Russula pseudopunicea C.L.Hou, H.Zhou, & G.Q.Cheng, 2023

Zhou, Hao, Cheng, Gui-Qiang, Huang, Xiao-Bo & Hou, Cheng-Lin, 2023, Two new species of Russula subgenus Heterophyllidia (Russulaceae, Russulales) from Yanshan Mountains, North China, European Journal of Taxonomy 861, pp. 185-202 : 189-192

Russula pseudopunicea C.L.Hou, H.Zhou, & G.Q.Cheng sp. nov.

MycoBank: MB844536

Figs 3–4

Diagnosis

Russula pseudopunicea sp. nov. differs from other species by the reddish brown, light brown to brownish orange pileus disc with a greyish yellow margin, small basidiospores (5.9–6.4–6.9 × 5.2–5.5–5.8 μm), short basidia (35–40–45 × 9.1–11–12 μm) and distinct position in the nrITS phylogenetic tree.

Etymology

The epithet ʻ pseudopunicea ʼ refers to its phylogenetic position relative to R. subpunicea .

Material examined

Holotype CHINA • Beijing, Miyun District, Sileng Mountains ; 40°28′23″ N, 117°6′17″ E; elev. 713 m; in broadleaf forest dominated by Betula costata Trautv .; 30 Aug. 2021; C.L. Hou, G.Q. Cheng & H. Zhao; BJTC[ BJTC ZH1392 ].

Additional material

CHINA • Beijing, Miyun District, Sileng Mountains ; 40°28′23″ N, 117°6′18″ E; elev. 710 m; in broadleaf forest dominated by Betula costata ; 30Aug. 2021; C.L. Hou, G.Q. Cheng & H. Zhao; BJTC[ BJTC ZH1389 ] • Hebei Province, Chengde City, Xinglong County, Badaziling ; 40°18′36.6″ N, 117°35′6.0″ E; elev. 879 m; in broadleaf forest dominated by Populus davidiana Dode ; 22 Aug. 2020; C.L. Hou & G.Q. Cheng; BJTC[ BJTC C335 ] • Beijing, Huairou District, Sunzhazi Village ; 40°56′33.9″ N, 116°30′25.4″ E; elev. 791 m; in coniferous and broad-leaved forest dominated by Populus davidiana and Pinus tabuliformis Carr. ; 22 Aug. 2020; C.L. Hou & G.Q. Cheng; BJTC[ BJTC C565 ].

Description

Basidiomata medium to large-sized. Pileus 35–113 mm in diam., hemispheric when young, applanate with a depressed centre at maturity; margin smooth or with indistinct striae, 3–12 mm, surface viscid when wet, cuticle peeling approximately to one-third of the pileus radius; reddish brown (#9a603e), light brown (#8e4d1e) to brownish orange (#c5994b), greyish brown (#a79e9a), margin greyish yellow (#ceb98f), pale yellow (#feeed4) to light grey-brown (#fddaa2). Lamellae cream (#ffffff) to pale yellow (#ffe7a3), adnate to adnexed, approximately 11–14 pieces/ cm near the pileus margin, not forked, lamellulae absent. Stipe 41–94 × 21–38 mm, white (#ffffff), grey-white (#d3cbba) to cream-white (#d3cfba), cylindrical, solid. Context 5–15 mm at the pileus centre, white (#ffffff), unchanging when bruised, odour not obvious, taste mild. Spore print not observed.

Basidiospores (5.2–)5.9–6.4–6.9(–7.5) × (4.8–)5.2–5.5–5.8(–6.2) μm, Q = 1.16 ± 0.16, globose to ellipsoid, ornamented with small and amyloid warts of height 0.2–0.6 μm, which are usually connected by the lower line and sometimes form a partial reticulum; hilar appendix usually conspicuous, imamyloid. Basidia (32–)35–40–44(–50) × (7.4–)9.1–10–11(–12) μm, clavate, 2-, 4-spored, thin-walled, with guttate or granular contents; basidiola clavate or subcylindrical, ca 5–10 μm wide. Pleurocystidia dispersed to moderately numerous, (54–)57–68–76(–84) × (7.1–)7.4–8.4–9.4(–10.2) μm, cylindrical, clavate or subfusiform, apically mainly obtuse, presence of heteromorphous-crystalline, occasionally banded contents, turning grey to purplish red (#694f89) in sulfovanillin. Cheilocystidia (50–)52–60–67(–76) × (5.8–)6.3–7.1–7.9(–9.2) μm, clavate, occasionally cylindrical or subfusiform, with crystalline or granulose, few banded contents. Marginal cells (15–)17–21–25(–27) × (4.0–)4.2–5.8–7.4(–11) μm, subcylindrical, often flexuous. Pileipellis orthochromatic in cresyl blue, sharply delimited from the underlying context, 210–410 μm thick, two-layered, suprapellis 60–190 μm thick, not gelatinized, composed of erect, repent or ascending and near the surface loose hyphal terminations, inflated at base and attenuated towards terminal cells; subpellis 150–270 μm thick, less gelatinized, composed of interwoven hyphae of width 2.5–5.5 μm. Hyphal terminations near the pileus margin not branched, occasionally flexuous, thin-walled; terminal cells (8.1–)9.2–22–36(–55) × (2.0–)2.4–3–3.6(–4.1) μm, subcylindrical to cylindrical, sometimes apically attenuated, subterminal cells often short or inflated, 2–8 μm wide, forked. Hyphal terminations near the pileus centre similar to those near the pileus margin, terminal cells (18–)20–29–38(–47) × (2.0–)2.4– 2.8–3.2(–3.6) μm, mostly subcylindrical. Pileocystidia near the pileus margin (34–)38–53–67(–74) × (3.0–)3.4–4.2–5(–5.4) μm, thin-walled, cylindrical, subcylindrical or narrowly fusiform, sometimes with a 3–5 μm long appendage, contents heteromorphous granulose or banded, becoming purple (#800080) in sulfovanillin; pileocystidia near the pileus centre (22–)25–39–53(–71) × (3.0–)3.2–4–4.8(–5.1) μm, thin-walled, cylindrical, subcylindrical or narrowly fusiform, apically mainly obtuse, sometimes with a 2–4 μm long appendage, contents heteromorphous-granulose. Cystidioid hyphae absent.

Figures

Fig. 3. Basidiomata and basidiospores of Russula pseudopunicea C.L.Hou, G.Q.Cheng & H.Zhou sp. nov. A–D. Basidiomata (A–B = BJTC ZH1392, holotype; C–D = BJTC ZH1389). E–H. SEM micrograph of basidiospores (BJTC ZH1392, holotype). Scale bars: A–D = 10 mm, E–H = 1 μm.

Fig. 4. Microscopic structures of lamellae of Russula pseudopunicea C.L.Hou, G.Q.Cheng & H.Zhou sp. nov., holotype (BJTC ZH1392). A. Basidia. B. Basidiola. C. Marginal cells. D. Pleurocystidia. E. Cheilocystidia. F. Pileocystidia near pileus margin. G. Hyphal terminations near pileus margin. H. Pileocystidia near pileus centre. I. Hyphal terminations near pileus centre. Scale bars = 10 μm.