Meniscomyces layueensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 114

Meniscomyces layueensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828816. Figs 11E and 15D .

Etymology: the specific epithet layueensis refers to geographic origin of the type strain, Layue county, Tibet.

the

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are lunate, allantoids and falcate, 1.4– 2.6 × 7.1–10.0 μm and single, budding is polar ( Fig. 15D ), a sediment is present. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose (variable), trehalose, melezitose and succinate are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, melibiose, raffi- nose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside, salicin, D-gluconate, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine and ethylamine hydrochloride (weak) are assimilated as sole nitrogen sources. Sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 23 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Typus: China, Layue county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5818 T preserved in a metabolically inactive state, ex-type CBS 15747 = XZ100).

Figures

Fig. 11. SEM image of vegetative cells grown in YM broth for 5 d at 17 °C. (A) Fi. mali CGMCC 2.4012T, Bars = 4 μm; (B) Boe. sterigmata CGMCC 2.4539T, Bars = 5 μm; (C) St. layueensis CGMCC 2.5817T, Bars = 5 μm; (D) Pse. motuoensis CGMCC 2.5816T, Bars = 2 μm; (E) Me. layueensis CGMCC 2.5818T, Bars = 5 μm; (F) Beg. foliicola CGMCC 2.3164T, Bars = 1 μm; (G, H) Ros. petaloides CGMCC 2.3446T, G Bars = 10 μm, H Bars = 3 μm.

Fig. 15. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Phy. aceris CGMCC 2.2662T; (C) Phy. jiayinensis CGMCC 2.5669T; (D) Me. layueensis CGMCC 2.5818T; (E) Sa. melibiophila CBS 5143T; (F) Mi. ellipsoideus CGMCC 2.5664T; (G, H) Mi. rubellus CGMCC 2.4444T; (I, J) Mi. pseudomagnisporus CGMCC 2.4538T; (K, L) Sy. rhododendri CGMCC 2.2613T; (M) Cy. raffinophilum CGMCC 2.3822T; (N) Cy. terricola CGMCC 2.3823T, (O) Do. ningxiaensis CGMCC 2.4451T; (P) Beg. foliicola CGMCC 2.3164T. Bars = 10 μm.