Solicoccozyma gelidoterrea Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 99-100

Solicoccozyma gelidoterrea Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828785. Fig. 10M .

Etymology: the specific epithet gelidoterrea refers to the cold environments origin of all strains used in this study.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and ovoid, 3.3–4.8 × 4.1– 5.5 μm and single, budding is polar ( Fig. 10M ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose, sucrose, maltose, cellobiose, trehalose (variable), lactose, melibiose (variable), raffinose, melezitose, inulin, D-xylose, L-arabinose, Darabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, ethanol, ribitol, galactitol, glycerol (variable), Dmannitol, D-glucitol, Methyl-α- D-glucoside, salicin, D-gluconate and myo-inositol are assimilated as sole carbon sources. Soluble starch, methanol, erythritol, DL-lactate, succinate, citrate and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Maximum growth temperature is 32 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, So. gelidoterrea differs from its four closely related species, So. aeria , So. terrea , So. phenolica and So. fuscescens , in its inability to assimilate succinate and its ability to assimilate inulin ( Table S1.14 ).

Typus: China, Daxinganling , obtained from soil, Aug. 2015, Q.- M. Wang (holotype CGMCC 2.5814 T preserved in a metabolically inactive state, ex-type CBS 15580 = HFB003-3).

Figures

Fig. 10. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) De. melastomatis CGMCC 2.3459T; (C, D) De. taiwanicus CGMCC 2.4429T; (E, F) De. ovatus CGMCC 2.3572T; (G, H) De. longicylindricus CGMCC 2.5660T;; (I) Pha. lactea CGMCC 2.5810T; (J) Pha. ovata CGMCC 2.5614T; (K, L) Ho. saccardoi CGMCC 2.3445T; (M) So. gelidoterrea CGMCC 2.5814T; (N) Fi. dingjieense CGMCC 2.5649T, (O) Fi. globosum CGMCC 2.5680T; (P) Fi. mali CGMCC 2.4012T. Bars = 10 μm.