Rhodosporidiobolus platycladi Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 123

Rhodosporidiobolus platycladi Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828839. Fig. 16O, P .

Etymology: the specific epithet platycladi refers to Platycladus , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and ovoid, 4.0–6.2 × 5.5– 9.7 μm and single, budding is polar ( Fig. 16O ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pale cream, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal or reniform, 3.1– 5.0 × 7.0– 10.0 μm ( Fig. 16P ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, L-sorbose (weak), sucrose, maltose, cellobiose, trehalose, raffinose, melezitose, D-xylose, L-arabinose, glycerol, ribitol, D-mannitol, D-glucitol, Methylα- D-glucoside (weak) and salicin (weak) are assimilated as sole carbon sources. Galactose, lactose, melibiose, inulin, soluble starch, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, erythritol, galactitol, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite (weak), L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride (weak) are assimilated as sole nitrogen sources. Maximum growth temperature is 32 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Rh. platycladi differs from its closely related species Rh. nylandii in its inability to assimilate soluble starch, Darabinose, D-ribose, ethanol and succinate and its ability to assimilate D-xylose, L-arabinose and L-lysine ( Table S1.30 ).

Typus: China, Beijing, obtained from a leaf of Platycladus orientalis, Mar. 2006 , S.-A. Wang (holotype CGMCC 2.3118 T preserved in a metabolically inactive state, ex-type CBS 15469 = BJ6-3).

Figures

Fig. 16. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ros. petaloides CGMCC 2.3446T; (C, D) Ros. cystopteridis CGMCC 2.2615T; (E, F) Ros. motuoensis CGMCC 2.5819T; (G, H) Sp. cellobiolyticus CGMCC 2.5675T; (I, J) Sp. reniformis CGMCC 2.5627T; (K, L) Sp. ellipsoideus CGMCC 2.5619T; (M, N) Sp. primogenomicus IAM13481T; (O, P) Rh. platycladi CGMCC 2.3118T. Bars = 10 μm.