Anteholosticha rectangula, Jung & Park & Kim, 2016

Jung, Jae Ho, Park, Kyung Min & Kim, Sanghee, 2016, Morphology and Molecular Phylogeny of the Soil Ciliate Anteholosticha rectangula sp. nov. from King George Island, Maritime Antarctica, Acta Protozoologica 55 (2), pp. 89-99 : 90-92

Description of Anteholosticha rectangula ( Figs 1–3 ; Table 1)

Diagnosis: Size in vivo 70–140 × 20–30 μm. Body rectangular, colorless, flexible, and slightly contractile. Four to eight macronuclear nodules; two or three micronuclei. Contractile vacuole in left mid-body. Colorless cortical granules arranged along dorsal bristles and cirri; spherical to slightly ellipsoidal, 0.5–1.0 μm in diameter in vivo. On average 21 adoral membranelles, 24 right marginal, 25 left marginal, 18 midventral which terminates at about mid-body, and 7 transverse cirri. Invariably three frontal, one buccal, two frontoterminal, and one pretransverse cirri. Three bipolar dorsal kineties with two dikinetids in front of the right marginal cirral row. Caudal cirri lacking.

Type locality: Surface soil layer partially covered by moss, King George Island, maritime Antarctica (S62°14’28.56” W58°44’52.88”). Daily records of temperature showed a range of 2.4–6.5°C in January 2013 .

Type material: One holotype slide ( NIBRPR0000106624 ) and two paratype slides ( NIBRPR0000106625 , NIBRPR0000106626 ) including protargol-stained specimens have been deposited in the National Institute of Biological Resources ( NIBR) , South Korea. Two additional paratype slides ( ACNS000273 , ACNS000274 ) of the protargol-stained specimens have been deposited in the Korea Polar Research Institute ( KOPRI) . Circles marked on the bottom of the slides indicate the relevant specimens. The SSU rDNA sequence of type population has been deposited in the GenBank under the accession number KU175624 .

Figures

Figs 1A–I. Drawings of Anteholosticha rectangula in vivo (A, D–G, I) and after protargol impregnation (B, C, H). A – ventral view of a representative specimen; B and C – ventral and dorsal views of holotype, arrows show two dikinetids; D–G – cortical granules on dorsal (D, G) and ventral sides (E, F); H – nuclear apparatus, showing variation in number and morphology; I – contractile vacuole. CG – cortical granules, CV – contractile vacuole, DB – dorsal bristles, DK1–3 – dorsal kineties 1–3, FTC – frontoterminal cirri, Ma – macronuclear nodules, Mi – micronuclei, TC – transverse cirri. Scale bars: 50 μm.

Figs 2A–J. Photomicrographs of Anteholosticha rectangula in vivo. A–C – representative individuals showing contractile vacuole (arrows) and ciliatures; D – nuclear apparatus, E–G – cortical granules in ventral (E) and dorsal (F, G) views; H–J – ventral views showing oral apparatus; arrows in I and J show buccal lip and buccal seal, respectively. CG – cortical granules, DB – dorsal bristles, Ma – macronuclear nodules, Mi – micronuclei, RMC – right marginal cirri, TC – transverse cirri. Scale bars: 100 μm (A, C, D), 5 μm (G), 10 μm (H, I).

Figs 3A–H. Photomicrographs of Anteholosticha rectangula after protargol impregnation. A and B – holotype specimen, ventral (A) and dorsal (B) view, arrow denotes pretransverse cirrus; C – dorsal view showing dorsal kineties, arrows denote two dikinetids anterior of right marginal cirral row; D and E – ventral views of anterior body showing buccal, frontal, frontoterminal, and midventral cirri; F–H – ventral views showing variation of the nuclear apparatus. DK1–3 – dorsal kineties 1–3, FC – frontal cirri, FTC – frontoterminal cirri, Ma – macronuclear nodules, Mi – micronuclei. Scale bars: 50 μm.