Orchestina luispi Izquierdo, 2017

Izquierdo, Matías Andrés & Ramírez, Martín J., 2017, Taxonomic Revision Of The Jumping Goblin Spiders Of The Genus Orchestina Simon, 1882, In The Americas (Araneae: Oonopidae), Bulletin of the American Museum of Natural History 2017 (410), pp. 1-1 : 1-

Orchestina luispi Izquierdo , new species

Figures 187–191 , 192A–D , 193 , 194D–F , 195C,

H, 196B, D, F , 197E , 198A, B, E , map 32

TYPES: Male holotype from Argentina: Buenos Aires: Ciudad Autonoma de Buenos Aires, Reserva Ecológica Costanera Sur, Camino de los sauces, mirador al Canal Sur, - 34.61694°, -58.34722°, Oct. 24, 2011, L. Zapata, G. Rubio, M. Izquierdo, M. Guala y C. Grismado, in MACN 30750, PBI_OON 42753; same data, 43, 13♀ paratypes deposited in MACN 28179, PBI_ OON 42262; same data, Margen de la Laguna Los Patos (LNP-Loc-011), 4 m, -34.608217, -58.353518, Sep. 29, 2014, beating, M. Izquierdo and L. Piacentini, 33, 5♀ paratypes deposited in AMNH, PBI_OON 51156.

ETYMOLOGY: The specific name is a noun in apposition in honour of Luis “Luispi” Piacen- tini, colleague and friend with whom we have shared many field trips and hours in the laboratory.

DIAGNOSIS: Males can easily be distinguished by the modified endites, with sclerotized curved projections (fig. 187D), and by the simple copulatory bulb (figs. 191A–C, 194D–F, 196B, D). Females resemble those of O. andianavarroi by the two long apodemes of the anterior receptaculum but can be distinguished by the less-sclerotized additional chambers of the anterior receptaculum (figs. 195C, H, 197E). In O. andianavarroi the female carapace is slightly more elevated than in this species.

MALE (PBI_OON 14809): Total length 1.13. Habitus as in figure 187A, B. CEPHALOTHO- RAX: Carapace as in figure 188A–C. Clypeus as in figure 187C. Sternum as long as wide (figs. 187D). Labium rectangular. Endites anterome- dian tip with one strong, curved projection (fig. 187D). ABDOMEN: Round, in fresh specimens, abdomen with lateral bands of purple pigment. Setae as in figure 189F, G. Spinnerets: ALS with one major ampullate gland spigot and three piriform gland spigots seemingly without bases; PMS with one minor ampullate gland spigot; PLS with two aciniform gland spigots (fig. 189A–D); epiandrum without fusules (fig. 189E). LEGS: Tarsal organ, claws, trichobothria as in figure 190. GENITALIA: Cymbium yellow, bulb yellow, spherical; embolus long; sperm duct slightly coiled (figs. 191A–F, 192A–D, 194D–F, 196B, D, F).

FEMALE (PBI_OON 14998): Total length 1.41. Habitus as in figure 187E, F. CEPHALOTHO- RAX (fig. 187A, C): Carapace texture as in figure 188F. Pedicel as in figure 188G. Sternum longer than wide (fig. 188H), yellowish white. Labium elongated hexagon (fig. 188I). Eyes as in figure 188B, D, E. ABDOMEN: Ovoid, dorsum soft portions pale orange. LEGS: Yellow. GENITA- LIA: External, internal pockets absent; anterior receptaculum with two long anterior apodemes (fig. 193A, B), three membranous chambers at base, lumen well developed; posterior receptaculum absent, posterior apodeme formed by entire plate (figs. 193, 195C, H, 197E).

DISTRIBUTION: Known from Ciudad Autonónoma de Buenos Aires and the provinces of Buenos Aires and Corrientes, Argentina (fig. 198A, B, E, map 32).

NATURAL HISTORY: Practically unknown. In the type locality the specimens were found in abundance in dry bases of Cortadeira and other grasses and trees. In field trips a couple was collected in copula. It seems to be a species found in gallery forests that are present in the last course of Uruguay River and Rio de La Plata estuary, although some specimens were found in other wetlands in Corrientes province.

OTHER MATERIAL EXAMINED: ARGENTINA: Provincia de Corrientes: Departamento de Mburucuya: P.N. Mburucuya, Camino del Uno, 64 m, -28.00083°, -58.09500°, May 27, 2011, to May 30, 2011, Rubio G., Izquierdo M. and Piacentini L., 53, 3 juveniles, sex unknown ( MACN 28273, PBI_OON 43392). Provincia de Buenos Aires: Partido de Campana: Reserva Natural Otamendi, 0.3 km instalaciones R.N. Otamendi, in remainder forest of Bacharis salicifolia and Celtis , 17 m, -34.22805°, -58.90027°, Nov. 15, 2012, A.O. Porta, G.D. Rubio, L.N. Piacentini, E.M. Soto, 1♀ ( MACN 29394, PBI_OON 42336); Reserva Natural Otamendi, 32 m, -34.22527°, -58.90000°, Apr. 22, 2006, M. Ramírez, F. Labarque, C. Sosa, 23, 1♀ ( MACN 11084, PBI_OON 14809); same data, June 10, 1997, Ramírez. Compagnucci, Grismado, Uehara, 23 ( MACN 15341, PBI_OON 14810); San Isidro: Acassuso, Reserva Municipal Refugio Natural Educativo Ribera Norte, Sauzal, 7 m, -34.46729, -58.49365, July 21, 2014, Grismado, Cristian José, Rodríguez Landó, R. et al. 13 ( MACN 32049, PBI_OON 51194); Partido de La Plata: Paseo del Bosque (-34.933333°, -57.95°), under bark of Eucalyptus sp. , June 14, 2011, J. Barneche, 1♀ ( MACN 27844, PBI_OON 43367); same data, June 15, 2011, J. Barneche, 2♀ ( MACN 27845, PBI_OON 43380); Partido de San Miguel: Campo de Mayo, Km 26 F. C. Gral. Belgrano (-34.530721°, -58.641104°), in nest of Anumbius annumbi , May 07, 2005, Paola Turienzo, 13, 1♀ ( MACN 15339, PBI_OON 14811); Partido de San Pedro: Reserva Municipal Vuelta de Obligado, -33.59861°, -59.80805°, Apr. 11, 2009, O.N. Olejnik and N. López, 1♀ ( MACN 20508, PBI_OON 14939). Ciudad Autonoma de Buenos Aires: Facultad de Veterinaria (-34.593740°, -58.483361°), in nest of Myopsitta monachus in the floor, July 07, 2009, P. Turienzo, 1♀ ( MACN 23118, PBI_OON 14998); Reserva Ecológica Costanera Sur, Laguna de los Macáes, -34.60583°, -58.34861°, Oct. 24, 2011, L. Zapata, G. Rubio, M. Izquierdo, M. Guala y C. Grismado, 13, 1♀ ( MACN 28185, PBI_OON 42141); same data, 1♀ ( MACN 28184, PBI_OON 42265); same data, grasses, -34.60472°,- 58.34916°, Oct. 24, 2011, L. Zapata, G. Rubio, M. Izquierdo, M. Guala y C. Grismado, 23 ( MACN 28182 PBI_OON 42261); 1♀ ( MACN 28182, PBI_OON 42261); same data, grasses, -34.60472°, -58.34916°, Oct. 24, 2011, L. Zapata, G. Rubio, M. Izquierdo, M. Guala y C. Grismado, 13, 1♀ ( MACN 28183, PBI_OON 42264); same data, Bosque de Tessaria integrifolia , -34.60472°, - 58.34972°, Oct. 24, 2011, L. Zapata, G. Rubio, M. Izquierdo, M. Guala y C. Grismado, 23, 5♀ ( MACN 28186, PBI_OON 42263); same data, camino de los sauces: mirador al Canal Sur, -34.61694°, -58.34722°, Nov. 04, 2011, C. Grismado and L. Zapata, 4♀ ( MACN 28237, PBI_OON 42176); same data, 13, 1♀ ( MACN 28181 PBI_OON 42260); same data, 43, 13♀ ( MACN 28179 PBI_OON 42262); same data, 13, 3♀ ( MACN 28180, PBI_OON 42266); same data, litter of Cortadeira sp, -34.61694°, -58.34722°, Nov. 04, 2011, C. Grismado and L. Zapata, 23, 4♀ ( MACN 28236, PBI_OON 42177); same data, Canal de Viamonte, -34.60458°, -58.35008°, Dec. 08, 2008, P. Turienzo, 1♀ ( MACN 26833, PBI_OON 43319); same data, Margen de la Laguna Los Patos (LNP-Loc-011), 4 m, -34.608217, -58.353518, Sep. 29, 2014, beating, M. Izquierdo and L. Piacentini, 33, 3♀ ( MRAC, PBI_OON 51170); same data, 13, 6♀ ( USNM, PBI_OON 51184); same data, 13 ( MACN 32967, PBI_OON 51072); same data 1♀ ( MACN 32966, PBI_OON 51071); same data, 1♀ ( MACN 34539, PBI_OON 42742). URUGUAY: Rivera: Cerro Miriñaque (-30.9025°, -55.550556°), bosque de cornisa, Oct. 14, 2012, G-vac (Garden-Vacuum method), M. Simo, 13 ( FCE- 7061).

Figures

FIGURE 187. Orchestina luispi. A–D. Male. E, F. Female. A–E. Habitus dorsal. B. Same, lateral. C. Cephalothorax, anterior. D. Same, ventral; arrow points to the modified endites. F. Habitus ventral. Scale bars: A, B. 0.5 mm. C–F. 0.2 mm. (PBI_OON 14809, 43367, 42264).

FIGURE 188. Orchestina luispi, SEM of female cephalothorax structures. A. Carapace, dorsal. B. Eyes, dorsal. C. Carapace, lateral. D. Eyes, anterior. E. Lateral eyes. F. Carapace texture. G. Pedicel, dorsal. H. Sternum. I. Labium. Scale bars: A, C. 200 μm. B, D, H. 100 μm. E, F, I. 20 μm. G. 50 μm. (PBI_OON 14809).

FIGURE 189. Orchestina luispi, SEM of male abdomen. A. Spinnerets, posteroventral. B. ALS. C. PLS. D. PMS. E. Epiandrium. F. Abdomen anterior. G. Detail of abdominal cuticle and setae. Abbreviations: AC, aciniform gland spigot(s); MAP, major ampullate gland spigot(s); Pi, piriform gland spigot(s). Scale bars: A. 50 μm. B, C. 10 μm. D. 5 μm. E, G. 20 μm. F. 100 μm. (PBI_OON 14809).

FIGURE 190. Orchestina luispi, SEM of male legs. A. Right claws IV, prolateral. B. Detail of setae base near claw insertion. C. Same. D. Tarsal organ IV. E. Trichobothrial pattern of tibia IV. F. Left claws I. Retrolateral view. Scale bars: A. 20 μm. B, F. 10 μm. C. 2 μm. D. 5 μm. E. 50 μm. (PBI_OON 14809).

FIGURE 191. Orchestina luispi, SEM of male palp. A. Retrolateral. B. Copulatory bulb, prolateral. C. Same, dorsal. D. Cymbium, dorsal. E. Detail of embolus. F. Detail of bulb cuticle. Scale bars: A, B. 100 μm. C. 50 μm. D. 20 μm. E. 2 μm. F. 5 μm. (PBI_OON 14809).

FIGURE 192. SEM of sperm duct. A–D. Orchestina luispi. E, F. Ariadna boesenbergi (Segestriidae). A. General view showing the different parts of the sperm duct and copulatory bulb. B. Detail of fundus and gland pores. C. Detail of the sperm duct with many pores. D. Detail of a pore. E. Similar pores in a double row in A . boesenbergi. F. Detail of pores. Scale bars: A, B. 100 μm. C. 50 μm. D. 20 μm. E. 2 μm. F. 5 μm. (PBI_OON 14809).

FIGURE 193. Orchestina luispi, female genitalia. A. Dorsal, cleared. B. SEM, ventral (cuticle partially removed). C. Same, detail of the epigastric region. D. Same, dorsal-posterior. E. Detail of the anterior receptaculum. F. Dorsal. Abbreviations: AA, apodemes of the anterior receptaculum; AR, anterior receptaculum; IT, intertracheal tube; PA, posterior apodeme; TT, tracheal tube. Asterisks indicate the additional membranous expansions of the anterior receptaculum. Scale bars: A, D, F. 50 μm. B, C. 100 μm. E. 20 μm. (PBI_OON 14809).

FIGURE 194. Orchestina spp., male palps. A–C. O . ucumar, paratype, right palp. D–F. O . luispi, paratype (PBI_OON 42262). A, D. Prolateral. B, E. Dorsal. C, D. Retrolateral. Images A–C flopped for consistency. Scale bars: 0.2 mm. (PBI_OON 14895).

FIGURE 195. Orchestina spp., female genitalia. D–H. Cleared. A, D. O . ucumar. B, E–G. O . andianavarroi. C, H. O . luispi. A–E. Ventral. H. Dorsal. G. Anterodorsal. F. Lateral. Abbreviations: AR, anterior receptaculum; M2, M3, muscles 2 and 3 respectively; PA, posterior apodeme. Asterisks indicate the membranous chambers of the anterior receptaculum. Scale bars: A. 0.25 mm. B, C. 0.1 mm. D–H. 0.025 mm. (PBI_OON 1656, 14896, 14868, 14998, 14809).

FIGURE 196. Orchestina spp., drawings of male palps. A, C, E. O . ucumar, paratype, right palp. B, D, F. O . luispi. A, B. Retrolateral. C, D. Dorsal. E, F. Scheme of sperm duct course. Images C and D flopped for consistency. Scale bars: 0.1 mm. (PBI_OON 14895, 14924, 14809).

FIGURE 197. Orchestina spp., drawings of female genitalia, ventral. A. O . moyuchi, paratype. B. O . grismadoi, paratype. C. O . ucumar. D. O . andianavarroi . E. O . luispi. Abbreviations: IP, internal pocket; LD, lateral depression; M1–M4, muscles 1–4 respectively; PA, posterior apodeme. Asterisks indicate additional membranous receptacles. Scale bars: 0.1 mm. (PBI_OON 42253, 43298, 14896, 14868, 14811).

FIGURE 198. Habitats of Orchestina in Argentina. A. Reserva Ecológica Costanera Sur (Ciudad Autónoma de Buenos Aires), type locality of O . luispi. B. Otamendi (Buenos Aires province), an additional locality were O . luispi can be found. C. Parque Nacional Calilegua (Jujuy province), type locality of O . ucumar. D. Reserva Natural Formosa (Formosa province), an additional locality of O . ucumar. E. Parque Nacional Mburucuyá (Corrientes province), additional locality where O . luispi can be found. Credits: A, C. Cristian Grismado. B. Ignacio Crudele.