Heitmania tridentata Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 124-125

Heitmania tridentata Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828844. Figs 17D and 18E .

Etymology: the specific epithet tridentata refers to the vegetative cell morphology of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical and ellipsoidal, 2.6– 3.4 × 5.9–12.0 μm and single, budding is polar, usually tridentate ( Fig. 18E ), a sediment is formed. After 1 mo at 17 °C, a part ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, maltose, trehalose, ethanol and Dmannitol (weak) are assimilated as sole carbon sources. Galactose, L-sorbose, sucrose, cellobiose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N- Acetyl-D-glucosamine, methanol, glycerol, erythritol, ribitol, galactitol, D-glucitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine and cadaverine dihydrochloride (weak) are assimilated as sole nitrogen sources. Sodium nitrite and ethylamine hydrochloride are not assimilated. Maximum growth temperature is 22–23 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, He. tridentata differs from its closely related species He. cylindrica in its inability to assimilate melezitose, glycerol, D-glucitol, succinate and ethylamine ( Table S1.31 ).

Typus: China, Milin county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5602 T preserved in a metabolically inactive state, ex-type CBS 15549 = GPS20.16B3).

Figures

Fig. 17. SEM image of vegetative cells grown in YM broth for 5 d at 17 °C. (A, B) Ros. cystopteridis CGMCC 2.2615T, A Bars = 10 μm, B Bars = 2 μm; (C) Ros. motuoensis CGMCC 2.5819T, Bars = 10 μm; (D) He. tridentata CGMCC 2.5602T, Bars = 10 μm; (E, F) He. cylindrica CGMCC 2.5650T, E Bars = 20 μm, F Bars = 5 μm; (G) Ya. terricola CGMCC 2.5820T, Bars = 10 μm; (H) Ch. rhododendri CGMCC 2.5821T, Bars = 5 μm.

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.