Diplostomum sp.

Hoogendoorn, Coret, Smit, Nico J. & Kudlai, Olena, 2020, Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence, International Journal for Parasitology: Parasites and Wildlife 11, pp. 50-61 : 55-58

publication ID	https://doi.org/ 10.1016/j.ijppaw.2019.12.003
persistent identifier	https://treatment.plazi.org/id/03EA8781-FF80-5131-E12C-B0CC2E5155B6
treatment provided by	Felipe
scientific name	Diplostomum sp.
status

Treatment

3.3.2. Diplostomum sp. 14 sensu Locke et al. (2015)

Description ( Fig. 4d− f View Fig )

[Based on 10 live metacercariae]. Body elongate-oval, 300–472 × 175–294 (354 × 229), with maximum width at level of ventral sucker or just anterior to ventral sucker. Tegument covered with numerous tiny spines. Forebody elongate-oval, 277–443 × 178–294 (336 × 235), longer than hindbody. Hindbody rounded, short, 53–84 × 57–100 (68 × 78). Forebody/hindbody length ratio, 1:0.16–1:0.26 (1:0.21). Forebody/hindbody width ratio 1:0.24–1:0.47 (1:0.34). Pseudosuckers elongate-oval, 35–57 × 20–25 (42 × 22). Oral sucker subterminal, subspherical (n = 8), 40–51 × 40–54 (45 × 46) or transversely oval (n = 2), 44–48 × 51–54 (46 × 53). Prepharynx very short, 3–10 (5) or absent; pharynx muscular, elongate-oval, 25–37 × 17–26 (31 × 20); oesophagus short; caeca thick, long, reach posterior to holdfast organ. Ventral sucker transversely oval, postequatorial, 34–46 × 42–53 (39 × 46), smaller or equal to oral sucker [oral/ventral sucker width ratio 1:0.82–1:1.08 (1:0.96)]. Distance from ventral sucker to anterior end of body, 146–224 (177) and to posterior end of forebody, 96–168 (126). Holdfast organ subspherical, 52–67 × 57–78 (61 × 68). Distance from holdfast organ to ventral sucker, 7–15 (11). Excretory granules, medium- or large sized, scattered in forebody, but generally grouped into two lateral extracaecal and one median field. Excretory vesicle V-shaped; reserve excretory system of diplostomid type. Excretory pore subterminal, oriented ventrally.

[Based on 14 ethanol-fixed metacercariae. Measurements are provided in Table 5]. Body oval, with maximum width just anterior to ventral sucker. Tegument covered with numerous tiny spines. Forebody oval, longer than hindbody. Hindbody rounded, short Forebody/hindbody length ratio 1:0.08–1:0.26 (1:0.18), forebody/hindbody width ratio, 1:0.28–1:0.60 (1:0.40). Pseudosuckers elongate-oval. Oral sucker subterminal, subspherical. Prepharynx very short or absent; pharynx muscular, elongate-oval; oesophagus short; caeca long, thick, reach posterior to holdfast organ. Ventral sucker subspherical, postequatorial, smaller to larger than oral sucker [oral/ventral sucker width ratio 1:0.84–1:1.28 (1:1.05)]. Distance from ventral sucker to anterior end of body, 85–187 (133) and to posterior end of forebody, 89–144 (105). Holdfast organ subspherical, in posterior part of forebody. Distance from holdfast organ to ventral sucker, 5–6 (5) or holdfast organ contiguous with ventral sucker. Excretory granules, medium- ( Fig. 4e View Fig ) or large ( Fig. 4f View Fig ) sized, scattered in forebody, but generally grouped into two lateral extracaecal and one median field. Excretory vesicle Vshaped; reserve excretory system of diplostomid type. Excretory pore subterminal, oriented ventrally.

Second intermediate host: African mottled eel Anguilla labiata (Peters, 1852) ( Anguilliformes : Anguillidae ), Plain squeaker Synodontis zambezensis Peters, 1852 ( Siluriformes : Mochokidae ); Mozambique tilapia Oreochromis mossambicus (Peters, 1852) ( Perciformes : Cichlidae ).

Localities: River Phongolo Site 1 (26̊55′47″S, 32̊19′30″E) and River Phongolo Site 2 (26̊52′58″S, 32̊18′41″E).

Prevalence: 55% ( S. zambezensis ); 3 of 4 ( A. labiata ); 1 of 3 ( O. mossambicus , River Phongolo Site 2).

Intensity of infection: 1–12 metacercariae per fish ( S. zambezensis , River Phongolo Site 1); 2–6 metacercariae per fish ( A. labiata , River Phongolo Site 1); 1 metacercaria per fish ( O. mossambicus , River Phongolo Site 2).

Voucher material: 14 voucher specimens deposited in NMB P 526–530 View Materials [ NMB P 526 View Materials (2 specimens), NMB P 527 View Materials (2 specimens), NMB P 528 View Materials (3 specimens), all from S. zambezensis , River Phongolo , KwaZulu-Natal, South Africa; NMB P 529 View Materials (3 specimens) and NMB P 530 View Materials (4 specimens), both from A. labiata , River Phongolo, KwaZulu-Natal, South Africa] .

Representative DNA sequences: 28S – 3 sequences (MN813529 – MN813531), ITS1-5.8S-ITS2 – 8 sequences (MN813537 – MN813544), cox 1 – 6 sequences (MN808619 – MN808624).