). Subgenital plate longer than wide, with posterior median invagination, forming a pouch ( Fig. 3 C), that contains genitalia and spermatophore ( Fig. 3 F).

Male genitalia. Male genitalia with lateral projections that bend dorsally to the posterior direction ( Fig. 3 G), and with a row of teeth in the dorsal face of their enlarged apices ( Fig. 2 C). Before extraction, these apices emerge laterally, one in each side, upon the subgenital plate. Sometimes each apex bears an amorphous tubular secretion extruded from its dorsal face ( Fig. 2 C). The projections present internal wrinkles, embracing a pair of complex median­posterior processes ( Fig. 2 D). These processes are large, highly sclerified and rounded structures, covered with a membranous layer in dorsum, with a rugose region in their posterior­lateral internal face. These processes present an unique ventral tooth ( Fig 2 E). In the basal region of these processes there is a group of intermingled sclerites, with a pair of clamp­like sclerites among them, dorsally visible, that may be hooked together ( Fig. 2 D, arrow). Ventrally, there is a group of median sclerites that project downwards, in the direction of the spermatophore. Some specimens may present distorted genitalia, apparently due to chemical interference in the clarification process.

Adult females. Head coloration does not differ from pronotum and body color ( Fig. 1 B). Wingless, without meso­ nor metanotal glands. Dorsal coloration of abdomen dark, with pale spots restricted to a median region. Subgenital plate with posterior median invagination, wider than long. Bursa copulatrix (= copulatory papilla) without developed basal area, with obliquely truncated apex. In adults, ovipositor with distinctly widened apex, flattened laterally ( Fig. 1 B).

Nymphs. Dorsal coloration of abdomen of both male and female nymphs dark, with pale spots restricted to a median region ( Fig. 2 F). Antennae color pattern as follows: scape and next five antennomeres dark, then one white, two dark, one white, four dark, two white, four dark, and two white. In sequence there is alternation of longer dark portions and shorter white ones. Female nymph’s ovipositor distinct from adult’s by being shorter, with pubescence along it’s entire length, and no enlarged apex.

Chromosomes. Chromosome number is 2n = 11 in males ( Fig. 4 ), 2n = 12 in females, with a XO (male), XX (female) sex determining mechanism.

Type series. Holotype adult male, labeled: " Brasil (MG), Viçosa­Frag: BIO, Data: 09­ III­2000, Sperber leg., Código: ­ # 5280A Holótipo"; allotype adult female, labeled: " Brasil (MG), Viçosa, Frag : BIO, Data: 09­III­2000, Sperber leg., Código:­ #5281. Alótipo"; adult male paratypes (50 specimens), all labeled: " Brasil (MG), Viçosa, Sperber leg. Parátipo"; distinctly labeled: "P7, 18 ­II­1996, P7B14 #390"; "BUR, 05­II­1996, M6C3 # 948"; " BUR, 14­II­1996, M3B1 # 995"; "P2, 11 ­II­1996, P2B1 # 1039"; "P12, 04 ­II­1994, P12C1 # 3058"; " BIO, 09­III­2000, # 5288"; " BIO, 09­III­2000, # 5290"; " BIO, 09­III­ 2000, #5292"; " BIO, 09­III­2000, # 5305"; " BIO, 09­III­2000, #5308"; " BIO, 02­II­2000, #5540"; " BIO, 02­II­2000, #5541"; " BIO, 02­II­2000, # 5543"; " BIO, 02­II­2000, #5546"; " BIO, 02­II­2000, #5547"; " BIO, 02­II­2000, #5548"; " BIO, 02­II­2000, # 5549"; " BIO, 02­II­2000, # 5551"; " BIO, 02­II­2000, # 5553"; " BIO, 02­II­2000, # 5573"; " BIO, 02­II­ 2000, # 5575"; " BIO, 20­I­2000, # 5576"; " BIO, 09­III­2000, # 6000"; " BIO, 09­III­2000, # 6012"; " BIO, 04­II­1997, # 6015"; " BIO, 04­II­1997, # 6016"; " BIO, 04­II­1997, # 6016"; " BIO, 04­II­1997, # 6016"; " BIO, 10­I­2002, # 6036"; " BIO, 10­I­2002, # 6037; " BIO, 10­I­2002, #6038"; " BIO, 10­I­2002, #6042"; " BIO, 10­I­2002, # 6043"; " BIO, 10­ I­2002, # 6065"; " BIO, 10­I­2002, # 6066"; " BIO, 10­I­2002, # 6069"; " BIO, 10­I­2002, #6076"; " BIO, 10­I­2002, #6076"; " BIO, 10­I­2002, #6076"; " BIO, 10­I­2002, #6076"; " BIO, 10­I­2002, #6076"; " BIO, 10­I­2002, #6076"; " BIO, 10­I­2002, #6076"; " BIO, 10­I­ 2002, #6076"; BIO, 10­I­2002, #6076"; " BIO, 10­I­2002, #6076"; " BIO, 16­III­2002, # 6134"; " BIO, 16­III­2002, # 6140"; " BIO, 16­III­2002, # 6148"; " BIO, 16­III­2002, #6150". Adult female paratypes (77 specimens): all labeled " Brasil (MG), Viçosa, Sperber leg., Parátipo, distinctly labeled: "P7, 18 ­II­1996, P7C14 # 317"; "P7, 18 ­II­1996, P7C18 #322B"; "P7, 18 ­II­1996, # 322C"; "P7, 18 ­II­1996, P7C19 # 328"; "P7, 17 ­II­1996, P7C4 # 343"; "P7, 17 ­II­1996, P7C2 # 346"; "P7, 18 ­II­1996, P7B19 # 384"; " BIO, 08­III­1998, #5280B"; " BIO, 09­III­2000, # 5289"; " BIO, 09­III­2000, # 5291"; " BIO, 09­III­2000, # 5292A; " BIO, 09­III­2000, # 5296"; " BIO, 09­III­2000, # 5306"; " BIO, 09­III­2000, # 5307"; " BIO, 08­III­1998, # 5316"; " BIO, 08­III­1998, # 5318"; " BIO, 08­III­1998, # 5319"; " BIO, 08­III­1998, # 5323"; " BIO, 08­III­1998, # 5324"; " BIO, 08­III­1998, # 5325"; " BIO, 08­III­2000, # 5326"; " BIO, 08­III­1998, # 5328"; " BIO, 08­III­1998, # 5335"; " BIO, 09­III­2000, # 5336"; " BIO, 09­III­2000, # 5337"; " BIO, 09­III­2000, # 5338"; " BIO, 09­III­2000, # 5339"; " BIO, 09­III­2000, # 6001"; " BIO, 09­III­2000, # 6012"; "Jardim Botânico­UFV, 04­II­1997, # 6013"; "Jardim Botânico­UFV, 04­II­1997, # 6013"; "Jardim Botânico­UFV, 04­II­1997, # 6013"; "Jardim Botânico­UFV, 04­II­1997, # 6013"; " BIO, 04­II­1997, # 6014"; " BIO, 10­I­2002, # 6029"; " BIO, 10­I­2002, # 6030"; " BIO, 10­I­2002, # 6041"; " BIO, 10­I­2002, # 6044"; " BIO, 10­I­2002, # 6046"; " BIO, 10­ I­2002, # 6063"; " BIO, 16­III­2002, # 6135"; " BIO, 16­III­2002, # 6136"; " BIO, 16­III­ 2002, # 6136"; " BIO, 16­III­2002, # 6136"; " BIO, 16­III­2002, # 6136"; " BIO, 16­III­ 2002, # 6136"; " BIO, 16­III­2002, # 6136"; " BIO, 16­III­2002, # 6137"; " BIO, 16­III­ 2002, # 6141"; " BIO, 16­III­2002, # 6141"; " BIO, 16­III­2002, # 6141"; " BIO, 16­III­ 2002, # 6141"; " BIO, 16­III­2002, # 6141"; " BIO, 16­III­2002, # 6142"; " BIO, 16­III­ 2002, # 6143"; " BIO, 16­III­2002, # 6143"; " BIO, 16­III­2002, # 6144"; " BIO, 16­III­ 2002, # 6144"; " BIO, 16­III­2002, # 6145"; " BIO, 16­III­2002, # 6145"; " BIO, 16­III­ 2002, # 6145"; " BIO, 16­III­2002, # 6146"; " BIO, 16­III­2002, # 6149"; " BIO, 16­III­ 2002, # 6149"; " BIO, 16­III­2002, # 6151"; " BIO, 16­III­2002, # 6151"; " BIO, 16­III­ 2002, # 6152"; " BIO, 16­III­2002, # 6153"; " BIO, 16­III­2002, # 6153"; " BIO, 16­III­ 2002, # 6153"; " BIO, 16­III­2002, # 6153"; " BIO, 16­III­2002, # 6153"; " BIO, 19­VI­ 2000, # 6159"; " BIO, 09­III­2000, # 6169"; " BIO, 11­V­2000, # 6171"; " BIO, 11­V­2000, # 6172"; " BIO, 11­V­2000, # 6173"; " BIO, 11­V­2000, # 6179"; male nymphs (24 specimens), distinctly labeled: " BIO, 10­I­2002, # 6028"; " BIO, 10­I­2002, # 6073"; " BIO, 10­ I­2002, # 6073"; " BIO, 10­I­2002, # 6073"; " BIO, 10­I­2002, # 6073"; " BIO, 10­I­2002, # 6073"; " BIO, 10­I­2002, # 6075"; " BIO, 10­I­2002, # 6097"; " BIO, 10­I­2002, # 6098"; " BIO, 10­I­2002, # 6111"; " BIO, 10­I­2002, # 6111"; " BIO, 10­I­2002, # 6113"; " BIO, 10­ I­2002, # 6113"; " BIO, 10­I­2002, # 6114"; " BIO, 10­I­2002, # 6114"; " BIO, 10­I­2002, # 6114"; " BIO, 10­I­2002, # 6117"; " BIO, 10­I­2002, # 6117"; " BIO, 10­I­2002, # 6122"; " BIO, 10­I­2002, # 6122"; " BIO, 10­I­2002, # 6122"; " BIO, 10­I­2002, # 6122"; " BIO, 10­ I­2002, # 6122"; " BIO, 10­I­2002, # 6122"; female nymphs (24 specimens), distincly labeled: " BIO, 10­I­2002, # 6039"; " BIO, 10­I­2002, # 6040"; " BIO, 10­I­2002, # 6057"; " BIO, 10­I­2002, # 6074"; " BIO, 10­I­2002, # 6074"; " BIO, 10­I­2002, # 6074"; " BIO, 10­ I­2002, # 6074"; " BIO, 10­I­2002, # 6074"; " BIO, 10­I­2002, # 6074"; " BIO, 10­I­2002, # 6074"; " BIO, 10­I­2002, # 6093"; " BIO, 10­I­2002, # 6111"; " BIO, 10­I­2002, #6115"; " BIO, 10­I­2002, #6115"; " BIO, 10­I­2002, #6115"; " BIO, 10­I­2002, #6115"; " BIO, 10­I­ 2002, # 6120"; " BIO, 10­I­2002, # 6120"; " BIO, 10­I­2002, # 6120"; " BIO, 10­I­2002, # 6120"; " BIO, 10­I­2002, # 6120"; " BIO, 16­III­2002, # 6135"; " BIO, 16­III­2002, # 6135"; " BIO, 02­II­2000, # 5575.

Specimens preserved in alcohol 75%. “P7”, “BUR”, “P2”, “ BIO ” are codes for different forest remnants; “M6C3”, “M3B1”, “P2B1”, “P12C1”, “P7C14”, “P7C18”, “P7C19”, “P7C4”, “P7C2” are codes for site within the remnant and pitfall trap within the site. All codes as in Sperber (1999).

Depositories. Holotype male, allotype female, and a pair of paratypes at the Museu de Zoologia da Universidade de São Paulo, São Paulo, Brazil. One pair of paratypes in each of the following collections: Academy of Natural Sciences of Philadelphia, New York, USA; Departamento de Biologia da UNESP, Campus de Rio Claro, Rio Claro, São Paulo, Brazil; Departamento de Zoologia, IB, UNESP, Botucatu, São Paulo, Brazil; Museum National d’Histoire Naturelle, Paris, France. The remaining paratypes in the Unidade de Estudos em Ecologia ( UEEC), affiliated to the Museu de Entomologia da Universidade Federal de Viçosa, Viçosa , MG, Brazil.

Figures

FIGURE 1. Izecksohniella puri sp. n. A. male; B. female. Scale bars: 5 mm.

FIGURE 2. Izecksohniella puri sp. n. A. male forewings; B. male metanotal gland; C. apex of lateral projections; D. posterior view of male genitalia; E. details of median posterior process, ventral view; F. female nymph. Scale bars: 2 mm (A, B), 5 mm (F).

FIGURE 3. Izecksohniella puri sp. n. A. adult antenna; B. femur III, external view; C. male subgenital plate; D. female subgenital plate; E. supra­anal plate, F. spermatheca; G. male genitalia, dorsal view. Scale bar: 2 mm (A, G), 10 mm (B).

FIGURE 4. Izecksohniella puri sp. n. male chromosomes, diplotene. Scale bar: 10 m.

Tables

TABLE 1. Adult Izecksohniella puri sp. n. measurements. Sex, mean, standard deviation (sd), maximum (max) and minimum (min) values, and number of measured individuals (n). (*) variable, due to body stretching.

	Sex	mean	sd	max	min	n
Femur III	F	10.7	0.4	11.3	9.8	26
Tibia III	F	11	0.5	12.3	9.9	26
Body Length* Ovipositor	F F	11.6 9.9	1.5 0.8	14.4 11.3	8.9 6.5	28 28
MedPL	F	2	0.3	3.1	1.4	28
MaxPW	F	3.3	0.6	3.8	0.3	28
MaxEW	F	2.7	0.1	2.9	2.5	28
Femur III	M	9.6	0.7	10.8	8	24
Tibia III	M	10.2	0.7	11.6	8.9	24
Body Length* MedPL	M M	10.3 1.7	1.9 0.2	13.4 2.1	7.1 1.4	18 24
MaxPW	M	2.8	0.2	3.2	2.4	24
MaxEW	M	2.4	0.1	2.6	2.1	24