Chrysozyma iridis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 127

Chrysozyma iridis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828852. Fig. 18O .

Etymology: the specific epithet iridis refers to Iris , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, 2.8–3.2 × 7.2–10.3 μm and single, budding is polar ( Fig. 18O ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (weak), sucrose, maltose, cellobiose, trehalose, melezitose, inulin (weak), D-glucitol (delayed and weak), D-mannitol and salicin (delayed) are assimilated as sole carbon sources. L-sorbose, lactose, melibiose, raffinose, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, Methyl-α- D-glucoside, DLlactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate and ethylamine hydrochloride are assimilated as sole nitrogen sources. Sodium nitrite, L-lysine and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ch. iridis differs from its closely related species Ch. rhododendri in its inability to assimilate raffinose, Dxylose, L-arabinose, ethanol and Methyl-α- D-glucoside ( Table S1.34 ).

Typus: China, Bomi county, Tibet, obtained from a leaf of Iris forrestii, Sep. 2004 , F.-Y. Bai (holotype CGMCC 2.2769 T preserved in a metabolically inactive state, ex-type CBS 15461 = XZ8B3).

Figures

Fig. 18. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Rh. jianfalingensis CGMCC 2.3532T; (C, D) Rh. fuzhouensis CGMCC 2.4435T; (E) He. tridentata CGMCC 2.5602T; (F) He. cylindrica CGMCC 2.5650T; (G) Mic. swertiae CGMCC 2.3533T; (H) Ya. terricola CGMCC 2.5820T; (I) O. nepetae CGMCC 2.5824T; (J) O. dicranopteridis CGMCC 2.3441T; (K, L) Ch. pseudogriseoflava CGMCC 2.5629T; (M, N) Ch. sambuci CGMCC 2.2618T; (O) Ch. iridis CGMCC 2.2769T; (P) Ch. rhododendri CGMCC 2.5821T. Bars = 10 μm.