Trichoderma perviride W.T. Qin & W.Y. Zhuang, 2017

Qin, Wen-Tao & Zhuang, Wen-Ying, 2017, Seven new species of Trichoderma (Hypocreales) in the Harzianum and Strictipile clades, Phytotaxa 305 (3), pp. 121-139 : 130

Trichoderma perviride W.T. Qin & W.Y. Zhuang , sp. nov. FIGURE 5

Etymology:— The specific epithet refers to the dark green colony.

MycoBank: MB 816632

Type: — CHINA. HUBEI: Shennongjia, Qiaonenggou, 1950 m, on rotten twigs, 16 Sep 2014, W. T. Qin, Z.Q. Zeng, H.D. Zheng & K. Chen 9757 (holotype HMAS 273786).

Colony radius on CMD 50–55 mm after 72 h, mycelium covering the plate after 4 d at 25 C. Colony dense, circular with well-defined margin, aerial hyphae abundant, conidiation first noted on and around the inoculum, granules or pustules whitish, gradually green. Conidiophores trichoderma-like with phialides solitary or in whorls of 2–3(–4) arising from the main axis. Phialides lageniform or ampulliform, (4–)5–8 × 1.7–2.3(–2.7) μm, l/w (2–)2.3–3.6(–3.9), 1.1–1.7 μm wide at the base (n = 50). Conidia green, globose or subglobose, smooth, 2.8–3.5 × 2.7–3.3 μm, l/ w 1.0 –1.1 (n = 50). No distinct odor; no diffusing pigment observed.

Colony radius on PDA 45–48 mm after 72 h, mycelium covering the plate after 5 d at 25 C. Colony whitish, conspicuously dense with distinct circular outline. Aerial hyphae numerous, densely disposed and radial towards the margin. Conidiation starting on and around the inoculum in short minute shrubs, spreading, tufts or pustules with surface downy developing, farinose to floccose, later indistinctly and irregularly zonate, green. Chlamydospores terminal or intercalary, globose or ellipsoidal, (4.5–)5–6(–7) × (4.5–)5–5.5(–6.5) μm, l/ w 1.0 –1.5 (n = 40). No distinct odor; no diffusing pigment observed.

Colony radius on SNA 14–18mm after 72 h, mycelium covering the plate after 10 d at 25 C. Colony semitransparent, circle, typically not zonate. Aerial hyphae scant; tufts or pustules green, sparsely arranged throughout the plate, green. No distinct odor; no diffusing pigment observed.

Notes:— In the Harzianum clade, T. perviride is closely related to T. corneum and T. christiani ( Figure 1 ). Trichoderma corneum differs in having verticillium-like conidiophores, larger phialides [8–24 × 1.5–3.0 μm], and narrower conidia [2.0–5.0 × 1.6–2.8 μm] ( Doi 1972, Chaverri & Samuels 2003). Although T. christiani has similar conidia, it forms yellow-green conidiation zones on PDA, grows faster on SNA [43–47 mm], and produces larger phialides [(5–)6–11(–16) × (2.5–)2.7–3.5(–3.8) μm] ( Jaklitsch & Voglmayr 2015).

Figures

FIGURE 1. Maximum parsimony phylogram reconstructed from the combined sequences of RPB2 and TEF1-α. MPBP (left) above 50% and BIPP (right) above 90% are indicated at the nodes. New species proposed are indicated in boldface. TreeBASE no. S19229.

FIGURE 5. Trichoderma perviride (HMAS 273786). a–c. Cultures after 13 d at 25 C (a. CMD, b. PDA, c. SNA). d–i. Conidiophores (CMD, 4 d). j. Conidia (CMD, 4 d). Scale bars: a–c = 20 mm; d–i = 20 μm; j = 10 μm.