Filobasidium dingjieense Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 100

Filobasidium dingjieense Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828786. Fig. 10N .

Etymology: the specific epithet dingjieenese refers to the geographic origin of the type strain, Dingjie county, Tibet.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are globosal and ellipsoidal, 6.8–10.6 × 6.9–10.6 μm and single, budding is polar ( Fig. 10N ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present.On YM agar, after 1 mo at 17 °C, the streak culture is gray-cream, mucoid, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed and weak), sucrose, maltose, cellobiose, trehalose, melezitose, D-xylose, Larabinose, ethanol (delayed and weak), glycerol (delayed and weak), Methyl-α- D-glucoside (weak), succinate, citrate and myo-inositol are assimilated as sole carbon sources. L-sorbose, lactose, melibiose, raffinose, inulin, soluble starch, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, salicin, DL-lactate and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, ethylamine hydrochloride (delayed and weak) and cadaverine dihydrochloride (delayed and weak) are assimilated as sole nitrogen sources. L-lysine is not assimilated. Maximum growth temperature is 19 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Fi. dingjieense differs from its closely related species Fi. uniguttulatum in its inability to assimilate raffinose, Lrhamnose, N-Acetyl-D-glucosamine, ribitol, D-mannitol, D-glucitol, salicin, hexadecane and L-lysine and its ability to assimilate cellobiose, potassium nitrate and sodium nitrite ( Table S1.15 ).

Typus: China, Dingjie county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5649 T preserved in a metabolically inactive state, ex-type CBS 15567 = GPS3.2A5).

Figures

Fig. 10. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) De. melastomatis CGMCC 2.3459T; (C, D) De. taiwanicus CGMCC 2.4429T; (E, F) De. ovatus CGMCC 2.3572T; (G, H) De. longicylindricus CGMCC 2.5660T;; (I) Pha. lactea CGMCC 2.5810T; (J) Pha. ovata CGMCC 2.5614T; (K, L) Ho. saccardoi CGMCC 2.3445T; (M) So. gelidoterrea CGMCC 2.5814T; (N) Fi. dingjieense CGMCC 2.5649T, (O) Fi. globosum CGMCC 2.5680T; (P) Fi. mali CGMCC 2.4012T. Bars = 10 μm.