Dioszegia kandeliae Q.M. Wang, F.Y. Bai, L.D. Guo & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 90

Dioszegia kandeliae Q.M. Wang, F.Y. Bai, L.D. Guo & A.H. Li sp. nov. MycoBank MB828763. Fig. 8I .

Etymology: the specific epithet kandeliae refers to Kandelia , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal to subglobosal, 2.5– 4.2 × 3.2– 5.5 μm and single, budding is polar ( Fig. 8I ), a ring and a sediment are formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is orange-red, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, melezitose, inulin (weak), soluble starch (delayed and weak), D-xylose (delayed and weak), L-arabinose (delayed and weak), Dglucosamine (delayed and weak), N-Acetyl-D-glucosamine (delayed and weak), ethanol (delayed and weak), glycerol (delayed and weak), ribitol (delayed and weak) and D-glucitol are assimilated as sole carbon sources. Raffinose, D-arabinose, Dribose, L-rhamnose, methanol, erythritol, galactitol, D-mannitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Sodium nitrite and L-lysine are not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is negative. Starch-like substances are produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Di. kandeliae and the closely related species Di. ovata , Di. maotaiensis , Di. zsoltii , Di. catarinoi , Di. takashimae and Di. athyrii can be distinguished from one another. Di. kandeliae differs from the other six species in its inability to assimilate raffinose and L-rhamnose ( Table S1.9 ).

Typus: China, Beilunhekou natural reserve , Guangxi province, obtained from a leaf of Kandelia candel, Apr. 2014 , L.-D. Guo (holotype CGMCC 2.5658 T preserved in a metabolically inactive state, ex-type CBS 13951 = 224191).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.