Phaffia aurantiaca Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 103

Phaffia aurantiaca Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828791. Fig. 12B .

Etymology: the specific epithet aurantiaca refers to the orange colony colour of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 3.4–6.4 × 5.2– 8.9 μm and single, budding is polar ( Fig. 12B ), a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is orange, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, Dxylose (delayed and weak), L-arabinose, D-ribose, ethanol, glycerol, erythritol, ribitol (delayed and weak), galactitol (delayed and weak), D-mannitol, D-glucitol, Methyl-α- D-glucoside (delayed and weak), salicin (delayed and weak), DL-lactate and succinate are assimilated as sole carbon sources. L-sorbose, inulin, soluble starch, D-arabinose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. Maximum growth temperature is 23 °C. Growth in vitamin-free medium is negative. Starch-like substances are produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Pha. aurantiaca differs from its closely related species Pha. rhodozyma in its inability to assimilate soluble starch and its ability to assimilate galactose, lactose, melibiose, erythritol and ethylamine ( Table S1.16 ).

Typus: China, Lulang county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5601 T preserved in a metabolically inactive state, ex-type CBS 15548 = GPS23.2A4).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.