Slooffia globosa Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 131

Slooffia globosa Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828861. Fig. 19M .

Etymology: the specific epithet globosa refers to the globosal vegetative cells of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are globosal, 4.1– 5.9 × 4.8–5.9 μm and single, budding is polar ( Fig. 19M ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish cream, butyrous, slightly wrinkled and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose, trehalose, lactose (weak), melezitose (delayed and weak), ethanol, glycerol, D-mannitol, Methyl-α- D-glucoside (delayed and weak) and D-gluconate (weak) are assimilated as sole carbon sources. Galactose, L-sorbose, melibiose, raffinose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, Dglucosamine, N-Acetyl-D-glucosamine, methanol, erythritol, ribitol, galactitol, D-glucitol, salicin, succinate, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine (weak), ethylamine hydrochloride and cadaverine dihydrochlorideare (delayed and weak) are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 26–27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Sl. globosa differs from its closely related species Sl. tsugae in its inability to assimilate L-sorbose, Dxylose, D-glucitol, salicin, DL-lactate, succinate, citrate and sodium nitrite ( Table S1.37 ).

Typus: China, Daxinganling , obtained from soil, Aug. 2015, Q.- M. Wang (holotype CGMCC 2.5822 T preserved in a metabolically inactive state, ex-type CBS 15573 = 4 – 6).

Figures

Fig. 19. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ch. fusiformis CGMCC 2.2765T; (C, D) Ch. sorbariae CGMCC 2.2768T; (E, F) Ch. cylindrica CGMCC 2.3455T; (G) Ch. flava CGMCC 2.5611T; (H) Pseu. hydrangea CGMCC 2.2796T; (I, J) Pseu. lulangensis CGMCC 2.2612T; (K, L) Yu. longicylindrica CGMCC 2.5603T; (M) Sl. globosa CGMCC 2.5822T; (n) Co. aletridis CGMCC 2.2766T; (O) Co. hydrangeae CGMCC 2.2798T; (P) Co. rhododendri CGMCC 2.2770T. Bars = 10 μm.