Ruinenia lunata Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 110

Ruinenia lunata Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828806. Fig. 14K, L .

Etymology: the specific epithet lunata refers to the falcate ballistoconidia of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal to falcate, 1.8–3.5 × 5.0 –9.0 μm and single, budding is polar ( Fig. 14K ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is orange-red, butyrous, smooth. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are reniform to falcate, 3.0 –6.5 × 6.0–13.0 μm ( Fig. 14L ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose (variable), trehalose, melibiose, raffinose, melezitose, ribitol (delayed), Dmannitol (delayed) and D-glucitol (delayedand weak) are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, inulin, soluble starch, L-rhamnose, D-xylose, L-arabinose, D-arabinose, D-ribose, D-glucosamine, methyl α- Dglucoside, methanol, ethanol, erythritol, galactitol, glycerol, DLlactic acid, critic acid, salicin, succinic acid, inositol and hexdecane are not assimilated. Ammonium sulfate and ethylamine hydrochloride (variable) are assimilated as sole nitrogen sources. L-lysine, sodium nitrite, potassium nitrate and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 22 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ru. lunata differs from its closely related species Ru. bangxiensis and Ru. clavata in its inability to assimilate soluble starch and D-xylose and grow at 25 °C ( Table S1.20 ).

Typus: China, Taiwan province, obtained from a leaf of an unidentified plant, Aug. 2009, Q.-M. Wang (holotype CGMCC 2.4426 T preserved in a metabolically inactive state, ex-type CBS 12525 = TW 2.1E-028).

Figures

Fig. 14. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Kon. rhododendri CGMCC 2.3463T; (C, D) Ben. wuzhishanensis CGMCC 2.3569T; (E) Ben. pseudorectispora CGMCC 2.5677T; (F) Ps. fusiformis CGMCC 2.5823T; (G, H) Ru. fanjingshanensis CGMCC 2.4542T; (I, J) Ru. bangxiensis CGMCC 2.3454T; (K, L) Ru. lunata CGMCC 2.4426T; (M, N) Boe. sterigmata CGMCC 2.4539T, (O) St. layueensis CGMCC 2.5817T; (P) Pse. motuoensis CGMCC 2.5816T. Bars = 10 μm.