Kockovaella haikouensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 81

Kockovaella haikouensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828736. Fig. 7A, B .

Etymology: the specific epithet haikouensis refers to the geographic origin of the type strain, Haikou county, Hainan.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal or ovoid, 1.8 –3.5 × 2.5– 5.0 μm and single, budding is polar ( Fig. 7A ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on CM, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal or some what kidney-shaped, 3.3 –5.0 × 5.0–8.3 μm ( Fig. 7B ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, soluble starch (variable), D-xylose (variable), L-arabinose (variable), D-ribose, D-glucosamine, N-Acetyl-D-glucosmine, ethanol (variable), glycerol (variable), erythritol (variable), ribitol (variable), galactitol, D-mannitol, methyl α- D-glucoside, salicin, DLlactate(variable), succinate (variable) are assimilated as sole carbon sources. L-sorbose, inulin, D-arabinose, L-rhamnose, methanol, D-glucitol, citrate, myo-inositol and hexdecane are not assimilated. Ammonium sulfate, L-lysine (variable), ethylamine hydrochloride (delayed) and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive. The major ubiquinone is Q-10.

Physiologically, Koc. haikouensis differs from the closely related species Koc. ischaemi in its inability to assimilate inulin, D-arabinose, L-rhamnose and sodium nitrite and its ability to assimilate ethylamine ( Table S1.1 ).

Typus: China, Haikou county, Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3443 T preserved in a metabolically inactive state, ex-type CBS 15478 = HKX2).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.