Chrysozyma pseudogriseoflava Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 126-127

Chrysozyma pseudogriseoflava Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828850. Fig. 18K, L .

Etymology: the specific epithet pseudogriseoflava refers to the similar colony morphology to that of Chrysozyma griseoflava .

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, ellipsoidal to fusiform, 3.3– 4.7 × 6.9– 9.7 μm and single, budding is polar ( Fig. 18K ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, smooth, dull and partly wrinkled. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or cylindrical, 2.3– 3.1 × 4.6– 7.7 μm ( Fig. 18L ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose, trehalose, raffinose, melezitose, ethanol and DL-lactate are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, melibiose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, glycerol, erythritol, ribitol, galactitol, Dmannitol, D-glucitol, Methyl-α- D-glucoside, salicin, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride (weak) are assimilated as sole nitrogen sources. Maximum growth temperature is 22– 23 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ch. pseudogriseoflava differs from its closely related species Ch. griseoflava in its inability to assimilate galactose, soluble starch, D-xylose, D-arabinose, glycerol, ribitol, D-glucitol, salicin and citrate and its ability to assimilate raffinose, DL-lactate and L-lysine ( Table S1.34 ).

Typus: China, Milin county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5629 T preserved in a metabolically inactive state, ex-type CBS 15564 = GPS21.6B3).

Figures

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.