Microbotryozyma swertiae Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 125

Microbotryozyma swertiae Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828846. Fig. 18G .

Etymology: the specific epithet swertiae refers to Swertia , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical and lunate, 1.7– 2.5 × 3.9–5.6 μm and single, budding is polar ( Fig. 18G ), a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, L-sorbose (delayed), sucrose, maltose, cellobiose, trehalose, lactose, melezitose, D-xylose, D-ribose (delayed and weak), glycerol, ribitol (delayed and weak), D-mannitol, D-glucitol, Methyl-α- D-glucoside, salicin and succinate (delayed and weak) are assimilated as sole carbon sources. Galactose, melibiose, raffinose, inulin, soluble starch, L-arabinose, D-arabinose, L-rhamnose, D-glucosamine, methanol, ethanol, erythritol, galactitol, D-gluconate, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate and ethylamine hydrochloride are assimilatedas sole nitrogen sources. Sodium nitrite, Llysine and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26–27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Mic. swertiae differs from its closely related species Mic. collariae in its inability to assimilate D-gluconate, DL-lactate and sodium nitrite ( Table S1.32 ).

Typus: China, Chuxiong county, Yunnan province, obtained from a leaf of Swertia yunnanensis , Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3533 T preserved in a metabolically inactive state, ex-type CBS 15495 = ZXS7.7).

Figures

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.