Tremella shuangheensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 84

Tremella shuangheensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828741. Fig. 7G .

Etymology: the specific epithet shuangheensis refers to the geographic origin of the type strain, Shuanghe county, Heilongjiang.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are subglobosal and ellipsoidal, 3.2 –4.6 × 4.0– 5.5 μm and single, budding is polar ( Fig. 7G ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, mucoid, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed and weak), Lsorbose (weak), sucrose, maltose, cellobiose, trehalose, lactose (delayed), melibiose (delayed), melezitose (delayed and weak), inulin (delayed), soluble starch (delayed and weak), D-xylose, Larabinose (weak), D-arabinose (delayed and weak), D-ribose (delayed and weak), L-rhamnose (delayed and weak), Dglucosamine (delayed and weak), N-Acetyl-D-glucosamine (delayed and weak), ethanol (delayed and weak), glycerol, erythritol, ribitol, galactitol, D-mannitol (delayed and weak), Dglucitol (delayed and weak), Methyl-α- D-glucoside (delayed and weak), salicin (delayed and weak), D-gluconate (delayed and weak), DL-lactate (delayed and weak), succinate (delayed and weak) and myo-inositol (delayed and weak) are assimilated as sole carbon sources. Raffinose, methanol, citrate and hexadecane are not assimilated. Ammonium sulfate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated. Potassium nitrate and sodium nitrite are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is delayed. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, T. shuangheensis differs from the closely related species T. globispora in its ability to assimilate lactose, melibiose, inulin and the inability to assimilate citrate ( Table S1.3 ).

Typus: China, Shuanghe county, Heilongjiang province, obtained from a leaf of an unidentified plant, Aug. 2015, Q.-M. Wang (holotype CGMCC 2.5615 T preserved in a metabolically inactive state, ex-type CBS 15561 = SH58A1).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.