Rhyacophila macedonica Karaouzas, Valladolid & Ibrahimi, 2022

Valladolid, Maria, Karaouzas, Ioannis, Ibrahimi, Halil, Arauzo, Mercedes, Stamenković, Valentina Slavevska, Dorda, Beatriz A., Hinić, Jelena, Bilalli, Astrit, Musliu, Milaim & Rey, Isabel, 2022, The Rhyacophila fasciata Group in Europe: Rhyacophila macedonica Karaouzas Valladolid & Ibrahimi (n. sp.) from Greece, Kosovo, Republic of North Macedonia and Serbia (Trichoptera: Rhyacophilidae), Zootaxa 5125 (2), pp. 101-130 : 107-109

Rhyacophila macedonica Karaouzas, Valladolid & Ibrahimi (n. sp.)

This species has been found in continental Greece, North Macedonia, Kosovo and Serbia.

Etymology. Named after the Greek region Macedonia, where the new species was first collected and identified.

Type material. Holotype ♂: GREECE. Serres , Elaionas (41.142N, 23.566E, 556 m a.s.l.), 20/ix/2018 (I. Karaouzas) ( KPC) (GenBank accession number MZ 645045 ).

Paratypes: 5 ♂♂ P + 2 ♀♀ P + 2 IP + 1L, same locality as holotype, 20/ix/2018 (I. Karaouzas) ( KPC) . 2 ♂♂ P + 1 ♀ P + 1L, Serres , Agios Ioannis, 01/v/2013 (I. Karaouzas) [1 ♂ P MNCN _ Ent 296080 ( MNCN); 1 ♂ P + 1 ♀ P ( KPC); 1L MNCN _Ent 296081 ( MNCN)] ; 1 ♂ P + 1 IP + 1 L, 21/ix/2018 (I. Karaouzas ) ( KPC) . 2 ♂♂ P + 4 ♀♀ P + 2 IP + 3L, Drama , 21/ix/2018 (I. Karaouzas) [1 ♀ P MNCN _ Ent 296082 ( MNCN); 2 ♂♂ P + 4 ♀♀ P + 2 IP + 3 L ( KPC)] .

REPUBLIC OF NORTH MACEDONIA. 1 ♂, Banjaska Reka river , above Banjane village, 11/viii/2018 (J. Hinić & V. Slavevska Stamenković) [ NMK _ SCG005 , ( MNCI)].

KOSOVO. 1 ♂, Mjak , 17/v/2017 (A. Bilalli & M. Musliu) ( KTC) . 1 ♂ + 2 ♀♀, Samakovë , 21/v/2017 (H. Ibrahimi) [1♀ MNCN _ Ent 296085 ( MNCN); 1 ♂ + 1 ♀ ( KTC)] . 3 ♂♂, Lugu i Kopilaqës , 3/xi/2019, (H. Ibrahimi) [1 ♂ MNCN _ Ent 296083 ( MNCN); 2 ♂♂ ( KTC)] . 1 ♂ + 1 ♀, Malishevë, Gjilan, 16/vii/2020, (H. Ibrahimi) ( GJTC) . 4 ♂♂, Kamenicë, 19/ix/2020 (A. Bilalli & H. Ibrahimi) ( GOTC) . 2 ♂♂, Kamenicë municipality, 19/ix/2020 (H. Ibrahimi) ( GOTC) . 2 ♂, Shushtë , 17/vii/2017 (A. Bilalli, H. Ibrahimi & M. Musliu) [1 ♂ MNCN _ Ent 296084 ( MNCN); 1 ♂ ( KTC)] . 1 ♂, Dubovë , 20/v/2020 (H. Ibrahimi) ( BNTC) .

SERBIA. 1♂ + 1 ♀, Jastrebac, 25/ix/2020 (A. Bilalli & M. Musliu) ( STC) .

Description of the larva ( Figs 2–6 , 12 .)

Biometrics of last instar larva and prepupa: Length: 12.02–18.41 mm ( = 14.61, n = 7). Maximum width of larva at metathorax: 2.53–3.38 mm ( = 3.04, n = 7). Specimens preserved in ethanol reddish brown, dorsum dark, on thorax two pale dorsolateral areas; on abdomen, two stripes of anterolateral circular pale spots, central stripe of irregular pale spots. Sclerotized areas of head and thorax pale with dark areas and spots.

Head ( Figs 2 , 12a ). Length: 1.73–1.95 mm ( = 1.82, n = 7). Width: 1.11–1.28 mm ( = 1.21, n = 7). Maximum width at posterior third of head, lateral margins slightly converging anteriorly, rounded posteriorly. Cephalic capsule ( Figs 2a–2e ), dorsally and dorsolaterally light brown, except anterior third, areas adjacent to frontoclypeal and anterior coronal sutures and anterior dorsolateral areas paler ( Fig. 2a ), in lighter specimens ( Fig. 2c ) pale in general, only posterior frontoclypeus and small spotted areas brown; ventral side pale; anteroventral edge and submentum reddish brown, posterior edge black or brown with black areas; laterally ( Fig. 2b ) with dark band in posterodorsal half with darker spots, triangular brown area with dark spots in middle, in lighter specimens ( Fig. 2d ) with only triangular brown area. Frontoclypeal apotome ( Fig. 2e ) pale; pair of oval pale brown submesal muscle attachment spots in anterior third, between setae #4; dark pigmentation in posterior third somewhat heart-shaped, with truncate anterior edges, central area paler brown, pointed anteriorly, with 4–6 oval darkly bordered brown muscle attachment spots, dark area including pair of pits #4 but not setae #6. Two-thirds of posterior dorsal area of head light brown, or pale in lighter specimens, marked with darker muscle attachment spots; at midlength one pair of light brown patches, wing-shaped, outside and paralleling posterior edge of frontal suture, in anterior extension, 2 or 3 spots parallel to suture, in middle of each patch two rows of spots perpendicular to suture; light circular area on juncture of frontal and coronal sutures, 3 or 4 pairs of spots aligned on both sides of coronal suture, two groups of spots on each side of posterior area. Three pair of pale spots around bases of setae #15, #16, and # 19–21 in darker specimens, one pale stripe in lighter specimens. Dorsal head pits with dark borders. Posterior occipital foramen black at midheight, dark brown surrounding remainder of foramen.

Mandibles ( Figs 3D, 3V ) asymmetrical, as in other rhyacophilids; left mandible slightly larger than right one. Inner blade at base of left mandible slightly convex; right mandible inner blade at base straight with small tooth in middle. Maxillolabium and labrum as in other Rhyacophila species. Labrum with small transparent area in middle of anterior edge.

Thorax ( Figs 4 , 12b ): Anterior half of pronotum pale or very light coloured. Sinuous posterior margin bordered completely by dark band, reddish brown anterior of dark band at midheight of each half. Posterior half of pronotum ( Fig. 4a ) with three dark areas separated by light zones; (1) central dark region tapered posteriorly and reaching posterior edge of sclerite, anteriorly truncate near midlength of pronotum, wider than posteriorly, lateral edges concave, pair of submesal lines of dark brown spots inside this central dark region reaching posterior edge, several dark brown spots along posterior edge; (2) pair of lateral dark areas almost triangular, each with one long edge parallel to and as long as central dark region, one parallel to and slightly longer than posterior reddish brown region, and third edge diagonal between anterior subdorsal edge and ventral subposterior edge; (3) pair of circular spots anterolateral of anterior corners of triangular lateral dark areas. Each lateral dark area with two to three transverse rows of brown circular-oval spots with dark borders parallel to posterior border; four–six brown spots around setae #5, oval spot near diagonal edge of triangular dark area and curved row of pale dots from setae #5 extending toward anterolateral corner of pronotum, curving around circular spot (spot #3) on anterior half in darker specimens ( Figs 4a, 4b ); each side with narrow black band in anterolateral angle, sometimes extending posterad as fine brown band laterally to posterolateral margins; anterior margin light brown, with several brown spots near anterior angle ( Fig. 12b ).

Legs: Similar to those of other Rhyacophila species. Colour yellowish, proximal regions of coxae, femora and distal regions of trochanters of fore- and midlegs black or dark brown, tibiae and tarsi light reddish; each inner and outer face of each femur with circular spots. Forelegs each ventrally with row of spicules, extending along from distal half of trochanter, and proximal half of femur, distal half of tibia and length of tarsus. Setae black or dark brown, some transparent in ventral area.

Abdomen ( Figs 5, 6 , 12c, 12d ): Similar to that of other species of Rhyacophila . In preserved larvae reddish brown dorsally, two stripes of anterolateral circular pale spots, central stripe of irregular pale spots, star shaped, ventral side pale. Widths of anterior abdominal segments similar, narrower in posterior segments. Lateral abdominal gills in tufts of 30–35 fine filaments. Dorsal sclerite of segment IX ( Figs 5 , 12c ) light coloured, with continuous dark band on anterior edge and pair of discontinuous black bands on posterior edge; band of anterior edge black in lateral areas, brown in middle, with conspicuous pits in both sides; in darker specimens with semicircular coloured area in anterior middle; some dark spots near black bands on posterior edge, semicircular hyaline zone in middle posterior edge. Anal prolegs typical of group, each with long sword process ( Figs 6a, 6b , 12d ). Anal claws each with three or four teeth on ventral edge ( Figs 6a, 6c ), basoventral hook black basally and reddish brown apically ( Figs 6b , 12d ).

Figures

FIGURES 2–6. Larva of Rhyacophila macedonica Karaouzas, Valladolid & Ibrahimi (n. sp.). 2a–2e, head: 2a, dark coloration, dorsal; 2b, dark coloration, right lateral; 2c, pale coloration, dorsal; 2d, pale coloration, right lateral; 2e, frontoclypeus, dorsal. 3D–3V, mandibles from last instar larval exuviae, left (l) and right (r): 3D, dorsal; 3V, ventral. 4a–4b, prothorax: 4a, dorsal; 4b, left hemisclerite, left lateral. 5, abdominal tergite IX. 6a–6c, details of anal prolegs: 6a, right anal claw, right lateral; 6b, detail of basolateral plate with sword process and basoventral hook, right lateral; 6c, detail of right claw, right lateral. Scale bars: unlabelled = 1 mm; ● = 0.5 mm.

FIGURES 12, 13. Larvae of Rhyacophila macedonica Karaouzas, Valladolid & Ibrahimi (n. sp.) and Rhyacophila fasciata Hagen 1859. 12, R. macedonica: 12a, head, dorsal; 12b, prothorax, dorsal; 12c, abdominal tergite IX, dorsal; 12d, left anal proleg, left lateral. 13, R. fasciata: 13a, head, dorsal. 13b, prothorax, dorsal; 13c, abdominal tergite IX, dorsal; 13d, left anal proleg, left lateral. Scale bars: 1 mm.