Phyllodromica iberica, Published, 2007

KNEBELSBERGER, THOMAS & MILLER, MICHAEL A., 2007, Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding), Zootaxa 1522 (1), pp. 1-68 : 14-15

Phyllodromica iberica morph #1

( Figs 1 A–C , 3 A–I , 4 A–I , 5 A–I , 8 H , 17 )

Phyllodromica subaptera morph #1.—Knebelsberger & Bohn, 2003.

Material. Numerous specimens from the following localities: Spain. Sp 12b, 13a, b, 84b, 85, 95a, 96a, b, 97, a, b, c, 98, a, 99a, b, 100, a, b, 119, b, 134, 135, 137, a, 189, 270, a, b, c, 272, 273, 274, 276, 292, a,b, 294, 295, 296, 300, 302, 307, 324, 330, 331, 332, 334, 335, 336, 337, 366, 366a, 367, 368, 371, 375, 376, 378a, 379, 380a, 382, a, 386, a, 388, a, 389, a, 390, 391, 394, 418, 419, 420, 421, 422, 423, 426, 427, 428, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 446, a, 447, a, 448a, 449, 450, 453, 455, 463, 464, a, b, 467, a, 471a, 492, a, b, 506, 507, a, 509, 510, 511, 512, a. Material from museum collections: I: 1 ♂ [ Spain, Prov. Cuenca] Uclés, Père Pantel dedit, coll. A. Finot ( MNHN). II: 1 ♂ [ Spain] Nuevo Baztán , [Prov.] Madrid, 10. V .1928 ( MNMS) .

Description. Size. Length of pronotum: ♂ 1.34–1.6 (mean 1.5) mm; ♀ 1.81–1.95 (mean 1.89) mm.

Legs. Distal end of mid tibia with 5 spines ( Fig. 5 E ).

Male

Tergites. —Tergite 6. Emargination of the posterior border relatively broad, without a transversal torus behind the median ridge ( Figs 3 C , 5 A ). Tergites 7 and 8. Cuticule and glandular structures of Phyllodromica iberica morph #1 strongly sculptured as already described in detail in the section “The characteristics of the subaptera -group”. Median lobe of T7 short, shorter than distance between bristle fields and the posterior bor- der of the tergite, almost without glandular pores ( Fig. 3 D ). Tergite 10, Supraanal plate triangularely rounded ( Fig. 3 G ).

Sternites. —Paraproct. Medio-anterior process of the right paraproct without a bulge ( Figs 3 G , 5 D ).

Genitalia. —Helmet sclerite. “Frontal” part and in the “rear” reduced (see section “The characteristics of the subaptera -group”).

Colouration.— Tergites 2–5. Posterior extensions of the dark anterior area usually weakly developed or missing ( Fig. 3 B ). Tergite 6. Dark area may reach near to the posterior border of the tergite leaving at least a narrow light margin, often lightened to various extents along the longitudinal midline and along a transversal line shortly behind the ridge ( Fig. 3 C ), sometimes reduced to two relatively small clusters of dark spots behind the ridge ( Fig. 5 A ). Tergite 7. Posterior and lateral slopes of the mound very dark (brown), remaining parts of the dark area much lighter coloured, at the bottom of the trough in the middle with a whitish patch ( Fig. 3 D ). Tergite 8. Median dark extension broad, laterally often bordered by anterior projections of the light posterior area sometimes reaching the transversal edge ( Fig. 5 B ), or at the same position near the edge with a pair of light patches ( Fig. 3 E ). Mediolateral extensions usually indistinct. Conical process as a rule lightly coloured ( Fig. 5 B ).

Female ( Fig. 5 F–I ).

For description of legs, genitalia and colouration see description of P. subaptera .

Genetic Data. 1597 basepairs of the mitochondrial genome including the complete cytochrome c oxidase subunit I (COI) gene were analysed in two paratype specimens (see above). Phyllodromica iberica _ Paratype Sp 292b/M1_1597bp and Phyllodromica iberica _ Paratype Sp 292b/M2_1597bp share the same sequence. The complete sequences are shown in Appendix 4.

Geographical distribution ( Fig. 16 ). Widely distributed in Spain, except in the northeastern and northwestern corners and the south, presumably also occurring in Portugal (females at localities Po 19 and 24, Figs 15, 17 ).

Other species of the subaptera -group found. P. subaptera: Sp 419.

Other morphs of P. iberica found. Morph #2: Sp 366, 512; morph #3: Sp 270, 335, 367, 375, 380, 386, 388, 389, 446, 448, 449, 507, 512.

Figures

FIGURE 1. A–C P. iberica morph #1, habitus. (A) Male in dorsal view; (B) female in dorsal and (C) ventral view. D Oothek of P. quadracantha (lateral view). Abbreviations: a antenna, c cercus, fl coxa of foreleg, h head, hl coxa of hindleg, mes mesonotum, met metanotum, ml coxa of midleg, pm palpus maxillaris, pro pronotum, sub subgenital plate, T2–T10 tergites 2–10, tm tegmen. Same scale in A and B. Localities: (A–C) Sp 449a, (D) Sp 500.

FIGURE 3. Phyllodromica iberica morph #1, male (holotype). A Thoracal nota. B–F Abdominal tergites 5–9. (B) Tergite 5 and (C) tergite 6 with membranous glands in the lateral region of the anterior margin of tergites (black arrows); (D) tergite 7, white arrow heads point to the shallow pouches appearing as crescent–shaped black shadows; (E) tergite 8; (F) tergite 9 on glass rod. G Terminalia with tergite 10 (sa), cerci and paraprocts. H Hook of left phallomere with the posterior end on the top. I Subgenital plate with remaining genital sclerites (without hook). Abbreviations: ap anterior process, bf bristle field, c cercus, cm central mound, cp conelike process, ll lateral lobe, m mound, mes mesonotum, met metanotum, ml median lobe, mp medio-anterior process, pml posterior median lobe, pro pronotum, r ridge between bristle fields, rp right paraproct, sa supraanal plate, se sinusoidal edge, sp shallow pits, tm tegmen, tr transversal ridge, tt transversal trough. Same scale for (B, C, G) and (D, E, H). Identification: Sp 292b/M3 (holotype).

FIGURE 4. Phyllodromica iberica morph #1, male, SEM pictures of tergal glandular structures. A–C, G Tergite 7. (A) Latero-frontal view from slightly above, anterior wall of trough slightly hollowed out anteriorly (white arrows); (B) lateral view of the glandular region from slightly above; (C) glandular region in dorsal view; (G) bristles of bristle field. D– F, H, I Tergite 8. (D) Dorsal view of the whole tergite, median emargination in the distribution of the bristles (white arrow heads); (E) latero-frontal view from slightly above; (F) dorsal view of the conelike process; (H) porous surface of the central mound; (I) soft villi on the surface of the conelike process. Abbreviations: bf bristle field, cm central mound, cp conelike process, m mound, r ridge, se sinusoidal edge, sp shallow pit, tt transversal trough. Identification: (A–C, E– I) Sp 270a/M4, (D) Sp 85/11.

FIGURE 5. Phyllodromica iberica morph #1, A–E, male. (A) Tergite 7 and (B) tergite 8 of a male specimen differing from the holotype in its colouration; (C) membrane glands of the left lateral region on the anterior border of tergite 6; (D) right paraproct; (E) distal end of tibia of the right mid leg (bearing 5 distal spines) in posterior view. F–I, female. (F, H) Different colour pattern of pronotum; (G, I) tergite 5. Abbreviations: mg membrane glands, mp medio-anterior process of right paraproct, rp right paraproct, sp spinelike process of right paraproct, tar tarsus, tib tibia. Same scale for (C, D) and (F–I). Identification: (A, B) Sp 330/3, (C, D) Sp 292b/M3 (holotype), (E) Sp 510/M1, (F, G) Sp 292b/W1, (H, I) Sp 292b/W2.

FIGURE 8. A–E Phyllodromica iberica morph #2, male. (A–D) Different colour patterns of tergite 6; (E) helmet sclerite. F, G Phyllodromica iberica morph #2, female. (F) Thoracal nota; (G) abdominal tergite 5. H Right front leg of Phyllodromica iberica morph #1, male in frontal view. I Right front leg of Phyllodromica quadracantha, male in frontal view. Abbreviations: fr “frontal” part of helmet sclerite, cox coxa, fem femur, hs helmet sclerite re “rear” of helmet sclerite, tib tibia, tm tegmen. Same scale for (A–D), (F, G) and (H, I). Identification: (A) Sp 266/2, (B) Sp 365/3, (C) Sp 365a/M14, (D) Sp 365a/M8, (E) Sp 267c/M1, (F, G) Sp 267c/W2, (H) Sp 510/M11, (I) Sp 203d/M1 (holotype)..

FIGURE 16. Distribution of bisexual species of the subaptera-group and the parthenogenetic species P. subaptera on the Iberian peninsula.

FIGURE 17. Distribution of the bisexual species of the subaptera-group: Phyllodromica iberica morph #1, #2 and #3, and P. quadracantha; these species only occur on the Iberian Peninsula. In cases where two different symbols partly overlap, two P. iberica morphs are found at the same locality; in one case all three morphs occur together (Fig. 15, Sp 510 ca. 1° W, 40° N). P. iberica morph #3 symbols labeled with a “+” (Fig. 15, Sp 335) or an “ ” (Fig. 15, Sp 186, 270, 469 and 512) indicate the presence of morphological variations. At some localities no males have been found (“sad face” symbol) but females with spermathecae containing sperms and/or with oothecae containing male and female offspring. Both facts indicate the presence of a bisexual species of unknown specifity.