Echiniscus meridionalis, Gąsiorek, 2023

Gąsiorek, Piotr, 2023, Eventually tested: Phylogenetic position of “ Testechiniscus ” meridionalis (Murray, 1906) (Heterotardigrada) revealed, Zoologischer Anzeiger 304, pp. 49-60 : 52-54

3.2. Redescription of Echiniscus meridionalis stat. rev.

Figs. 2 – 7 , Tables 2–4 , Supplementary Material 4 with raw morphometric data.

ZooBank registration number: urn:lsid:zoobank.org:pub:8A36A0BF- AA4F–427F–980B-E70DE960695E.

3.2.1. Terra typica

South Orkney Islands. Material examined. South Shetland Islands (see Table 1 for detailed data).

3.2.2. Etymology

From Latin meridionalis = southern, referring to the distribution of the species in Antarctic and sub-Antarctic archipelagos. An adjective in nominative singular.

3.2.3. Adult females (i.e. from the third instar onwards; measurements and statistics in Table 2 )

Body small and plump ( Figs. 2A and 3A ), light yellow to dark orange in alive specimens. Eyes dark red, minute and dissolving immediately in Hoyer’ s medium. Peribuccal cirri relatively long with respect to the body length ( Figs. 2A , 3A and 4A ); cirrus A of a moderate length, i.e. reaching approximately 1/3 of the body length; all cephalic cirri with well-delineated cirrophores. Cephalic papillae (secondary clavae) slightly longer than (primary) clavae, both barrel-shaped ( Fig. 4A ). Body appendage configuration A-C-C d -D-D d -E, with most appendages formed as long, flexible cirri. Short, but robust spines present in positions D and D d ( Figs. 2A and 3A ).

Dorsal plate sculpturing comprises: (I) poorly elevated epicuticular granules, with a strong tendency to merging in all plate portions but the anterior portions of paired segmental plates I–II, median plate 2, and the much reduced median plate 3, where they are separate ( Figs. 2A and 3A ), ( II) pseudopores in the form of delicate depressions distributed irregularly throughout the dorsum, and ( III) pores, present mainly in the cephalic, scapular ( Figs. 2A and 3A ), and occasionally in the caudal (terminal) plates. Pores may be absent as well. Cephalic plate narrow, with the usual chalice-like anterior incision. Cervical plate even narrower, but clearly present and rectangular, tightly joined with the scapular plate. Scapular plate with a distinct faceting-like pattern formed by a system of poorly elevated ridges ( Figs. 2A , 3A and 4A ); lateralmost plate portions weakly demarcated from the central portion by a similar ridge ( Fig. 4A ) that replaced a usual suture/incision. Median plate 1 unipartite, m2 bipartite and much larger, m3 developed as a triangular sculptured surface dominated by the caudal plate. Paired segmental plates I–II identical in size and shape. Caudal plate with large triangular posterior incisions ( Fig. 2A ) that are joined by a central semicircular ridge ( Figs. 2A , 3A and 4A ). A weakly marked ridge perpendicular to the former may be developed, delineating a cross-like epicuticular structure.

Ventral plate armature variously developed, as plates in some individuals may be almost indiscernible, but the general plate formula stands as VIII:2-1-1-2-2-2-2-2 ( Figs. 4B and 5A ). Particularly the plate in the second row can be hardly identifiable in PCM ( Fig. 5A ). Ventral plates are smooth ( Fig. 4B ), but the endocuticular pillars are easily observable in PCM ( Fig. 5A ). Besides, no granulation present on venter. Hexapartite gonopore and tripartite, large anus ( Figs. 4B and 5B ). Legs with evident pulvini embedded in proximal portions of limbs and pedal plates in median portions, developed as wrinkled areas with sparse and occasional pores ( Figs. 4A and 6B ). Long spine I present ( Figs. 4A and 6A ); papilla IV large and barrel-shaped, dentate fringe on leg IV with numerous irregular teeth, typically interconnected at their bases ( Figs. 2A , 4A and 6B ). Claws with branches typically curved, those of legs IV slightly higher than the remaining ones. Claw spurs heteromorphic, as spurs IV are more divergent from the branches compared with spurs I– III. The size and position of spurs on claw branches (ca. 1/3–2/5 of the claw height) identical on all claws ( Fig. 6 ).

3.2.4. Adult males (i.e. from the second/third instar onwards, see remarks; measurements and statistics in Table 3 )

Sexual dimorphism evinces in several characters of males: thinner and shorter body ( Figs. 2B and C , 3B , 4C–D ), prominent and swollen clavae ( Figs. 2B and C , 3B , 4C–D and 5B ), and often better visible (darker in PCM) ventral plates ( Fig. 5B ). Rarely, all appendages but A and E may be formed as spines ( Fig. 2B ). Gonopore circular.

3.2.5. Juveniles (i.e. the second instar; measurements and statistics in Table 4)

Qualitatively similar to sexually mature females, and smaller than both sexes. Gonopore absent.

3.2.6. Larvae (i.e. the first instar; measurements and statistics in Table 4)

Qualitatively similar to later life stages, but smaller than juveniles. Body appendage formula: A-Cd- E, all formed as cirri ( Fig. 7 ). Two claws per leg. Gonopore and anus absent.

3.2.7. DNA markers

Single haplotype was found in 18S rRNA ( OQ 472387), 28S rRNA ( OQ 472389), ITS-1 ( OQ 476878), ITS-2 ( OQ 476879), and two in COI ( OQ 472391–2; p -distance = 0.2%).

3.2.8. Differential diagnosis

A strong sexual dimorphism, the unusual dorsal plate sculpturing (in some specimens, epicuticular granules may be barely elevated above the surface of plates, and in an initial observation in PCM such exemplars seem to be sculptureless), and the ventral armature covering an entire venter, replacing the endocuticular granulation typical for Echiniscus , together with the geographic range embracing solely Antarctic and sub-Antarctic islands ( Fig. 8 ), make E. meridionalis stat. rev. easily recognisable and well-differentiated morphologically from all other Echiniscus spp.

3.2.9. Remarks

The species can be frequently encountered on King George Island, being one of the two commonest echiniscids in that location (the other is Barbaria jenningsi , often co-inhabiting the same mosses and lichens, see Table 1 ). An undescribed Pseudechiniscus species, eutardigrades Acutuncus antarcticus , Diphascon rudnickii , and unidentified species of the Mesobiotus furciger group ( Short et al., 2022) are other relatively common taxa that co-occur with E. meridionalis stat. rev. However, irrespective of its ubiquity in the area, the population density must be low given that typically few representatives were found in samples ( Table 1 ). Ventral armature is already developed in E. meridionalis stat. rev. at sexually immature life stages (i.e. in larvae and juveniles), which often does not take place in the Echiniscidae .

Figures

Fig. 2. Dorsal plate sculpturing of Echiniscus meridionalis stat. rev. (PCM, adults in dorsal view): A – female, B, C – males. Scale bars = 20 μm.

Fig. 5. Ventral plate sculpturing of Echiniscus meridionalis stat. rev. (PCM, adults): A – female, B – male. Roman numerals indicate subsequent plate rows. Scale bars = 20 μm.

Fig. 6. Claws of Echiniscus meridionalis stat. rev. (adults): A – claws I with spine I (PCM), B – claws IV, dentate collar and papilla IV (PCM), C – claws I (SEM), D – claws IV (SEM). Scale bars in μm.

Fig. 7. Larva of Echiniscus meridionalis stat. rev. (PCM, dorsolateral view). Scale bar = 20 μm.

Fig. 3. Habitus of Echiniscus meridionalis stat. rev. (SEM, adults in dorsal view): A – female, B – male. Scale bars = 50 μm.

Fig. 4. Habitus of Echiniscus meridionalis stat. rev. (SEM, adults and a juvenile in non-dorsal view): A – female in lateral view, B – juvenile in ventral view (Roman numerals indicate subsequent plate rows), C, D – males in lateral view. Scale bars = 50 μm.

Fig. 8. Distribution of two emblematic Antarctic echiniscids: purple stars – Echiniscus meridionalis stat. rev. (South Orkney Islands, South Shetland Islands, Argentine Islands), yellow star – “Testechiniscus” macronyx (South Georgia). Map downloaded from https://www.freeworldmaps.net.