Methylobacterium gregans

Maeng, Soohyun, Kim, Ju-Young, Jang, Jun Hwee & Kim, Myung-Suk Kang and Myung Kyum, 2018, A report of 11 unrecorded bacterial species in Korea isolated in 2017, Journal of Species Research 7 (2), pp. 135-150 : 139-140

Description of Methylobacterium gregans 17Sr1_23

Cells are Gram-stain-negative, non-flagellated, and short rod-shaped. Colonies are pink-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, D-glucuronic acid and formic acid is utilized as a sole carbon source. But acetoacetic acid, N -acetyl-D-mannosamine, L-alanine, L-arginine, L-aspartic acid, D-cellobiose, dextrin, D-fructose, D-fructose 6-PO 4, L-galactonic acid lactone, D-galactose, D-galacturonic acid, gelatin, gentiobiose, D-gluconic acid, α -D-glucose, glucuronamide, L-glutamic acid, inosine, α -keto-glutaric acid, L-malic acid, D-maltose, D-mannitol, D-mannose, D-melibiose, β -methyl-D-glucoside, 3-methyl glucose, myo-inositol, pectin, propionic acid, glycyl-L-proline, L-pyroglutamic acid, quinic acid, D-raffinose, L-rhamnose, L-serine, stachyose, sucrose, D-trehalose, D-turanose acetic acid, N -acetyl-D-galactosamine, N -acetyl-neuraminic acid, N -acetyl-D-glucosamine, γ -amino-butryric acid, D-arabitol, D-aspartic acid, bromo-succinic acid, citric acid, D-fucose, L-fucose, D-gluco se-6- PO 4, glycerol, L-histidine, α -hydroxybutyric acid, β -hydroxy-D,L-butyric acid, p -hydroxy-phenylacetic acid, α -keto-butyric acid, L-lactic acid, D-lactic acid methyl ester, α -D-lactose, D-malic acid, methyl pyruvate, mucic acid, D-saccharic acid, D-salicin, D-serine, D-sorbitol, and tween 40 were not utilized. In sensitivity tests, the tetrazolium redox dye is reduced in the presence of lincomycin, vancomycin, tetrazolium blue, nalidixic acid, potassium tellurite, and aztreonam; but not in the presence of 1% NaCl, 1% sodium lactate, 4% NaCl, 8% NaCl, guanidine HCl, lithium chloride, pH 6, D-serine, sodium butyrate, tetrazolium violet fusidic acid, minocycline, niaproof 4, pH 5, rifamycin SV, sodium bromate, and troleandomycin.

In API 20NE system, positive for the reduction of nitrates (NO 3) to nitrogen (N 2), arginine dihydrolase, urease, esculin hydrolysis, β -galactosidase, D-mannose, and D-maltose. Negative for the reduction of nitrates (NO 3) to nitrite (NO 2 -), indole production on tryptophan, glucose fermentation, gelatin hydrolysis, D-glucose, L-arabinose, D-mannitol, N -acetyl-D-glucosamine, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate, and phenylacetic acid.

In API 32GN system, positive for itaconic acid, suberic acid, lactic acid, L-alanine, propionic acid, trisodium citrate, and 3-hydroxybutyric acid. Negative for L-rhamnose, N -acetyl-glucosamine, D-ribose, inositol, D-saccharose (sucrose), D-maltose, sodium malonate, sodium acetate, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L-serine, D-mannitol, D-glucose, salicin, D-melibiose, D-fucose, D-sorbitol, L-arabinose, capric acid, valeric acid, L-histidine, potassium 2-ketogluconate, 4-hydroxybenzoic acid, and L-proline. Strain 17Sr1_23 (= NIBRBAC000500515) was isolated from a soil sample.