Phaeotremella ovata Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 98-99

Phaeotremella ovata Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828783. Fig. 10J .

Etymology: the specific epithet ovata refers to the ovoid vegetative cells of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and fusiform, 2.0– 3.4 × 4.8–8.2 μm and single, budding is polar ( Fig. 10J ), a sediment is formed. After 1 mo at 17 °C, a pellicle and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellow, butyrous, smooth. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, melezitose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, ribitol, galactitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside, salicin and D-gluconate are assimilated as sole carbon sources. L-sorbose, raffinose, methanol, ethanol, glycerol, erythritol, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Pha. ovata differs from its closely related species Pha. lactea in its inability to assimilate raffinose, succinate and myo-inositol and its ability to assimilate soluble starch, N-Acetyl-D-glucosamine, Methyl-α- D-glucoside and cadaverine ( Table S1.12 ).

Typus: China, Nanwenghe , Heilongjiang province, obtained from a leaf of an unidentified plant, Aug. 2015, Q.-M. Wang (holotype CGMCC 2.5614 T preserved in a metabolically inactive state, ex-type CBS 15756 = NW9D3).

Figures

Fig. 10. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) De. melastomatis CGMCC 2.3459T; (C, D) De. taiwanicus CGMCC 2.4429T; (E, F) De. ovatus CGMCC 2.3572T; (G, H) De. longicylindricus CGMCC 2.5660T;; (I) Pha. lactea CGMCC 2.5810T; (J) Pha. ovata CGMCC 2.5614T; (K, L) Ho. saccardoi CGMCC 2.3445T; (M) So. gelidoterrea CGMCC 2.5814T; (N) Fi. dingjieense CGMCC 2.5649T, (O) Fi. globosum CGMCC 2.5680T; (P) Fi. mali CGMCC 2.4012T. Bars = 10 μm.