Oberwinklerozyma dicranopteridis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 126

Oberwinklerozyma dicranopteridis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828849. Fig. 18J .

Etymology: the specific epithet dicranopteridis refers to Dicranopteris , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and cylindrical, 2.2–3.7 × 6.4– 10.5 μm and single, budding is polar ( Fig. 18J ), a sediment is formed. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose, sucrose, maltose, cellobiose, trehalose, lactose, raffinose, melezitose, Dxylose, L-arabinose (delayed and weak), D-arabinose, Dglucosamine, ethanol, glycerol, ribitol, galactitol, D-mannitol, Dglucitol, Methyl-α- D-glucoside, salicin, DL-lactate (delayed and weak), succinate, citrate (delayed and weak) and myo-inositol are assimilated as sole carbon sources. Melibiose, inulin, soluble starch, D-ribose, L-rhamnose, methanol, erythritol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochlorideare assimilated as sole nitrogen sources. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, O. dicranopteridis differs from its closely related species O. straminea in its ability to assimilate cellobiose, lactose, D-arabinose, galactitol, Methyl-α- D-glucoside and salicin ( Table S1.33 ).

Typus: China, Simao county, Yunnan province, obtained from a leaf of Dicranopteris dichotoma , Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3441 T preserved in a metabolically inactive state, ex-type CBS 15476 = SM10.2).

Figures

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.