Rhodosporidiobolus fuzhouensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 124

Rhodosporidiobolus fuzhouensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828841. Fig. 18C, D .

Etymology: the specific epithet fuzhouensis refers to the geographic origin of the type strain, Fuzhou county, Fujian.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and cylindrical, 3.2– 5.0 × 6.1–11.0 μm and single, budding is polar ( Fig. 18C ), a sediment is formed. After 1 mo at 17 °C, a pellicle and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pink orange, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.8– 4.9 × 6.0–9.2 μm ( Fig. 18D ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed), L-sorbose, trehalose, D-xylose (variable), D-ribose (variable), ethanol, ribitol (variable), D-mannitol (variable), D-glucitol, salicin (variable) and succinate (variable) are assimilated as sole carbon sources. Sucrose, maltose, cellobiose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, L-arabinose, D-arabinose, Lrhamnose, D-glucosamine, methanol, glycerol, erythritol, galactitol, Methyl-α- D-glucoside, gluconate, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and L-lysine (delayed and weak) are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Rh. fuzhouensis differs from its closely related species Rh. lusitaniae in its inability to assimilate galactitol, citrate, potassium nitrate and sodium nitrite ( Table S1.30 ).

Typus: China, Jinghong , Yunnan province, obtained from a leaf of an unidentified plant, Aug. 2011, Q.-M. Wang (holotype CGMCC 2.4435 T preserved in a metabolically inactive state, ex-type CBS 12492 = FJYZ2-6).

Figures

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.