Pseudohyphozyma lulangensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 130-131

Pseudohyphozyma lulangensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828859. Fig. 19I, J .

Etymology: the specific epithet lulangensis refers to geographic origin of the type strain, Lulang county, Tibet.

the

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, 3.0– 4.0 × 8.4– 11.1 μm and single, budding is polar ( Fig. 19I ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is white cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 1.9– 2.7 × 5.1– 8.3 μm ( Fig. 19J ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, maltose (weak), cellobiose, trehalose, D-xylose (delayed), L-arabinose, D-arabinose (delayed and weak), D-ribose (delayed), D-glucosamine, ethanol, ribitol (delayed), D-mannitol, D-glucitol (delayed) and salicin are assimilated as sole carbon sources. Galactose, L-sorbose, sucrose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, L-rhamnose, methanol, glycerol, erythritol, galactitol, Methyl-α- D-glucoside, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, Llysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. Maximum growth temperature is 23 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ps. lulangensis differs from its closely related species Ps. bogoriensis in its inability to assimilate galactose, Lsorbose, soluble starch, glycerol and succinate and its ability to grow in vitamin-free medium ( Table S1.35 ).

Typus: China, Lulang county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2004, F.-Y. Bai (holotype CGMCC 2.2612 T preserved in a metabolically inactive state, ex-type CBS 15446 = XZ50B2).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.