Kondoa foliicola Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 104

Kondoa foliicola Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828794. Fig. 12G, H .

Etymology: the specific epithet foliicola refers to the substrate origin of the type strain, leaves.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and somewhat ovoid, 3.1– 5.4 × 5.1–7.8 μm and single, budding is polar ( Fig. 12G ), a sediment is formed. After 1 mo at 17 °C, an incomplete ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pale-yellow, butyrous, dull. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal or ovoid, 2.5– 4.0 × 3.8– 8.8 μm ( Fig. 12H ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, cellobiose, trehalose, lactose, raffinose, melezitose, soluble starch, D-xylose, L-arabinose, Darabinose, glucosamine, glycerol, ribitol and D-mannitol are assimilated as sole carbon sources. Galactose, L-sorbose, maltose, melibiose, inulin, D-ribose, L-rhamnose, D-N-methanol, ethanol, erythritol, galactitol, D-glucitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), L-lysine and ethylamine hydrochloride (delayed and weak) are assimilated as sole nitrogen sources. Sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26–27 °C. Growth in vitamin-free medium is negative. Starch-like substances are produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Kon. foliicola differs from its closely related species Kon. arboricola in its inability to assimilate maltose, grow in vitamin-free medium and produce starch like compounds and its ability to assimilate melezitose, D-arabinose and D-glucosamine ( Table S1.17 ).

Typus: Germany, obtained from a leaf of an unidentified plant, Sep. 2005 (holotype CGMCC 2.3100 T preserved in a metabolically inactive state, ex-type CBS 15465 = G9.1).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.