Teunia helanensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 86-87

Teunia helanensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828755. Fig. 7N .

Etymology: the specific epithet helanensis refers to the geographic origin of the type strain, Helanshan mountain, Ningxia.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid, subglobosal and ellipsoidal, 3.0– 4.7 × 4.1–6.6 μm and single, budding is polar ( Fig. 7N ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, mucoid, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed), maltose, cellobiose, trehalose, lactose, soluble starch (delayed), D-xylose, Larabinose (delayed), D-arabinose (delayed), D-ribose (delayed and weak), L-rhamnose, D-glucosamine (delayed), ethanol (delayed), glycerol (delayed), galactitol, D-mannitol, D-glucitol, salicin and succinate are assimilated as sole carbon sources. Lsorbose, sucrose, melibiose, raffinose, melezitose, inulin, methanol, erythritol, ribitol, Methyl-α- D-glucoside, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and L-lysine are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Te. helanensis differs from the closely related species Te. korlaensis in its inability to assimilate sucrose and its ability to assimilate soluble starch, D-arabinose, L-rhamnose, ethanol, erythritol, D-glucitol, succinate and L-lysine ( Table S1.7 ).

Typus: China, Helanshan mountain , Ningxia province, obtained from soil, Aug. 2009, P.J. Han (holotype CGMCC 2.4450 T preserved in a metabolically inactive state, ex-type CBS 12498 = HLS 02-1-5).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.