Seinura persica, Adeldoost, Yaser, Heydari, Ramin, Miraiez, Esmail, Jalalinasab, Parisa & Asghari, Ramezan, 2016

Adeldoost, Yaser, Heydari, Ramin, Miraiez, Esmail, Jalalinasab, Parisa & Asghari, Ramezan, 2016, Description of two new species of Seinura Fuchs, 1931 (Nematoda: Aphelenchoididae) from Iran, Zootaxa 4168 (3), pp. 541-556 : 545-549

Seinura persica n. sp.

( Figs 3 & 4 )

Measurements. See Table 1 .

Description. Female. Body slender and slightly curved ventrally upon fixation. Cuticle ca 1 µm thick with fine transverse annuli most visible in anterior region. Lateral fields with four incisures. Anterior end set off from body contour by a definite constriction, 3–4 µm high and 6.0–8.5 µm wide. Stylet (18–20) µm long without basal swelling, conus about 41.5% of total stylet length with opening oriented ventrally. Procorpus cylindrical, 1.4–1.8 times longer than stylet length, ending in a muscular, rectangular metacorpus (median bulb), 1.4–1.6 times longer than wide, with anterior granular part occupying 23–30% of its total length and the posterior part moderately muscular with valve posterior to center. Pharyngo-intestinal junction not visible. Glandular region of pharynx well developed, overlapping intestine dorsally, ca 3.2–5.8 times metacorpus length or ca 5.7–6.5 times body diameter at metacorpus level. Excretory pore with little variation in position, mostly between metacorpus base and its valve. Hemizonid not observed. Nerve ring located posterior to metacorpus, surrounding both intestine and pharynx. Reproductive system outstretched with one column of oocytes. Spermatheca mostly oval in shape and frequently filled with sperm. Post-uterine sac 2.6–3.5 times body diameter at vulval region. Vulva not protuberant, vagina slightly oblique. Intestine granulate and ending in a generally functional rectum and a distinct anus. Tail elongateconical 3.3–5.8 times body diameter at the anus, curved ventrally, attenuated asymmetrically, usually has a shallow depression dorsally, ending in a sharply pointed tip.

Male. Body slender, J-shaped after heat relaxation, posterior end more ventrally curved. General body form (i.e. head, stylet, pharynx and excretory pore) as for female. Testis single, expanded anteriorly, not reflexed. Spicules arcuate, separate, ca 1.8 times longer than capitulum width, lamina/calomus complex smoothly and symmetrically curved, rostrum prominent, triangular-shaped with narrowly rounded end, condylus well-developed Seinura persica n. sp. Seinura hyrcania n. sp.

Characters Female Male Gorgan population Guilanpopulation

Holotype Paratypes Paratypes Holotype Paratypes -

– 10 2 12 12

Anal (cloacal) body diam 10.7 10.7 ± 0.7 (10–12) 10, 11 10 11.5 ± 1 (10–14) 9 ± 0.7 (8–10) Tail length 36 46 ± 7 (36–55) 48, 49 56 65 ± 7.2 (51–82) 53 ± 8.4 (43–66) Spicule (chord) – – 16,15 – – –

Conus length/stylet length (%). 2: Median bulb from anterior end/pharynx length (%). 3: Post-uterine sac length. 4: Post-uterine sac length/vulva to anus (%).

and slightly dorsally bent. Distal end of spicules bluntly rounded, cucullus absent. A total of seven papillae present: a single midventral precloacal papilla (P1), one pair subventral papillae (P2) at level of or just posterior to cloacal aperture and two subventral pairs of postcloacal papillae (P3 & P4) being located adjacent to the tail end. Tail conical, ending in a long ventral mucron ca 9–10 µm length with a pointed tip.

Diagnosis and relationships. The female of Seinura persica n. sp. is characterized by a distinctly offset lip region, four incisures in lateral field, length of stylet 18–20 µm and lacking basal swelling, excretory pore opening between base of the metacorpus and its valve, post-uterine sac occupying 65.2 (57–81) % of vulva-anus distance, and elongate-conical tail with pointed tip and length 3.3–5.5 times body diameter at the anus region. Based on presence of post uterine sac, plain base of stylet and V ratio, the new species is close to S. obscura Grewal, Siddiqi & Atkey, 1992 , S. onondagensis Kaisa, 2000 , S. oswegoensis Van der Linde, 1938 and S. oostenbrinki Husain & Khan, 1967 .

Compared with S. obscura , the new species differs by position of excretory pore (opening anterior to vs posterior to base of metacorpus), shorter stylet 19.5 (18–20) vs 24 (20–26) µm, longer post-uterine sac 54.8 (50– 60) vs 22 (18–30) µm and female tail shape (elongate-conical with pointed tip vs elongate with a filiform terminus). Seinura persica n. sp. can be distinguished from S. onondagensis by having an offset cephalic region vs continuous, longer post-uterine sac 54.8 (50–60) vs (14–38) µm, shape of the tail terminus (pointed vs filiform) and lower c´ratio 4.4 (3.3–5.5) vs 8 (7.0–9.0). Compared to S. oswegoensis (data from Grewal et al., 1991) the new species differs in having a shorter tail length 46 (36–55) vs 68 (56–80) µm, shape of the tail terminus (pointed vs filiform), and arrangement of oocytes (in a single column vs 3–4 columns).The new species can be distinguished from S. oostenbrinki by position of excretory pore (anterior to vs posterior to base of metacorpus), arrangement of oocytes (in a single column vs two columns), and longer post-uterine sac (occupying 57–81% of vulva-anus distance vs 33–50%).

Type host and locality. Seinura persica n. sp. was collected from bark samples of a cypress tree ( Cupressus sempervirens L.) in Bovan canyon, Nurabad city, Fars Province (GPS coordinates: N 30° 04', E 51° 64'), Iran.

Type material. Holotype female (Slide ASP001), three paratype females and two paratype males (Slides ASP001, ASP002 and ACI003) deposited in the nematode collection of the Department of Plant Protection , College of Agriculture and Natural Resources, University of Tehran, Iran . Three paratype females deposited at the nematode collection of the Royal Belgian Institute of Natural Sciences, Brussels. Two paratype females deposited at the National Nematode Collection of the Department of Nematology , Iranian Research Institute of Plant protection, Tehran, Iran .

Etymology. The species epithet refers to Latin name of Iran, the origin of the species, from where the type specimens were collected.

Figures

FIGURE 3. Line drawings of Seinura persica n. sp. A: Female entire body; B: Female anterior end; C: Female anterior region; D: Male entire body; E: Female posterior body; F: Male posterior body arrows show caudal papillae (P 1 = precloacal papilla, P 2 = subventral papillae at level of cloacal aperture, P 3 & P 4 = postcloacal papillae); G: Position of excretory pore.

FIGURE 4. Photomicrographs of Seinura persica n. sp. A: Female anterior region; B: Female anterior end; C: Male anterior end; D: Mid body arrow shows excretory pore; E: Four lateral lines arrows show lateral line region; F: Female posterior region arrows show end of post-uterine sac and position of anus; G: Female posterior end; H: Male posterior region; I: Male papillae (arrows), P 1, P 2, P 3 and P 4; J: Spicule in detail. (Scale bars: A & H = 20 µm; B, C, D, E, F, G, I & J = 10 µm).

Tables

TABLE 1. Morphometrics of Seinura persica n. sp. and Seinura hyrcania n. sp. from Iran. All measurements are in µm and in the form: average ± SD (range).

L	499	519 ± 35 (467–592) 511, 517	501	534 ± 41 (437–600)	433 ± 58 (355–521)
a	27.7	28 ± 1.6 (26–32) 28.4, 28.7	29	26 ± 2 (23–30)	29 ± 2 (25–35)
b	6.9	6.7 ± 0.4 (6.2–7.2) 6.4, 6.6	7	6.9 ± 0.4 (6.2–7.3)	7.4 ± 1 (6.2–9)
b'	3	2.9 ± 0.2 (2.7–3.2) 2.7, 2.8	2.9	2.9 ± 0.3 (2.5–3.3)	3.3 ± 0.4 (3–4.1)
c	13.9	11.3 ± 1.3 (9.8–14) 10.4, 10.8	9	8.5 ± 1.5 (5.5–10)	8.4 ± 1 (7–10)
c'	3.3	4.4 ± 0.8 (3.3–5.5) 4.5, 4.8	5.6	5.8 ± 1.3 (3.6–8)	6 ± 1.1 (5–8)
V or T	75.6	75 ± 0.9 (73.8–76.8) –	71	71 ± 2 (66–74)	71 ± 1.5 (68–72)
Head height	4	3.8 ± 0.4 (3–4) 3.5, 4	4	4 ± 0.1 (4–4.5)	3.2 ± 0.4 (3–4)
Head width	8	8 8,8	8.5	8 ± 0.3 (7.5–8.5)	6.5 ± 0.5 (6–7)
Stylet M 1	19 42.1	19.5 ± 0.9 (18–20) 19, 20 41 ± 2.6 (37.5–45) 40, 40	20 42.5	21.5 ± 1.5 (18–24) 41.5 ± 2.2 (38–45)	18 ± 1.5 (16–21) 41 ± 3.5 (33–47)
Pharynx	72	77.5 ± 5.5 (65–83) 78, 81	73	77.5 ± 7 (60–86)	59 ± 5 (50–65)
Overlap	96	100 ± 8.8 (86–111) 102, 109	97	109 ± 17 (71–131)	72 ± 17 (51–82)
Median bulb MB 2	63.0 87.5	65 ± 4.7 (55–70) 62, 68 84 ± 3 (80–90) 79.5, 84	64 84	68 ± 6 (52–75) 88 ± 2.3 (85–93)	51 ± 4 (43–57) 87 ± 1.2 (86–89)
Nerve ring	83.8	84 ± 3.5 (77–87) 85, 86	74	79 ± 8 (60–84)	67.1 ± 4.1 (61–72)
Excretory pore	58	63 ± 3.6 (55–66) 65, 64	60	63 ± 5 (54–73)	48 ± 6 (43–58)
Max. body diam. PUS 3	18 55	18.6 ± 1.2 (17–21) 19,19 54.8 ± 3.9 (50–60) –	17 17	20 ± 1.7 (17–23) 19 ± 4 (12–25)	15 ± 1.5 (13–17) 18 ± 3 (14–24)
PUS/max. body diam. PUS/ V–A% 4	2.9 60	3 ± 0.2 (2.7–3.5) – 65.2 ± 6.5 (57–81) –	1 19	1 ± 0.2 (0.6–1.3) 21 ± 5 (13–30)	1.3 ± 0.2 (0.9–1.6) 24 ± 3 (17–30)
Vulva –anus	86	85 ± 9 (74–100) –	88	90 ± 8.5 (70–102)	79 ± 13 (61–98)
Testis or ovary length	177	168 ± 12 (147–183) 165, 166	183	205 ± 29 (156–274)	147 ± 32 (114–200)