Methylobacterium aerolatum

Maeng, Soohyun, Kim, Ju-Young, Jang, Jun Hwee & Kim, Myung-Suk Kang and Myung Kyum, 2018, A report of 11 unrecorded bacterial species in Korea isolated in 2017, Journal of Species Research 7 (2), pp. 135-150 : 144-145

Description of Methylobacterium aerolatum 16B15D

Cells are Gram-stain-negative, non-flagellated, and short rod-shaped. Colonies are pink-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, acetoacetic acid, N -acetyl-D-mannosamine, L-alanine, L-arginine, L-aspartic acid, D-cellobiose, dextrin, D-fructose, D-fructose 6-PO 4, L-galactonic acid lactone, D-galactose, D-galacturonic acid, gelatin, gentiobiose, D-gluconic acid, α -D-glucose, glucuronamide, D-glucuronic acid, L-glutamic acid, inosine, α -keto-glutaric acid, L-malic acid, D-maltose, D-mannitol, D-mannose, D-melibiose, β -methyl-D-glucoside, 3-methyl glucose, myo-inositol, pectin, propionic acid, glycyl-L-proline, L-pyroglutamic acid, quinic acid, D-raffinose, L-rhamnose, L-serine, stachyose, sucrose, D-trehalose, D-turanose acetic acid, N -acetyl-D-galactosamine, N -acetyl-neuraminic acid, N -acetyl-D-glucosamine, γ -amino-butryric acid, D-arabitol, D-aspartic acid, bromo-succinic acid, citric acid, formic acid, D-fucose, L-fucose, D-glucose-6-PO 4, glycerol, L-histidine, α -hydroxybutyric acid, β -hydroxy-D, L-butyric acid, p -hydroxy-phenylacetic acid, α -keto-butyric acid, L-lactic acid, D-lactic acid methyl ester, α -D-lactose, D-malic acid, methyl pyruvate, mucic acid, D-saccharic acid, D-salicin, D-serine, D-sorbitol, and tween 40 were not utilized. In sensitivity tests, the tetrazolium redox dye is reduced in the presence tetrazolium violet; but not in the presence of 1% NaCl, 1% sodium lactate, 4% NaCl, 8% NaCl, guanidine HCl, lithium chloride, pH 6, potassium tellurite, D-serine, sodium butyrate, aztreonam, fusidic acid, lincomycin, minocycline, nalidixic acid, niaproof 4, pH 5, rifamycin SV, sodium bromate, tetrazolium blue, troleandomycin, and vancomycin.

In API 20NE system, positive for the reduction of nitrates (NO 3) to nitrite (NO 2 -), arginine dihydrolase, urease, D-glucose, L-arabinose, D-mannose, D-mannitol, D-maltose, potassium gluconate, adipic acid, and malic acid. Weak positive for trisodium citrate. Negative for the reduction of nitrates (NO 3) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, esculin hydrolysis, gelatin hydrolysis, β -galactosidase, N -acetyl-D-glucosamine, capric acid, and phenylacetic acid.

In API 32GN system, positive for D-ribose, itaconic acid, D-glucose, and L-arabinose. Negative for L-rhamnose, inositol, N -acetyl-glucosamine, D-saccharose (sucrose), D-maltose, suberic acid, sodium malonate, sodium acetate, lactic acid, L-alanine, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L-serine, D-mannitol, salicin, D-melibiose, D-fucose, D-sorbitol, propionic acid, capric acid, valeric acid, trisodium citrate, L-histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid, L-serine, 4-hydroxybenzoic acid, and Lproline. Strain 16B15D (= NIBRBAC000500519) was isolated from a soil sample.