Vishniacozyma melezitolytica Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 84

Vishniacozyma melezitolytica Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828742. Fig. 7H .

Etymology: the specific epithet melezitolytica refers to the physiological character of assimilating melezitose.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal, 2.6– 5.0 × 3.9–6.1 μm and single, budding is polar ( Fig. 7H ), a sediment is formed. After 1 mo at 17 °C, a pellicle and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is brownish-cream, butyrous, glistening and smooth. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose (variable), sucrose, maltose, cellobiose, trehalose, lactose, raffinose, melezitose, inulin (variable), D-xylose, L-arabinose, D-arabinose (variable), D-ribose (variable), L-rhamnose, N-Acetyl-D-glucosamine (variable), D-glucosamine (variable), ethanol, glycerol, ribitol (variable), galactitol (variable), D-mannitol, D-glucitol (variable), Methyl-α- D-glucoside (variable), salicin (weak), succinate (variable) and myo-inositol (variable) are assimilated as sole carbon sources. Melibiose, soluble starch, methanol, erythritol, D-gluconate, DL-lactate, citrate and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (variable), L-lysine, ethylamine hydrochloride (variable) and cadaverine dihydrochloride (variable) are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is variable. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, V. melezitolytica differs from the closely related species V. dimennae and V. globispora in its inability to assimilate DL-lactate and citrate and its ability to assimilate melezitose ( Table S1.4 ).

Typus: China, Hebei province, obtained from a leaf of an unidentified plant, Apr. 2007, Q.-M. Wang (holotype CGMCC 2.3472 T preserved in a metabolically inactive state, ex-type CBS 15490 = H5A3).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.