Colacogloea rhododendri Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 132

Colacogloea rhododendri Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828864. Fig. 19P .

Etymology: the specific epithet rhododendri refers to Rhododendron , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, 1.0–3.8 × 4.3–15.0 μm and single, budding is polar ( Fig. 19P ), a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is prey-cream, butyrous, wrinkled and dull. The margin is entire or eroded. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (variable), sucrose, maltose, cellobiose (variable), trehalose, melezitose, inulin (variable), D- glucosamine (weak), N-Acetyl-D-glucosamine (weak), ethanol, glycerol (delayed), ribitol (variable), D-mannitol, D-glucitol (variable) and D-gluconate are assimilated as sole carbon sources. Lsorbose, lactose, melibiose, raffinose, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, methanol, erythritol, galactitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), sodium nitrite (variable), L-lysine, ethylamine hydrochloride and cadaverine dihydrochlorideare (variable) are assimilated as sole nitrogen sources. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Co. rhododendri differs from its closely related species Co. hydrangeae in its inability to assimilate salicin and its ability to assimilate glycerol ( Table S1.38 ).

Typus: China, Bomi county, Tibet, obtained from a leaf of Rhododendron lulangense, Sep. 2004 , F.-Y. Bai (holotype CGMCC 2.2770 T preserved in a metabolically inactive state, ex-type CBS 15652 = XZ10F1).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.